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221.
Protein phosphorylation in respiring slices of guinea-pig cerebral cortex. Evidence for a role for noradrenaline and adenosine 3'':5''-cyclic monophosphate in the increased phosphorylation observed on application of electrical pulses. 下载免费PDF全文
1. Exposure of slices of cerebral cortex from guinea pigs to electrical pulses for 10s or to noradrenaline, 5-hydroxytryptamine or histamine increases the rate of phosphorylation of unidentified proteins in the tissue; the increases in protein phosphorylation due to electrical pulses and noradrenaline were non-additive, whereas the increases due to pulses and 5-hydroxytryptamine or histamine were additive. 2. The stimulating effects of electrical pulses and noradrenaline on protein phosphorylation were antagonized by the beta-adrenergic blocking agents L-propranolol, dichloroisoprenaline, practolol and ICI 66082, but not by the alpha-adrenergic blocking agents, phentolamine and phenoxybenzamine. 3. The increase in protein phosphorylation associated with electrical pulses was antagonized by 10 mum-trifluoperazine and 0.5 mum-prostaglandin E1. 4. It is postulated that under the experimental conditions used the action of electrical pulses on protein phosphorylation is mediated by noradrenaline acting through a beta-adrenergic receptor mechanism probably involving adenylate cyclase. 相似文献
222.
I F Williams R Harwood M E Grant 《Biochemical and biophysical research communications》1976,70(1):200-206
White erythrocyte membranes, or ghosts, were monoconcave discocytes when incubated in 50mM N-tris (hydroxymethyl) methyl-2-aminoethane sulfonic acid titrated to pH 7.4 with triethanolamine. If 3mM MgCl2 was included in the incubation medium, the ghosts were predominantly echinocytes. The echinocytic form could also be induced by Co++, Ni++, Li+, Na+, K+, and tetramethylammonium ion, all as chloride salts. The concentration of cation necessary for 50% of the ghosts to be echinocytes was correlated with the hydrated charge density of the cation with the most highly charged cations being the most effective. The cations Ca++, Sr++, Ba++ and La+++, (also as chloride salts) did not induce the normal echinocytic form, but at high levels induced a few misshapen forms with some resemblance to echinocytes. Instead Ca++, Sr++, Ba++ and La+++ suppressed the formation of echinocytes in the presence of Mg++ and other ions. This suggests the presence of a specific Ca++ binding site important to shape control in the erythrocyte membrane. 相似文献
223.
224.
Small discrete spikes appearing on the electrocardiogram (ECG) of a man with a pacemaker turned out to be fasciculation potentials picked up from leg muscles. To find out how common these spikes were all routine ECGs made in one month were reviewed. Spike potentials were found in six patients, and in each case they originated from somatic musculature. Spikes could nearly always be detected on ECGs that were recorded subsequently in patients known to have lower motor neurone lesions that produced fasciculation. Thus the presence of these fasciculation spikes may suggest underlying neuromuscular disease; this possibility should be recognised by those who record ECGs so that the spikes are not suppressed by overuse of the filter. 相似文献
225.
Brian A. Laishes Gary M. Williams 《In vitro cellular & developmental biology. Plant》1976,12(7):521-532
Summary The conditions for obtaining representative, primary adult rat hepatocyte cultures were explored. The methods applied included
enzymatic liver perfusion which was nondestructive to hepatocytes, the prevention of aggregation of dissociated cells and
the selective attachment of viable cells. These procedures yielded a recovery of 50% of the liver cells which gave rise to
cultures representing 14% of the total liver cells. The cultures were composed of homogeneous epithelial-like cells cytologically
similar to hepatocytes and possessed a number of liver-specific enzymes. There was virtually no cell division initially and
most cells died between 24 and 48 hr. Insulin enhanced the attachment of the liver cells, altered their morphology, but did
not prolong cell survival.
This study was supported by grant no. BC 133 from the American Cancer Society. 相似文献
226.
Reduction of the active center disulfide bond in the flavoprotein pig heart lipoamide dehydrogenase generates two sulfur moieties which are chemically inequivalent in the 2-electron reduced form of the enzyme. Thus 1 cysteine residue is at least 13-fold more reactive than its partner toward iodoacetamide at pH 7.6. This selectivity was demonstrated by reaction of the 2-electron reduced enzyme with a low concentration of iodo[1-14C]acetamide under anaerobic conditions. The formation of a monolabeled derivative is accompanied by the reappearance of a spectrum of oxidized bound flavin, clearly different from that of the native enzyme. Alkylation of the remaining cysteine residues with iodo[12C]acetamide enabled the isolation of a tryptic version of the active center disulfide peptide. A single chymotryptic cleavage between the 2 alkylated cysteine residues generated a cationic and an anionic fragment containing 7% and 93% of the radioactivity of the purified tryptic peptide, respectively. The monolabeled derivative is catalytically inactive toward reduced or oxidized lipoamide, but is approximately 2-fold better as a transhydrogenase than the native protein using NADH and acetylpyridine adenine dinucleotide as substrates. Anaerobic titration with NADH leads to reduction of the flavin with concomitant formation of long wavelength absorption of low intensity. No intermediate reduced states were detected in this titration analogous to the red 2-electron form observed with the native enzyme. Similarly, intermediates during reduction of the enzyme by 1 eq of dithionite have not been detected. 相似文献
227.
B. S. Chertow G. A. Williams Georgiann R. Baker R. D. Surbaugh G. K. Hargis 《Experimental cell research》1975,93(2):388-394
To determine the role of subcellular organelles in hormone secretion, we studied the interaction of low calcium concentration (low Ca), retinol (vitamin A, vit A), vinblastine (VB), and cytochalasin B (CB) in parathyroid hormone (PTH) secretion. Bovine parathyroid tissue pieces were incubated in media containing the above agents. Vit A stimulated PTH release to a mean of 170% of control. This effect of vit A was diminished when tissues were simultaneously stimulated with low Ca and, furthermore, absent when tissues were pre-incubated in low Ca.VB had no effect on low Ca-stimulated secretion, but did inhibit vit A-induced secretion in the presence of low Ca.CB stimulated PTH secretion to a mean of 150% of control during the second and third hours of incubation. CB had at least an additive effect with low Ca in stimulating PTH secretion, with a more prompt and greater response than seen in normal calcium. VB did not inhibit the acute effect of CB on secretion in normal calcium media, but did inhibit CB-induced secretion during the third hour of incubation.None of the agents stimulated the release of lysosomal cathepsin D, and vit A and CB did not stimulate the release of LDH.Our results suggest that; (1) vit A and low Ca stimulate PTH secretion through a common pathway involving the cell membrane; (2) CB stimulates PTH secretion through a separate effect on the cell membrane or submembrane microfilaments, which normally retards secretion of PTH; and (3) microtubular proteins may facilitate basal secretion of PTH, but are not involved in low Ca-stimulated secretion of PTH. 相似文献
228.
Improved chemical synthesis and enzymatic assay of delta-1-pyrroline-5-carboxylic acid. 总被引:8,自引:0,他引:8
Δ1-Pyrroline-5-car?ylic acid, an intermediate in both the biosynthesis and degradation ofl-proline, has been synthesized by the periodate oxidation of hydroxylysine and isolated as a pure compound, as indicated by enzymatic assay with pyrroline-5-car?ylate reductase fromEscherichia coli. Some features of the instability in solution ofΔ1-pyrroline-5-car?ylic acid have been studied, leading to the conclusion that the rate of decomposition is sensitive to concentration of the compound. Colorimetric assay witho-aminobenzaldehyde was found to be an inadequate measure of the pyrroline compound in partially decomposed solutions. 相似文献
229.
J T Maher L G Jones L H Hartley G H Williams L I Rose 《Journal of applied physiology》1975,39(1):18-22
Hormonal responses to graded exercise of eight low altitude residents were examined at sea level (SL) and after 1 (acute) and 11 (chronic) days at 4,300 m (HA). Caloric, water, and electrolyte intakes were controlled, as were temperature and humidity. Blood was sampled at rest and during light and moderate upright bicycle exercise (20 min at 40% and 75% of maximal O2 uptake, respectively). Mean VO2 max at HA was 27% lower than at SL. Resting plasma levels of aldosterone (Aldo), renin, and angiotensin II (A II) were significantly lower (P smaller than 0.05) on day 1 at HA compared to SL, but returned to SL values by day 11. Plasma cortisol values at rest were similar at SL and HA and were not significantly altered by light or moderate exercise. Renin, A II, and Aldo rose progressively with increasing workload in each environment. With acute HA, renin and Aldo were lower than at either SL or chronic HA. The chronic HA levels tended to approximate SL findings, implying adaptation. The data suggest that aldosterone is predominantly under the control of the renin-angiotensin system during graded exercise at sea level and that the response of this system is altered on acute high-altitude exposure. 相似文献
230.