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101.
Christian Biémont Cristina Vieira Christine Hoogland Géraldine Cizeron Catherine Lœvenbruck Claude Arnault Jean-Pierre Carante 《Genetica》1997,100(1-3):161-166
To investigate the main forces controlling the containment of transposable elements (TE) in natural populations, we analyzed
the copia, mdg1, and 412 elements in various populations of Drosophila melanogaster and D. simulans. A lower proportion of
insertion sites on the X chromosome in comparison with the autosomes suggests that selection against the detrimental effects
of TE insertions is the major force containing TE copies in populations of Drosophila. This selection effect hypothesis is
strengthened by the absence of the negative correlation between recombination rate and TE copy number along the chromosomes,
which was expected under the alternative ectopic exchange model (selection against the deleterious rearrangements promoted
by recombination between TE insertions). A cline in 412 copy number in relation to latitude was observed among the natural
populations of D. simulans, with very high numbers existing in some local populations (around 60 copies in a sample from Canberra,
Australia). An apparent absence of selection effects in this Canberra sample and a value of transposition rate equal to 1–2
× 10-3 whatever the population and its copy number agree with the idea of recent but temporarily drastic TE movements in local populations.
The high values of transposition rate in D. simulans clearly disfavor the hypothesis that the low amount of transposable elements
in this species could result from a low transposition rate.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
102.
Hummel Herman Bogaards Roelof Bek Tatiana Polishchuk Lennard Amiard-Triquet Claude Bachelet Guy Desprez Michel Strelkov Peter Sukhotin Alex Naumov Andrei Dahle Salve Denisenko Stanislav Gantsevich Michael Sokolov Kirill Wolf Lein de 《Hydrobiologia》1997,355(1-3):127-138
The stress sensitivity, determined in copper exposureexperiments and in survival in air tests, and thegenetic structure, measured by means of isoenzymeelectrophoresis, were assessed in populations of theBaltic clam Macoma balthica (L.) from itssouthern to its northern distribution limit, in orderto test the hypotheses that near the distributionlimit the clams would be more stress sensitive andwould have a lower genetic variability. Thepopulations in west and north Europe show a stronggenetic resemblance. The populations in the sub-ArcticWhite Sea are genetically slightly different, and showa low stress sensitivity. The populations in theArctic Pechora Sea are genetically very distant fromthe other populations, and show the lowest stresssensitivity. Near the southern distribution limit, inagreement with the hypotheses, genetic variability islow and stress sensitivity high. On the other hand, incontrast to expectation, near the northerndistribution limit, in the populations of the PechoraSea, the genetic variability was higher, thus notreduced, and the stress sensitivity was low comparedto all other populations. Yet, it remains a questionif such is due to gradual physiologicalacclimatization (and ongoing differential selection)or to genetic adaptation. 相似文献
103.
Louis Prusse Yvon C. Chagnon France T. Dionne Claude Bouchard 《Obesity (Silver Spring, Md.)》1997,5(1):49-61
An update of the human obesity gene map up to October 1996 is presented. Evidence from Mendelian disorders exhibiting obesity as a clinical feature, single-gene mutation rodent models, quantitative trait loci uncovered in crossbreeding experiments with mouse, rat, and pig models, association and case-control studies with candidate genes, and linkage studies with genes and other markers is reviewed. All chromosomal locations of the animal loci are converted into human genome locations based on syntenic relationships between the genomes. A complete listing of all these loci reveals that only 4 of the 24 human chromosomes are not yet represented, i.e., 9, 18, 21, and Y. Several chromosome arms are characterized by the presence of several putative loci. The following arms include at least three such loci: 1p, 1q, 3p, 4q, 6p, 7q, 8p, 8q, 11p, 11q, 15q, 20q, and Xq. Studies with negative association and linkage results are also reviewed. 相似文献
104.
Abstract Conditional cold-sensitive mutations in Era, an essential Escherichia coli GTPase, were isolated. Localized random polymerase chain reaction (PCR) mutagenesis employing Taq and T7 DNA polymerases under error prone amplification conditions was exploited to generate mutations in the era gene. A plasmid exchange technique was used to identify conditional cold-sensitive mutations in Era that give rise to defective cell growth below 30 °C. Three recessive missense mutations in Era, N26S, A156D, and E200K, were isolated. All three mutations are located at residues conserved in Era homologues from Streptococcus mutans and Coxiella burnetii . 相似文献
105.
106.
Jacqueline S. Weinman Claude L. Gallien Dominique P. Rainteau Michèle Guyot Serge J. Weinman Jacques G. Demaille 《Developmental biology》1984,101(1):73-85
Calmodulin isolated and purified to homogeneity from young larvae is very similar to that obtained from adult Pleurodeles waltlii and these proteins are almost identical to previously described vertebrate calmodulins. During P. waltlii development, an increase in total individual calmodulin content is observed after the heart beating stage. In dorsal axial muscle, calmodulin level which is very high at the beginning of larval life (premetamorphosis) decreases strikingly in the first part of prometamorphosis. Such an evolution is observed in Ambystoma mexicanum too. Then, a significant increase occurs during metamorphosis. In contrast, calmodulin level in P. waltlii cardiac ventricular muscle increases continuously from hatching to the end of metamorphic climax. Thyroxine treatment which promotes precocious metamorphosis in P. waltlii and experimental metamorphosis in neotenic A. mexicanum, induces a rapid and significant increase in muscle calmodulin concentration. 相似文献
107.
A polyclonal antiserum was raised against highly purified RNA polymerase II from soybean embryos. Pure RNA polymerase II was fractionated on sodium dodecyl sulfate-polyacrylamide gels and transferred onto nitrocellulose sheets, incubated with the immune antiserum and then with iodinated protein A. Autoradiograms showed that the immune antiserum recognized all subunits of RNA polymerase II. Subunits 42, 27 and 16 kdalton were particularly reactive. Application of this transfer technique to protein extracts from soybean embryos or from cultured soybean cells allowed the identification of subunits of RNA polymerase II in the extracts. Analysis of the staining of the bands on the autoradiograms for increasing amounts of pure RNA polymerase II demonstrated that the transfer was quantitative, so that standard curves could be drawn to estimate the unknown amounts of enzyme in the extracts.Abbreviations DEAE
diethylaminoethyl
- SDS
sodium dodecyl sulfate 相似文献
108.
The inhibitory effects of nikkomycin, polyoxin B, and UDP were tested on particulate chitin synthetase activity (UDP-2-acetamido-2-deoxy-D-glucose: chitin-4-B-acetamidodeoxy-D-glucosyltransferase, E.C.2.4.1.16) fromNeurospora crassa. Two approaches were used: (a) inhibitors were tested for their individual effects on chitin synthetase activity; (b) paired combinations of inhibitors were examined to establish whether the compounds affected inhibition by binding at the same enzyme site. The first method showed that the three compounds are linear competitive inhibitors, i.e. each competes directly with the substrate for binding. Ki app values were: UDP, 0.8 mM; polyoxin B, 32 M; and nikkomycin, 2 M. The second method showed that the inhibitors compete with each other for binding; thus they bind at the same site. The pyrimidine nucleoside moiety of these inhibitors is an essential component for effective inhibition, but the potency of inhibition is critically dependent on the conformation of the side group attached to carbon 5 of the ribose sugar. 相似文献
109.
Trichoderma reesei was grown using purified cell walls ofNeurospora crassa as a primary source of carbon. The resulting culture medium contained an undefined mixture ofN. crassa cell-wall digesting enzymes. Protoplasts (cell lacking wall) were formed when youngN. crassa hyphae were treated withTrichoderma mixture. The vast majority of protoplasts resynthesized cell-wall material when washed free of cell-wall digesting enzyme; of these, about 40% regenerated a mycelium. 相似文献
110.
25 populations from Turkey and one of Syria belonging to theSabulina section of the genusMinuartia have been karyologically examined. New chromosome numbers have been recorded forM. mesogitana andM. hybrida subsp.turcica, and a new variety was found in theM. hybrida complex. The origin of the taxa with n = 23 and n = 35 is discussed. 相似文献