A new method for obtaining bacteria-free cultures of blue-green algae

Blue-green algae are enriched and subcultured in a nitrogen-free medium. Portions of these cultures are kept at 47–48 C for 15, 30, 45 and more min. If akinetes are present and spore-forming bacteria absent — as is often the case — bacteria-free cultures of the algae are obtained.     

Demonstration of X chromatin in drumstick-like nuclear appendages of leukocytes by in situ hybridization on blood smears

Summary An X chromosome specific nucleic acid probe was used to study the positions of the X chromosomes in leukocyte nuclei by in situ hybridization to smears of peripheral blood. This autoradiographic approach allowed the first direct demonstration of the presence of X chromosomal material in the drumstick-like structures of female polymorphonuclear leukocytes.In honour of Prof. P. van Duijn