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21.
Keli Li G. F. Veen Freddy C. ten Hooven Jeffrey A. Harvey Wim H. van der Putten 《Ecology letters》2023,26(1):37-52
Soils contain biotic and abiotic legacies of previous conditions that may influence plant community biomass and associated aboveground biodiversity. However, little is known about the relative strengths and interactions of the various belowground legacies on aboveground plant–insect interactions. We used an outdoor mesocosm experiment to investigate the belowground legacy effects of range-expanding versus native plants, extreme drought and their interactions on plants, aphids and pollinators. We show that plant biomass was influenced more strongly by the previous plant community than by the previous summer drought. Plant communities consisted of four congeneric pairs of natives and range expanders, and their responses were not unanimous. Legacy effects affected the abundance of aphids more strongly than pollinators. We conclude that legacies can be contained as soil ‘memories’ that influence aboveground plant community interactions in the next growing season. These soil-borne ‘memories’ can be altered by climate warming-induced plant range shifts and extreme drought. 相似文献
22.
Wim Van de Vrie Sylke A. M. Van der Heyden Eric E. O. Gheuens Amelie M. Bijma Ernst A. De Bruijn Richard L. Marquet Allan T. Van Oosterom Alexander M. M. Eggermont 《Cancer immunology, immunotherapy : CII》1993,37(5):337-342
The development of resistance to anticancer drugs urges the search for different treatment modalities. Several investigators have reported the concomitant development of drug resistance and resistance to natural killer (NK), lymphokine-activated killer (LAK) or monocyte/macrophage cell lysis, while others described unchanged or even increased susceptibility. We investigated this subject in the rat colon carcinoma cell line, CC531-PAR, which is intrinsically multidrug-resistant (MDR), and in three sublines derived from this parental cell line: a cell line with an increased MDR phenotype (CC531-COL), a revertant line from CC531-COL (CC531-REV), which demonstrates enhanced sensitivity to anticancer drugs of the MDR phenotype, and an independently developed cisplatin-resistant line (CC531-CIS). In a 4-h51Cr-release assay we found no difference in susceptibility to NK cell lysis. No significant differences in lysability by adherent LAK (aLAK) cells were observed in a 4-h assay. In a prolonged 20-h51Cr-release assay an enhanced sensitivity to aLAK-cell-mediated lysis was observed in the revertant, P-glycoprotein-negative cell line and in the cisplatin-resistant cell line (CC531-CIS). None of the cell lines was completely resistant to lysis by aLAK cells. Therefore, a role for immunotherapy in the treatment of drug-resistant tumors remains a realistic option. 相似文献
23.
Molecular cloning of a gene involved in glucose sensing in the yeast Saccharomyces cerevisiae 总被引:6,自引:1,他引:5
Linda Van Aelst Stefan Hohmann Botchaka Bulaya Wim de Koning Laurens Sierkstra Maria José Neves Kattie Luyten Rafael Alijo José Ramos Paola Coccetti Enzo Martegani Neuza Maria de Magalhães-Rocha Rogelio Lopes Brandão Patrick Van Dijck Mieke Vanhalewyn Peter Durnez Arnold W. H. Jans Johan M. Thevelein 《Molecular microbiology》1993,8(5):927-943
24.
The flash-induced absorbance change measured at 518 nm (P515) in intact chloroplasts consists of at least 4 kinetically different components. Here the non-electrochromic component, either called phase d or reaction 3, is studied in some detail. The effect of DCMU, DQH2 and DBMIB on the amplitude of reaction 3 and the turnover of cytochrome f and P700 have been monitored, suggesting an involvement of photosystem 1 in the activation of the non-electrochromic absorbance change. This is confirmed by the parallel oscillation pattern found in P700 rereduction and the amplitude of reaction 3. 相似文献
25.
26.
Photobiology of microorganisms: how photosensors catch a photon to initialize signalling 总被引:2,自引:1,他引:1
The afa-3 gene cluster determines the formation of an afimbrial adhesive sheath that is expressed by uropathogenic as well as diarrhoea-associated Escherichia coli strains. It contains six genes ( afaA–afaF ), among which the afaE3 gene is known to code for the structural AfaE-III adhesin (previously designated AFA-III), whereas no role has yet been identified for the afaD gene product. The afa-3 gene cluster is closely related to the daa operon that codes for an adhesin, the F1845 adhesin, which is highly related to the AfaE-III adhesin; however, unlike the AfaE-III adhesin, F1845 is a fimbrial adhesin. Reported in this work is the construction of chimeras between the afa-3 and daa operons. Analyses of the phenotypes conferred by these afa-3 / daa chimeric clusters allowed us to conclude that the biogenesis of a fimbrial or an afimbrial adhesin is fully determined by the amino acid sequence of the AfaE-III and F1845 adhesins. Moreover, the role of the AfaD product in the biosynthesis of the afimbrial sheath was assessed by immunogold and immunofluorescence experiments. The AfaD and the AfaE-III products were purified and used to raise rabbit and mouse antisera. Similar to AfaE-III, AfaD was found to be a surface-exposed protein as well as an adhesin; both AfaD and AfaE-III are concomittantly expressed by the bacterial cell. These results demonstrate, for the first time, that the afimbrial adhesive sheath expressed by pathogenic E. coli is composed of two adhesins. 相似文献
27.
28.
Enhancement of the light-triggered electrical response in plant cells following their de-energization with uncouplers 总被引:1,自引:0,他引:1
Light-triggered membrane potential changes in cells of a liverwort Anthoceros are greatly enhanced by the ionophorous uncouplers nigericin and monesin. Stimulation of the light-triggered electrical response (LTER) by nigericin occurred concomitantly with inhibition of a slow decline in the chlorophyll fluorescence, which suggests that the transmembrane pH gradient in thylakoids is not essential for generation of LTER at the plasma membrane. The extent of monensin-stimulated LTER remained high under a diminished driving force for the ionophore-induced proton-cation exchange across the plasma membrane (elevation of the external Na+ concentration from 1 to 50 m M ), which indicates that energy uncoupling in chloroplasts is more related to the electric response enhancement than the induction of the H+ /K+ (Na+ ) exchange at the plasma membrane. Enhancement of LTER by ionophores occurs in parallel with stimulation of light-triggered pH changes (alkalinization) in the vicinity of the cell surface, which suggests an association of trans-membrane H+ fluxes with LTER. The results are consistent with the hypothesis that illumination produces a temporary inhibition of the plasma membrane H+ pump with a subsequent activation of gated channels and transient rapid depolarization of the cell. 相似文献
29.
Acid atmospheric deposition can cause losses of metal nutrients from the organic layer of a soil. The size of these losses
depend on the sizes of the different pools in which the metals are present, as these pools differ in mobility. The metal pools
in an organic soil layer of a Douglas fir forest in the Netherlands subjected to acid deposition were determined by means
of extractions and percolations. Na was mainly dissolved and exchangeably adsorbed, K dissolved, exchangeably adsorbed and
present in the soil microbial biomass, Ca exchangeably adsorbed and present in organic precipitates, Mg exchangeably adsorbed
and present in the soil biomass, and Mn exchangeably adsorbed and present in inorganic precipitates. The main part of the
metals was exchangeably adsorbed. The adsorption affinity increased in the order Na < K < Mg < Mn ≈ Ca. The vertical distribution
of the metals in the organic layer showed that all metals were continuously lost from the organic layer. The differences between
the metals in retention and vertical distribution patterns were in agreement with their differences in deposition rate, pool
distribution, and exchange affinity. Since the metals were mainly exchangeably adsorbed, and the acidifying cations dominated
the atmospheric deposition, acid deposition and cation exchange must be processes that strongly affect the losses of metals
from this organic soil layer. R F Huettl Section editor 相似文献
30.
Therapy of bovine ocular squamous-cell carcinoma with local doses of interleukin-2: 67% complete regressions after 20 months of follow-up 总被引:1,自引:1,他引:0
F. W. Willem Den Otter Graham Hill Wim R. Klein Jan Willem Koten Peter A. Steerenberg Pieter H. M. De Mulder Christopher Rhode Rachel Stewart Joop A. J. Faber E. Joost Ruitenberg Victor P. M. G. Rutten 《Cancer immunology, immunotherapy : CII》1995,41(1):10-14
We have tested the therapeutic potency of peritumorally injected low doses of interleukin-2(IL-2). Seventy tumours of the bovine ocular squamous-cell carcinoma (BOSCC), 1–3 cm in diameter, were treated with 5000, 20 000 or 200 000 U IL-2 from Eurocetus (Chiron) to find the optimal dose for treatment. Injections were given peritumorally on Monday to Friday on 2 consecutive weeks. The size of the tumours was measured before treatment and 1, 3, 4, 9 and 20 months after treatment. After 9 months complete regression was observed in 89% of the tumours treated with 5000 U IL-2, 80% treated with 20 000 U and 67% treated with 200 000 U. After 20 months, there was complete regression of 35%, 31% and 67% of the tumours respectively. The 9-and 20-month results of the 200 000-U treatment are significantly better than those of the 5000-U and 20 000-U treatments taken together. This protocol may be useful to treat advanced inoperable tumours (e.g. of the nasopharynx or skin) of human patients. 相似文献