Cuticle ultrastructure of Hesionid polychaetes (Annelida)

Summary The structure of the cuticle in the four species of the family Hesionidae(Microphthalmus cf.listensis, M. cf.similis, Hesionides arenaria, juv.Podarke spec.) investigated basically corresponds to that found in all annelids. It consists of an outer, electron dense layer, epicuticle, basal cuticle with a fibrous layer, and numerous microvilli which penetrate the layers and are covered by a more or less dense glycocalyx. However, a rough collagen grid is not developed, the fibers are much thinner and are arranged in a more irregular manner. This corresponds to structures found in archiannelids and polychaete larvae. We consider them here to be reductions of the typical polychaete cuticle and postulate a correlation to the small body size of the species investigated. The quantitative differences in cuticle dimensions in the various body regions and structures can also be explained on a purely functional basis, especially apparent in the comparison of prostomium and body trunk. The pharynx cuticle shows significant structural differences due to the development of an additional peripherical lamellar layer-known to this extent only in gastrotrichs—as well as differently shaped and unusually long microvilli. This character is discussed as a possible synapomorphy for the family Hesionidae.

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Zusammenfassung Der Aufbau der Kutikula der 4 untersuchten Species aus der Familie Hesionidae(Microphthalmus cf.listensis, M. cf.similis, Hesionides arenaria, juv.Podarke spec.) entspricht grundsätzlich den Verhältnissen bei allen Anneliden: äußere elektronendichte Schicht, Epikutikula, basale Kutikula mit Faserschicht und zahlreiche Mikrovilli, die diese Schichten durchbrechen und von einem mehr oder weniger dichten Glykokalyx bedeckt sind. Ein derbes Kollagengitter ist jedoch nicht ausgebildet; die Fibrillen der Faserschicht sind wesentlich feiner und unregelmäßiger angeordnet. Dies entspricht Strukturen, wie sie bei Archianneliden und bei Polychaetenlarven gefunden werden. Wir deuten sie hier als Reduktionen der typischen Poly chaetenkutikula und vermuten eine Beziehung zur geringen Körpergröße der untersuchten Arten. Rein funktionell lassen sich auch die quantitativen Unterschiede in den verschiedenen Bereichen der Körperoberfläche deuten, die besonders im Vergleich von Prostomium und Rumpf zum Ausdruck kommen. Die Pharynxkutikula zeigt starke strukturelle Abweichungen durch die Ausbildung einer zusätzlichen peripheren Lamellenschicht (in diesem Ausmaß nur von den Gastrotrichen bekannt) und abweichend geformter, besonders langer Mikrovilli. Dieses Merkmal wird als mögliche Synapomorphie für die Familie Hesionidae diskutiert.

     

UV-induced allevation of lambda restriction in Escherichia coli K-12: kinetics of induction and specificity of this SOS function

Summary In UV-irradiated cells of Escherichia coli K-12 a partial release of the restriction of non-modified phage is observed when the cells are recA ⁺ lexA ⁺. We show here that the induction of this restriction allevation (RA) also depends on the recBC enzyme and that the expression of RA requires protein synthesis. Maximum expression was reached within 60 to 90 min after irradiation. Experiments are presented which show that upon UV-irradiation a signal is created which triggers the development of RA when protein synthesis is allowed. This signal decayed with a half-life of only a few minutes in cells treated with chloramphenicol. The decay kinetics were similar in uvr ⁺ and uvrA mutants. RA appeared to be specific for EcoK insofar as no allevation of restriction by EcoRI, EcoRII and EcoP1 occurred. During maximum expression of RA no gross reduction of the activities of the recBC enzyme (exonuclease V) and the restriction endonuclease EcoK was observed and no new DNA modifying activity appeared in the cells. Since, in fully expressed cells, up to 75% of the infecting DNA was converted to acid-soluble material within 20 min after infection we suggest that only a small specific fraction of infections may undergo RA.     

The coxal glands of geophilomorpha (chilopoda): Organs of osmoregulation

Summary The organs terminating at the coxal pores of the tug-legs of Geophilomorpha are not repugnatorial glands, but possess typical transport epithelia with deep apical and basal infoldings of the cell membranes, between which numerous large mitochondria are located. Many transport vesicles are found in the basal region but fewer in the apical cytoplasm. The apex is characterized by bundles of longitudinally oriented microtubules, sparse endoplasmic reticulum and free ribosomes. Single neurosecretory axons with synaptoid areas are scattered among the cells. It is suggested that the coxal organs have a diuretic function in moist habitats and an antidiuretic effect in arid environments. The switch-over is evidently controlled by a neuroendocrine mechanism.     

Heterochromatin of the Drosophila melanogaster Y chromosome as modifier of position effect variegation: the time of its action.

Summary Addition of heterochromatin suppresses while subtraction enhances position effect variegation. The heterochromatin-sensitive period has been determined in white/white-apricot variegated eyes of Y ^S w ^a /w ^a ; Dp (1;3) w ^265-58 flies. When such larvae, carrying a Y-short (Y ^S ) arm at the distal end of one X chromosome, are X-rayed, mitotic recombination leads to one daughter cell with two Y ^S arms and an adjacent daughter cell with no Y ^S arm. When induced after clonal initiation, the frequency of dark clones developing from daughter cells with two Y ^S arms is significantly higher than the frequency of dark clones in the rest of the eye; and this frequency is. even higher when induced before clonal initiation. The modifying action of the Y-heterochromatin is exerted, therefore, during and after clonal initiation. Surprisingly, the frequency of dark clones developing from cells with no Y ^S arm is not lower than the frequency of dark clones in the rest of the eye.     

Presence of hybridizing DNA sequences homologous to bovine acidic and basicβ-crystallins in all classes of vertebrates

Summary The eye lens-crystallins in cow and chicken are encoded by a family of at least six genes. In order to assess the distribution of the corresponding genes among other vertebrates we hybridized -crystallin sequences (A2, A3/A1, A4, B1, B2, B3), isolated from a bovine lens cDNA library, to Southern blots on whichEcoR1-digested chromosomal DNA was blotted from different vertebrate species. These included human, chimpanzee, calf, rat, pigeon, duck, monitor lizard, toad, trout, and lamprey. Positive hybridization signals were found in the representatives of virtually all classes of vertebrates. The basic B-crystallins gave hybridization signals in more species than the acidic A ones. In monitor lizard and toad the weakest hybridization signals for basic crystallin probes were found. For acidic crystallin probes the distribution pattern was more simple; among cold-blooded vertebrates a signal for A2 was found in trout and lamprey, for A4 in trout, and for A3/A1 only in toad. The results demonstrate that the duplications leading to the -crystallin gene family occurred before or during the earliest stages of vertebrate evolution.     

The hemocytes of the centipedeLithobius forficatus (Chilopoda,Lithobiomorpha)

Summary Five different types of hemocytes are found within the hemolymph ofLithobius forficatus: (1) small prohemocytes, (2) very actively spreading plasmatocytes, (3) granulocytes which have a lower spreading ability but tend to agglutinate, (4) spherulocytes which are filled with spherules, and (5) presumably, a coagulocyte, characterized by instant disintegration. Cystocytes as described forL. forficatus in the literature are preparation artifacts. Cell types are characterized by electron microscopy and in vitro and vital staining techniques at the light microscopic level. Results are discussed with reference to different nomenclatures and functions of hemocytes in other arthropods.     

MUTATION LOAD AND THE SURVIVAL OF SMALL POPULATIONS

Previous attempts to model the joint action of selection and mutation in finite populations have treated population size as being independent of the mutation load. However, the accumulation of deleterious mutations is expected to cause a gradual reduction in population size. Consequently, in small populations random genetic drift will progressively overpower selection making it easier to fix future mutations. This synergistic interaction, which we refer to as a mutational melt-down, ultimately leads to population extinction. For many conditions, the coefficient of variation of extinction time is less than 0.1, and for species that reproduce by binary fission, the expected extinction time is quite insensitive to population carrying capacity. These results are consistent with observations that many cultures of ciliated protozoans and vertebrate fibroblasts have characteristic extinction times. The model also predicts that clonal lineages are unlikely to survive more than 10⁴ to 10⁵ generations, which is consistent with existing data on parthenogenetic animals. Contrary to the usual view that Muller's ratchet does more damage when selection is weak, we show that the mean extinction time declines as mutations become more deleterious. Although very small sexual populations, such as self-fertilized lines, are subject to mutational meltdowns, recombination effectively eliminates the process when the effective population size exceeds a dozen or so. The concept of the effective mutation load is developed, and several procedures for estimating it are described. It is shown that this load can be reduced substantially when mutational effects are highly variable.     

Genetic control of human apolipoprotein E polymorphism: Comparison of one-and two-dimensional techniques of isoprotein analysis

Summary Genetic polymorphism of human apolipoprotein E (apo E) has previously been demonstrated by one-dimensional isoelectric focusing (Utermann et al. 1977b) and by two-dimensional electrophoresis of apolipoproteins (Zannis et al. 1981), but the relationship between the results obtained by these methods remained unclear. We therefore performed comparative phenotyping by one-dimensional and two-dimensional electrophoresis. Apoproteins from very low-density lipoproteins (apo VLDL) prepared by ultracentrifugation or from an apo Erich lipoprotein fraction prepared by heparin/Mg⁺⁺ precipitation, were used as a source of apo E. Six common phenotypes designated apo E-4/4, apo E-N/N, apo E-D/D, apo E-4/N, apo E-4/D, and apo E-N/D were differentiated irrespective of the technique used or the source of apolipoproteins, but the two-dimensional electrophoresis of apo VLDL and apo VLDL which had been treated with neuraminidase was the key for the correct genetic interpretation of those phenotypes exhibiting the E4 isoform of the protein. Each phenotype is characterized by the presence of either one or two of three major isoforms E2, E3, and E4 and by the presence of several minor sialylated forms of these proteins (apo E_s) that have higher apparent molecular weights. The unsialylated major isoform apo E2 does not only differ in charge but also has a higher apparent mol.wt. (about 34,500) than the major isoforms apo E3 and apo E4 (mol. wt. about 33,000). Family studies including 90 matings with a total of 203 offspring confirmed the genetic one locus model of Zannis et al. (1981). Apo E phenotypes are controlled by three autosomal codominant alleles apo E^d, apo Eⁿ, and apo E⁴ that specify for the E2, E3, and E4 isoforms respectively. Phenotypes apo E-D/D,-N/N, and-4/4 represent homozygotes and phenotypes apo E-4/N,-4/D, and-N/D heterozygotes for these alleles.The frequencies of apo E alleles in 1031 blood donors were apo E⁴=0.150, apo Eⁿ=0.773, and apo E^d=0.077. Homozygosity for the allele apo E^d is associated with hyperlipoproteinemia type III. Hence a large number of the population (about 1%) are at risk for this specific lipoprotein disorder that is associated with premature atherosclerosis and xanthomatosis.     

Effects of Detergents, Proteins, and Lipids on 2'':3''-Cyclic-Nucleotide 3''-Phosphodiesterase Activity

The myelin marker 2':3'-cyclic-nucleotide 34'-phosphodiesterase (CNPase) was isolated to a lipid- and phosphate-free stage. The effects of exogenously added lipids were tested on this preparation and compared to the known stimulation of the enzyme by detergents and proteins. CNPase could be stimulated 2-3 fold by these various agents which appeared to be additive in their effect. Enzyme-protein and enzyme-lipid interactions and possible medical use of the improved assay conditions for CNPase employed in the study are discussed.     

Internally elongated rodent α-crystallin A chain: Resulting from incomplete RNA splicing?

αA^Ins, an elongated α-crystallin A chain previously observed in rat, was present beside the normal αA chain in mouse, gerbil and hamster, which places its origin at least 30 million years ago. Like in rat the sequences of golden hamster αA^Ins and αA were found to be identical, apart from the internal insertion of 22 residues in αA^Ins. The hamster chains only differed from the rat chains by a single substitution in the inserted sequence of αA^Ins. The origin of αA^Ins, by insertion of 22 residues in an otherwise unchanged αA chain, and its rigid evolutionary conservation are most easily explained by assuming the incomplete removal of a putative intervening sequence from the precursor mRNA of αA, leaving an intracistronic insert of 66 nucleotides in part of the eventually translated mRNA.