排序方式: 共有86条查询结果,搜索用时 15 毫秒
51.
52.
Harholt J Sørensen I Fangel J Roberts A Willats WG Scheller HV Petersen BL Banks JA Ulvskov P 《PloS one》2012,7(5):e35846
Spike mosses are among the most basal vascular plants, and one species, Selaginella moellendorffii, was recently selected for full genome sequencing by the Joint Genome Institute (JGI). Glycosyltransferases (GTs) are involved in many aspects of a plant life, including cell wall biosynthesis, protein glycosylation, primary and secondary metabolism. Here, we present a comparative study of the S. moellendorffii genome across 92 GT families and an additional family (DUF266) likely to include GTs. The study encompasses the moss Physcomitrella patens, a non-vascular land plant, while rice and Arabidopsis represent commelinid and non-commelinid seed plants. Analysis of the subset of GT-families particularly relevant to cell wall polysaccharide biosynthesis was complemented by a detailed analysis of S. moellendorffii cell walls. The S. moellendorffii cell wall contains many of the same components as seed plant cell walls, but appears to differ somewhat in its detailed architecture. The S. moellendorffii genome encodes fewer GTs (287 GTs including DUF266s) than the reference genomes. In a few families, notably GT51 and GT78, S. moellendorffii GTs have no higher plant orthologs, but in most families S. moellendorffii GTs have clear orthologies with Arabidopsis and rice. A gene naming convention of GTs is proposed which takes orthologies and GT-family membership into account. The evolutionary significance of apparently modern and ancient traits in S. moellendorffii is discussed, as is its use as a reference organism for functional annotation of GTs. 相似文献
53.
Mackenzie AK Pope PB Pedersen HL Gupta R Morrison M Willats WG Eijsink VG 《Applied and environmental microbiology》2012,78(16):5935-5937
We demonstrate that two characteristic Sus-like proteins encoded within a polysaccharide utilization locus (PUL) bind strongly to cellulosic substrates and interact with plant primary cell walls. This shows associations between uncultured Bacteroidetes-affiliated lineages and cellulose in the rumen and thus presents new PUL-derived targets to pursue regarding plant biomass degradation. 相似文献
54.
Jens Øbro Bernhard Borkhardt Jesper Harholt Michael Skjøt William G. T. Willats Peter Ulvskov 《Transgenic research》2009,18(6):961-969
Despite the wide occurrence of pectin in nature only a few source materials have been used to produce commercial pectins.
One of the reasons for this is that many plant species contain pectins with high levels of neutral sugar side chains or that
are highly substituted with acetyl or other groups. These modifications often prevent gelation, which has been a major functional
requirement of commercial pectins until recently. We have previously shown that modification of pectin is possible through
heterologous expression of pectin degrading enzymes in planta. To test the effect of simultaneous modification of the two main neutral pectic side chains in pectic rhamnogalacturonan
I (RGI), we constitutively expressed two different enzymes in Arabidopsis thaliana that would either modify the galactan or the arabinan side chains, or both side chains simultaneously. Our analysis showed
that the simultaneous truncation of arabinan and galactan side chains is achievable and does not severely affect the growth
of Arabidopsis thaliana. 相似文献
55.
Wiethölter N Graessner B Mierau M Willats WG Knox JP Moerschbacher BM 《Carbohydrate research》2003,338(5):423-431
In contrast to the typical type I cell wall of the dicot plants, the type II cell wall of the commelinoid monocot plants is known to be relatively poor in pectins. Assuming a critical role for the remaining pectins in terms of cell wall architecture and/or as a reservoir of signalling molecules, we have compared different protocols for the isolation of the main pectin polymer, homogalacturonan, from wheat leaf cell walls. Pectin was detected in these cell walls immunochemically using the monoclonal antibodies JIM5 and JIM7, and biochemically by monosaccharide analysis. The Ca(++)-chelators CDTA and imidazole extracted a pectin rich fraction from isolated cell walls which was however contaminated with significant amounts of hemicelluloses. Pretreatment of the cell walls with anhydrous hydrogen fluoride at controlled low temperatures followed by HF/ether- and water-extraction prior to imidazole-extraction of pectins yielded a purer homogalacturonan fraction. The near absence of rhamnosyl residues proved that the isolated homogalacturonan fraction was free of rhamnogalacturonans. If HF-solvolysis was performed at -23 degrees C, the resulting homogalacturonan had a degree of methyl esterification identical to that of the pectins in the initial wheat cell wall. The antibodies JIM5 and JIM7 as well as PAM1 and LM5 proved that the isolated homogalacturonan had a low methyl ester content, was polymeric and free of galactan side chains. We can thus isolate native homogalacturonan from the type II wheat cell walls with the original in muro pattern of methyl esterification still intact, to further investigate e.g., its degradability by plant or microbial pectic enzymes. 相似文献
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Altered middle lamella homogalacturonan and disrupted deposition of (1-->5)-alpha-L-arabinan in the pericarp of Cnr, a ripening mutant of tomato
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Orfila C Seymour GB Willats WG Huxham IM Jarvis MC Dover CJ Thompson AJ Knox JP 《Plant physiology》2001,126(1):210-221
Cnr (colorless non-ripening) is a pleiotropic tomato (Lycopersicon esculentum) fruit ripening mutant with altered tissue properties including weaker cell-to-cell contacts in the pericarp (A.J. Thompson, M. Tor, C.S. Barry, J. Vrebalov, C. Orfila, M.C. Jarvis, J.J. Giovannoni, D. Grierson, G.B. Seymour [1999] Plant Physiol 120: 383-390). Whereas the genetic basis of the Cnr mutation is being identified by molecular analyses, here we report the identification of cell biological factors underlying the Cnr texture phenotype. In comparison with wild type, ripe-stage Cnr fruits have stronger, non-swollen cell walls (CW) throughout the pericarp and extensive intercellular space in the inner pericarp. Using electron energy loss spectroscopy imaging of calcium-binding capacity and anti-homogalacturonan (HG) antibody probes (PAM1 and JIM5) we demonstrate that maturation processes involving middle lamella HG are altered in Cnr fruit, resulting in the absence or a low level of HG-/calcium-based cell adhesion. We also demonstrate that the deposition of (1-->5)-alpha-L-arabinan is disrupted in Cnr pericarp CW and that this disruption occurs prior to fruit ripening. The relationship between the disruption of (1-->5)-alpha-L-arabinan deposition in pericarp CW and the Cnr phenotype is discussed. 相似文献
58.
In-situ analysis of pectic polysaccharides in seed mucilage and at the root surface of Arabidopsis thaliana 总被引:1,自引:0,他引:1
Pectic polysaccharides are a complex set of macromolecules of the primary cell wall matrix with distinct structural domains.
The biosynthesis, organisation and function of these domains within cell wall matrices are poorly understood. An immersion
immunofluorescence labelling technique was developed for the in-situ analysis of pectic polysaccharides at the surface of
seeds and seedlings of Arabidopsis thaliana (L.) Heynh., and used to investigate the occurrence of pectic homogalacturonan (HG) and rhamnogalacturonan-I (RG-I) epitopes.
Seed mucilage appeared to consist of two regions: a highly methyl-esterified HG was a major component throughout the mucilage,
while an inner region with relatively low porosity was stabilized by calcium-based HG cross-linking. The small size and transparency
of Arabidopsis roots allowed the occurrence of pectic HG and RG-I epitopes at root surfaces to be directly determined on whole-mount preparations.
Pectic epitopes were not distributed evenly over root surfaces and were notably absent from lateral root apices and from the
surface of root hairs. The use of defined antibody probes in the immersion immunolabelling protocol will be useful for the
analysis of the influence of growth conditions and genetic factors on pectic polysaccharides in Arabidopsis.
Received: 13 July 2000 / Accepted: 15 September 2000 相似文献
59.
Willats WG McCartney L Steele-King CG Marcus SE Mort A Huisman M van Alebeek GJ Schols HA Voragen AG Le Goff A Bonnin E Thibault JF Knox JP 《Planta》2004,218(4):673-681
A monoclonal antibody (LM8) was generated with specificity for xyloglacturonan (XGA) isolated from pea (Pisum sativum L.) testae. Characterization of the LM8 epitope indicates that it is a region of XGA that is highly substituted with xylose. Immunocytochemical analysis indicates that this epitope is restricted to loosely attached inner parenchyma cells at the inner face of the pea testa and does not occur in other cells of the testa. Elsewhere in the pea seedling, the LM8 epitope was found only in association with root cap cell development at the root apex. Furthermore, the LM8 epitope is specifically associated with root cap cells in a range of angiosperm species. In embryogenic carrot suspension cell cultures the epitope is abundant at the surface of cell walls of loosely attached cells in both induced and non-induced cultures. The LM8 epitope is the first cell wall epitope to be identified that is specifically associated with a plant cell separation process that results in complete cell detachment.Abbreviations
DAA
Days after anthesis
- 2,4-D
2,4-Dichlorophenoxyacetic acid
-
ELISA
Enzyme-linked immunosorbent assay
-
GalA
Galacturonic acid
-
HGA
Homogalacturonan
-
HPAEC
High-performance anion-exchange chromatography
-
HPSEC
High-performance size-exclusion chromatography
-
RG-I
Rhamnogalacturonan-I
-
RG-II
Rhamnogalacturonan-II
-
XGA
Xylogalacturonan 相似文献
60.
Joel S Riley Giovanni Quarato Catherine Cloix Jonathan Lopez Jim O'Prey Matthew Pearson James Chapman Hiromi Sesaki Leo M Carlin João F Passos Ann P Wheeler Andrew Oberst Kevin M Ryan Stephen WG Tait 《The EMBO journal》2018,37(17)
During apoptosis, pro‐apoptotic BAX and BAK are activated, causing mitochondrial outer membrane permeabilisation (MOMP), caspase activation and cell death. However, even in the absence of caspase activity, cells usually die following MOMP. Such caspase‐independent cell death is accompanied by inflammation that requires mitochondrial DNA (mtDNA) activation of cGAS‐STING signalling. Because the mitochondrial inner membrane is thought to remain intact during apoptosis, we sought to address how matrix mtDNA could activate the cytosolic cGAS‐STING signalling pathway. Using super‐resolution imaging, we show that mtDNA is efficiently released from mitochondria following MOMP. In a temporal manner, we find that following MOMP, BAX/BAK‐mediated mitochondrial outer membrane pores gradually widen. This allows extrusion of the mitochondrial inner membrane into the cytosol whereupon it permeablises allowing mtDNA release. Our data demonstrate that mitochondrial inner membrane permeabilisation (MIMP) can occur during cell death following BAX/BAK‐dependent MOMP. Importantly, by enabling the cytosolic release of mtDNA, inner membrane permeabilisation underpins the immunogenic effects of caspase‐independent cell death. 相似文献