全文获取类型
收费全文 | 440篇 |
免费 | 34篇 |
国内免费 | 1篇 |
专业分类
475篇 |
出版年
2022年 | 6篇 |
2021年 | 12篇 |
2020年 | 8篇 |
2019年 | 2篇 |
2018年 | 10篇 |
2017年 | 13篇 |
2016年 | 16篇 |
2015年 | 20篇 |
2014年 | 26篇 |
2013年 | 18篇 |
2012年 | 29篇 |
2011年 | 28篇 |
2010年 | 13篇 |
2009年 | 11篇 |
2008年 | 23篇 |
2007年 | 38篇 |
2006年 | 36篇 |
2005年 | 25篇 |
2004年 | 34篇 |
2003年 | 23篇 |
2002年 | 18篇 |
2001年 | 11篇 |
2000年 | 5篇 |
1999年 | 11篇 |
1998年 | 1篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1981年 | 2篇 |
1979年 | 1篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1970年 | 2篇 |
1969年 | 1篇 |
1967年 | 1篇 |
1966年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有475条查询结果,搜索用时 15 毫秒
61.
Competition between phytophagous insects could be mediated by differential impact of natural enemies or by induced changes in host plant quality. The interaction between the aphids Sipha flava and Rhopalosiphum maidis on the shared host plant, Sorghum halepense , was evaluated during the fall and spring seasons in the presence or absence of natural enemies in a garden experiment. During the fall, S. flava was negatively affected by R. maidis in all treatments. However, during the spring season, S. flava was not affected by R. maidis when natural enemies were excluded, and positively affected by R. maidis with natural enemies present. Rhopalosiphum maidis was negatively affected by S. flava during the fall and spring seasons under all treatments. Laboratory experiments demonstrated that changes in host plant quality induced by S. flava attack and the presence of the parasitoid Lysiphlebus testaceipes negatively affected R. maidis , but not S. flava . Under abiotic conditions simulating fall and spring (lower temperature and daylength in the fall than in the spring), S. flava showed lower performance than R. maidis under "fall" conditions, and both aphid species showed similar life history traits under "spring" conditions. For S. flava , the time to the first reproduction and longevity were longer, and the nymphal production was lower, under "fall" conditions than under "spring" conditions. Our results showed that S. flava is benefited when R. maidis and the parasitoid L. testaceipes are present but the abiotic conditions are not adverse. 相似文献
62.
Immunogenicity and safety assessment of the Cuban recombinant hepatitis B vaccine in healthy adults.
Zurina Cinza Estévez Arístides Aguilar Betancourt Verena Muzio González Nelvis Figueroa Baile Carmen Valenzuela Silva Francisco Hernández Bernal Eduardo Pentón Arias Aurora Delhanty Fernández Nelia Martin Olazábal Amaurys del Río Martín Lester Leal Batista Gloria Véliz Ríos Héctor Hernández Hernández Aracelis Blanco Hernández Evelyn Pérez Lugo Joel de la Torre Cruz Bertha L Batista Marchec Leovaldo Alvarez Falcón Jannet Trujillo Brito Darién Ortega León Pedro López Saura 《Biologicals》2007,35(2):115-122
Manufactures of biotechnological/biological products (including vaccines) frequently make changes to manufacturing processes of products both during development and after approval. In our case, a non-inferiority bridging study was carried out to demonstrate that changes in the production plant facilities of Cuban recombinant hepatitis B vaccine, Heberbiovac HB, did not affect the safety and immunogenicity of the vaccine. This controlled, randomized, doubled-blinded trial included 501 volunteers, aged between 20 and 64, who were given three doses of vaccine (20 microg HBsAg/mL) at month 0, 1, and 2. Four lots were evaluated (three corresponding to the new production facilities and a control one produced in the older facilities). One month after the third dose, were observed protective levels of anti-HBsAg in 97% of the subjects that concluded the study with a geometric mean antibody titer (GMT) of 931.18 IU/L. Normal values of body mass index (BMI), the younger ages, and being a female, were significantly related to a good antibody response. The vaccine was well tolerated. Pain at the injection site was the most commonly reported symptom. We conclude that Heberbiovac HB vaccine maintains its characteristics after the modifications carried out in the production plant facilities and both, lot obtained in previous facilities and in the new ones, are comparable in terms of safety and immunogenicity. 相似文献
63.
Jorge?A.?Falcón-Lezama Ruth?A.?Martínez-Vega Pablo?A.?Kuri-Morales José?Ramos-Casta?eda Ben?AdamsEmail author 《Bulletin of mathematical biology》2016,78(10):2011-2033
Dengue is a growing public health problem in tropical and subtropical cities. It is transmitted by mosquitoes, and the main strategy for epidemic prevention and control is insecticide fumigation. Effective management is, however, proving elusive. People’s day-to-day movement about the city is believed to be an important factor in the epidemiological dynamics. We use a simple model to examine the fundamental roles of broad demographic and spatial structures in epidemic initiation, growth and control. We show that the key factors are local dilution, characterised by the vector–host ratio, and spatial connectivity, characterised by the extent of habitually variable movement patterns. Epidemic risk in the population is driven by the demographic groups that frequent the areas with the highest vector–host ratio, even if they only spend some of their time there. Synchronisation of epidemic trajectories in different demographic groups is governed by the vector–host ratios to which they are exposed and the strength of connectivity. Strategies for epidemic prevention and management may be made more effective if they take into account the fluctuating landscape of transmission intensity associated with spatial heterogeneity in the vector–host ratio and people’s day-to-day movement patterns. 相似文献
64.
Roberta Nunes Horta Vivian Francilia Silva Kahl Merielen da Silva Sarmento Marisa Fernanda Silva Nunes Carem Rejane Maglione Porto Vanessa Moraes de Andrade Alexandre de Barros Falc?o Ferraz Juliana Da Silva 《Genetics and molecular biology》2016,39(1):122-128
Metal ions such as iron can induce DNA damage by inducing reactive oxygen species (ROS) and oxidative stress. Vitamin C is one of the most widely consumed antioxidants worldwide, present in many fruits and vegetables, especially inMalpighia glabra L., popularly known as acerola, native to Brazil. Acerola is considered a functional fruit due to its high antioxidant properties and phenolic contents, and therefore is consumed to prevent diseases or as adjuvant in treatment strategies. Here, the influence of ripe and unripe acerola juices on iron genotoxicity was analyzed in vivo using the comet assay and micronucleus test. The comet assay results showed that acerola juice exerted no genotoxic or antigenotoxic activity. Neither ripe nor unripe acerola juices were mutagenic to animals treated with juices, in micronucleus test. However, when compared to iron group, the pre-treatment with acerola juices exerted antimutagenic activity, decreasing significantly micronucleus mean values in bone marrow. Stage of ripeness did not influence the interaction of acerola compounds with DNA, and both ripe and unripe acerola juices exerted protective effect over DNA damage generated by iron. 相似文献
65.
Marinho C Alho I Arduíno D Falcão LM Brás-Nogueira J Bicho M 《Biochemical and biophysical research communications》2007,353(2):344-350
The aim of this study is to investigate GSTM1, GSTT1 and MTHFR genetic polymorphisms and its relation with total plasma glutathione (tGSH) levels in hypertension. Genotype distributions of GSTM1 and GSTT1 deletion polymorphisms and C677T variant of MTHFR were examined in a sample of 94 hypertensive patients with congestive heart failure and 207 healthy unrelated Portuguese individuals using PCR techniques. Plasma GST activity was determined spectrophotometrically. The antioxidant status was evaluated by fluorometric assays of tGSH. Genotype distributions of GSTT1 (chi2 test; p < 0.01) and MTHFR (chi2 test; p < 0.01) differ significantly between control and hypertensive patients with a greater prevalence of "non-null GSTT1/M1" and CT (heterozygous) genotypes. Moreover, GST activity and tGSH were markedly decreased in hypertension but there is no correlation with the studied polymorphisms. GSH depletion confirmed the possible involvement of oxidative stress in this pathology. Deletion of GSTT1 gene might be considered as protective factor for hypertension. 相似文献
66.
V. Regalado F. Perestelo A. Rodríguez A. Carnicero F. J. Sosa G. De la Fuente M. A. Falcón 《Applied microbiology and biotechnology》1999,51(3):388-390
Laccase, aryl-alcohol oxidase and superoxide radicals were detected in ligninolytic cultures of Fusarium proliferatum. Enzyme activities were present during the secondary metabolism and seen as single protein bands after non-denaturing electrophoresis.
In contrast, superoxide radicals were detected during primary growth, correlating with maximal lignin mineralization. Moreover,
ligninolysis decreased when scavengers of both superoxide and hydroxyl radicals were added to cultures, indicating that activated
oxygen species are involved in lignin degradation.
Received: 12 October 1998 / Accepted: 24 October 1998 相似文献
67.
Aims Foliar herbivory and water stress may affect floral traits attractive to pollinators. Plant genotypes may differ in their responses to the interplay between these factors, and evolution of phenotypic plasticity could be expected, particularly in heterogeneous environments. We aimed at evaluating the effects of simulated herbivory and experimental drought on floral traits attractive to pollinators in genetic families of the annual tarweed Madia sativa, which inhabits heterogeneous environments in terms of water availability, herbivore abundance and pollinator abundance.Methods In a greenhouse experiment with 15 inbred lines from a M. sativa population located in central Chile (Mediterranean-type climate), we measured the effects of apical bud damage and reduced water availability on: number of ray florets per flower head, length of ray florets, flower head diameter, number of open flower heads per plant, flowering plant height and flowering time.Important findings Apical damage and water shortage reduced phenotypic expression of floral traits attractive to pollinators via additive and non-additive effects. Plants in low water showed decreased height and had fewer and shorter ray florets, and fewer and smaller flower heads. Damaged plants showed delayed flowering, were less tall, and showed shorter ray florets and smaller flower heads. The number of ray florets was reduced by damage only in the low water treatment. Plant height, flowering time and number of flower heads showed among-family variation. These traits also showed genetic variation for plasticity to water availability. Ray floret length, flower head size and time to flowering showed genetic variation for plastic responses to apical damage. Plasticity in flowering time may allow M. sativa to adjust to the increased aridity foreseen for its habitat. Because genetic variation for plastic responses was detected, conditions are given for evolutionary responses to selective forces acting on plastic traits. We suggest that the evolution of adaptive floral plasticity in M. sativa in this ecological scenario (heterogeneous environments) would result from selective forces that include not only pollinators but also resource availability and herbivore damage. 相似文献
68.
Lilia Maria de Azevedo Moreira Acácia Fernandes Lacerda de Carvalho Ana Lúcia Vieira de Freitas Borja Paula Sanders Pereira Pinto Adriana Silveira Lucy Magalhães de Freitas Maria de Lourdes Lima Falcão 《Journal of applied genetics》2008,49(4):415-420
We report on the clinical observation of a girl patient with few signs of cri-du-chat syndrome. The chromosomal analysis in
lymphocyte culture showed 46,XX,del(5)(p15.3) in 38% of cells. Psychological tests revealed motor, perceptive and visual-spatial
problems, as well as immaturity and emotional dependence. The phoniatric evaluation showed poor vocabulary, difficulty with
repeating words or numbers in sequence, and better receptive than expressive language. The spectrographic measurements showed
disturbance of fundamental frequency (F0) in vocal pronunciation. The anatomic findings of the laryngoscopic evaluation were normal, indicating that the voice and
speech problems were functional disorders. The present case revealed moderate clinical signs and vocal disturbance associated
with a low percentage of 5p-cells and the breakpoint at 5p15.3. The short terminal deletion with a possible loss of the critical
region for cat-like cry and the presence of a normal cell line, explain the cry not so typical at birth (weak but not high-pitched),
the intermediate values of F0, and the moderate mental retardation. This case is compared with other mosaic 5p-patients reported in the literature. 相似文献
69.
Claudet J Osenberg CW Benedetti-Cecchi L Domenici P García-Charton JA Pérez-Ruzafa A Badalamenti F Bayle-Sempere J Brito A Bulleri F Culioli JM Dimech M Falcón JM Guala I Milazzo M Sánchez-Meca J Somerfield PJ Stobart B Vandeperre F Valle C Planes S 《Ecology letters》2008,11(5):481-489
Marine reserves are widely used throughout the world to prevent overfishing and conserve biodiversity, but uncertainties remain about their optimal design. The effects of marine reserves are heterogeneous. Despite theoretical findings, empirical studies have previously found no effect of size on the effectiveness of marine reserves in protecting commercial fish stocks. Using 58 datasets from 19 European marine reserves, we show that reserve size and age do matter: Increasing the size of the no-take zone increases the density of commercial fishes within the reserve compared with outside; whereas the size of the buffer zone has the opposite effect. Moreover, positive effects of marine reserve on commercial fish species and species richness are linked to the time elapsed since the establishment of the protection scheme. The reserve size-dependency of the response to protection has strong implications for the spatial management of coastal areas because marine reserves are used for spatial zoning. 相似文献
70.
Metabolic symbiosis and the birth of the plant kingdom 总被引:2,自引:0,他引:2
Deschamps P Colleoni C Nakamura Y Suzuki E Putaux JL Buléon A Haebel S Ritte G Steup M Falcón LI Moreira D Löffelhardt W Raj JN Plancke C d'Hulst C Dauvillée D Ball S 《Molecular biology and evolution》2008,25(3):536-548
Eukaryotic cells are composed of a variety of membrane-bound organelles that are thought to derive from symbiotic associations involving bacteria, archaea, or other eukaryotes. In addition to acquiring the plastid, all Archaeplastida and some of their endosymbiotic derivatives can be distinguished from other organisms by the fact that they accumulate starch, a semicrystalline-storage polysaccharide distantly related to glycogen and never found elsewhere. We now provide the first evidence for the existence of starch in a particular species of single-cell diazotrophic cyanobacterium. We provide evidence for the existence in the eukaryotic host cell at the time of primary endosymbiosis of an uridine diphosphoglucose (UDP-glucose)-based pathway similar to that characterized in amoebas. Because of the monophyletic origin of plants, we can define the genetic makeup of the Archaeplastida ancestor with respect to storage polysaccharide metabolism. The most likely enzyme-partitioning scenario between the plastid's ancestor and its eukaryotic host immediately suggests the precise nature of the ancient metabolic symbiotic relationship. The latter consisted in the export of adenosine diphosphoglucose (ADP-glucose) from the cyanobiont in exchange for the import of reduced nitrogen from the host. We further speculate that the monophyletic origin of plastids may lie in an organism with close relatedness to present-day group V cyanobacteria. 相似文献