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41.
Cristoph Zimmer Gerhard Luck Eckhard Birch-Hirschfeld Roland Weiss Federico Arcamone Wilhelm Guschlbauer 《Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression》1983,741(1):15-22
Different binding affinities of various distamycin analogs including the deformylated derivative with poly(dA-dC)·poly(dG-dT) were investigated using CD measurements. The inhibitory effect of distamycins on the DNAase I cleavage activity of DNA duplexes strongly supports the binding data. The base specificity of the ligand interaction with duplex DNA depends on the chain length of distamycin analogs. Netropsin, distamycin-2 and the deformylated distamycin-3 show no binding to dG·dC containing sequences at moderate ionic strength and are classified as highly dA·dT specific. In contrast distamycin having three, four or five methylpyrrolecarboxamide groups also forms more or less stable complexes with dG·dC-containing duplexes. These ligands possess a lower basepair specificity. The correlation between binding behavior and oligopeptide structure shows that presence of the number of hydrogen acceptor and donor sites determines the basepair and sequence specificity. The additional interaction with dG·dC pairs becomes essential when the number of hydrogen acceptor sites exceeds n = 3. 相似文献
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Dynamics of the cytoskeleton in Amoeba proteus 总被引:3,自引:0,他引:3
Fluorescein-labeled muscle actin was microinjected into Amoeba proteus and followed during intracellular redistribution by means of the image-intensification technique. The fully polymerization-competent protein becomes part of the endogenous actomyosin system undergoing dynamic changes over time periods of several hours. Single-frame analysis of long-term sequences enabled the direct demonstration of both the contractile activities and morphological transformations of microfilaments in normally locomoting, immobilized and phagocytozing specimens. In normally locomoting cells the filament layer undergoes continuous changes in spatial distribution depending on the actual pattern of cytoplasmic streaming and cell shape. The highest degree of differentiation is always maintained in the intermediate region between the front and the uroid, thus indicating this segment of the cortex to be the most important site in generating motive force for pseudopodium formation and ameboid movement. In immobilized cells contracted by the application of ruthenium red or relaxed by different anesthetics, the filament layer forms a continuous thick sheath beneath the cell surface or becomes completely disintegrated. In phagocytozing cells the local polymerization of actin at the tip of pseudopodia forming the food-cup and around the nascent phagosome points to a significant participation of the actomyosin system in the process of capturing and constricting prey organisms. Although our results provide clear evidence for the overall importance of motive force generation according to the hydraulic pressure theory, some motile phenomena exist in Amoeba proteus that cannot exclusively be explained by this mechanism. 相似文献
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Stimulation by 6-N,2′-O-dibutyryladenosine 3′:5′-cyclic monophosphate and glucocorticoids of phosphoenolpyruvate carboxykinase in the isolated perfused rat liver (Short Communication)
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Wieland B. Huttner Wilhelm Krone Hans J. Seitz Wolfgang Tarnowski 《The Biochemical journal》1974,142(3):691-693
Dibutyryl cyclic AMP stimulated the activity of phosphoenolpyruvate carboxykinase in perfused livers of rats, fed on a low-protein diet, linearly over a 6h period. The enzyme activity was also significantly elevated by dexamethasone, the effect being considerably lower than that of the cyclic nucleotide. Since the time-course of phosphoenolpyruvate carboxykinase activity in response to dibutyryl cyclic AMP resembled that observed after dibutyryl cyclic AMP injection into intact animals, it is suggested that induction of the enzyme in vivo is due to a direct action of the cyclic nucleotide on the liver. Combined administration of dibutyryl cyclic AMP and glucocorticoids did not lead to an additive increase of liver phosphoenolpyruvate carboxykinase activity, either in vivo or in the perfused organ. 相似文献
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The feasibility of radioisotope-fueled circulatory support systems depends on the ability of the body to dissipate the reject heat from the power source driving the blood pump as well as to tolerate chronic intracorporeal radiation. Our studies have focused on the use of the circulating blood as a heat sink. Initial in vivo heat transfer studies utilized straight tube heat exchangers (electrically and radioisotope energized) to replace a segment of the descending aorta. More recent studies have used a left ventricular assist pump as a blood-cooled heat exchanger. This approach minimizes trauma, does not increase the area of prosthetic interface with the blood, and minimizes system volume. Heat rejected from the thermal engine (vapor or gas cycle) is transported from the nuclear power source in the abdomen to the pump in the thoracic cavity via hydraulic lines. Adjacent tissue is protected from the fuel capsule temperature (900 to 1200 degrees F) by vacuum foil insulation and polyurethane foam. The in vivo thermal management problems have been studied using a simulated thermal system (STS) which approximates the heat rejection and thermal transport mechanisms of the nuclear circulatory support systems under development by NHLI. Electric heaters simulate the reject heat from the thermal engines. These studies have been essential in establishing the location, suspension, surgical procedures, and postoperative care for implanting prototype nuclear heart assist systems in calves. The pump has a thermal impedance of 0.12 degrees C/watt. Analysis of the STS data in terms of an electrical analog model implies a heat transfer coefficient of 4.7 x 10(-3) watt/cm(2) degrees C in the abdomen compared to a value of 14.9 x 10(-3) watt/cm(2) degrees C from the heat exchanger plenum into the diaphragm. 相似文献
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Zusammenfassung Wird einzelligen Algen (Ankistrodesmus braunii) nach Vorbelichtung in anschließender Dunkelheit 32P-markiertes Phosphat geboten, so tritt gegenüber Dauerdunkel eine erhebliche Förderung der 32P-Einlagerung auf. Die nach Vorbelichtung bestimmte Markierung der aufgetrennten Phosphatfraktionen ähnelt sehr derjenigen im Dauerlicht. Die erhöhte Dunkelphosphorylierung nach Vorbelichtung hängt von der CO2-Konzentration, von der Lichtintensität und der Zeit der Vorbelichtung ab. Unter den vorliegenden Bedingungen waren 7 min Vorbelichtung zur maximalen Förderung nötig. Die Halbwertzeit des Abklingens betrug etwa 4 min.Aus den Experimenten geht hervor, daß durch die Belichtung der Algen auch in vivo ein Zustand gebildet wird, der noch nach Belichtung eine Zeitlang im Dumkeln eine Erhöhung der 32P-Einlagerung erlaubt.Es wird diskutiert, ob es sich einerseits um die Bildung einer im Licht reduzierten Substanz R handeln könnte, die für eine begrenzte Zeit in Dunkelheit noch einen cyclischen, mit Phosphorylierung gekoppelten Elektronentransport aufrechterhalten kann. Andererseits könnte durch die Vorbelichtung ein energiereiches Zwischenprodukt X
E — oder auch ein Protonenpool — gebildet werden, das bei dem Energietransfer vom Elektronentransportsystem zur ATP aufgebaut wird. Schließlich muß berücksichtigt werden, daß durch die Vorbelichtung an den Chloroplastenmembranen ein verstärkter ATP-Pi-Austausch zustande kommen könnte, der nach Belichtung nur langsam abklingt.
Abkürzungen ATP Adenosintriphosphat - ADP Adenosindiphosphat - Pi Orthophosphat - Poly-P anorganisches Polyphosphat - RNS Ribonucleinsäure - TCE Trichloressigsäure - 2,4-DNP 2,4-Dinitrophenol Stipendiat der Nishina-Gedächtnis-Stiftung (Japan) für 1963. 相似文献
Influence of preillumination on subsequent phosphorylation in the darkness of unicellular green algae (Ankistrodesmus braunii)
Summary Preilluminated unicellular green algae (Ankistrodesmus braunii) were treated in the subsequent darkness with 32PO4. The post-illumination dark incorporation was considerably increased compared with the control in continuous dark. The labeling of the separated phosphate-fractions was similar to that of continuous light. The light-induced dark incorporation depended from the light intensity as well as from the time of preillumination. A preillumination of 7 min was required for a maximal enhancement of this preillumination effect. On the other hand the effect diminished in darkness with a half life of approximately 4 min. Finally the enhancement was found to be greater in the absence of CO2 than in the presence of CO2.The experiments demonstrate the light-induced formation of a state in the algae, which permits the enhancement of 32P-incorporation into several phosphate-fractions for a limited time during subsequent darkness.It is discussed, that this may be performed through the formation of a light-reduced substance R maintaining for a limited time a cyclic electron transport in darkness, coupled with phosphorylation. On the other hand it seems possible, that preillumination induces a high energy intermediate X E—this could also be a pool of protons—formed in the course of energy-transfer from electron transport to ATP-formation. But we must consider also the possibility that light accellerates the ATP-Pi exchange on chloroplast-membranes for a time after preillumination.
Abkürzungen ATP Adenosintriphosphat - ADP Adenosindiphosphat - Pi Orthophosphat - Poly-P anorganisches Polyphosphat - RNS Ribonucleinsäure - TCE Trichloressigsäure - 2,4-DNP 2,4-Dinitrophenol Stipendiat der Nishina-Gedächtnis-Stiftung (Japan) für 1963. 相似文献
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