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排序方式: 共有96条查询结果,搜索用时 15 毫秒
41.
Circulating intercellular adhesion molecules in blood and bronchoalveolar lavage in Behçet's disease
The aim of this study was to evaluate circulating intercellular adhesion molecule-1 (cICAM-1) in serum and in bronchoalveolar lavage (BAL), as a marker for the inflammatory process in patients with active Behçet''s disease (BD). Circulating ICAM-1 was tested by an enzyme linked immuno-sorbent assay in serum and in BAL of patients with BD. These values were compared to those of patients with tuberculosis and to healthy controls. Increased levels of circulating ICAM-1 were found in serum from patients with active BD compared to healthy controls (p < 0.01). Similar levels of serum cICAM-1 were found in BD and tuberculosis. Additionally, both BD and tuberculosis patients exhibited high levels of cICAM-1 in BAL fluid, suggesting that this increase may be a result of the immune system activation in inflammatory sites. Circulating ICAM-1 seemed to have a good discriminative power in identifying active BD, being elevated in all active stages (p < 0.01) compared to remission BD stage. No differences were found in active BD patients depending upon the clinical manifestations. These results suggest that cICAM-1 may be involved in leucocyte adhesion and migration into the vessel wall of the lung. Circulating forms are derived from molecules expressed on the surface of activated cells, as a result of an inflammatory process. 相似文献
42.
Ferjani Zouidi Mouna Stayoussef Dorra Bouzid Hajer Fourati Olfa Abida Costa João Mourad Ben Ayed Raouia Fakhfakh Kammoun Thouraya Hachicha Monjia Penha-Gonçalves Carlos Hatem Masmoudi 《Gene》2014
Type 1 diabetes (T1D) is an autoimmune disease (AID) with both genetic and environmental components. We aimed to investigate the genetic association of polymorphisms in genes previously linked with other AIDs, namely BANK1, IL15 and IL2/IL21 region. 相似文献
43.
Fathia Aouidi Hana Gannoun Nada Ben Othman Lamia Ayed Moktar Hamdi 《Process Biochemistry》2009,44(5):597-601
The effect of the cheese whey's (CW) addition on the fermentative decolorization of olive mill wastewater (OMW) by Lactobacillus paracasei, with and without pH adjustment by lime, was investigated. Mixtures OMW/CW at different proportions were fermented. The highest colour removal (47%) and total phenolic reduction (22.7%) of OMW were obtained after cofermentation of OMW/CW at proportions of 10/90, respectively. The decrease of pH after cofermentation of the two wastewaters, induced the precipitation of whey proteins with phenolic compounds and, so, improves decolorization. These removal yields reached 64% and 34%, respectively after precipitation by adjustment of pH at 7 with lime at the end of cofermentation. These improvements were correlated to a clarification of wastewaters by precipitation of whey proteins with phenolic compounds. An enhanced decolorization (up to 93%) and a total phenolic reduction (50%) of the mixture were obtained when cofermentation sequentially pH corrected by lime addition was investigated. 相似文献
44.
The coupling of glycals with an alkynyl iodide bearing a protected amino alcohol moiety was achieved in the presence of metallic indium under Barbier conditions. It gave functionalized C-glycosyl compounds, precursors of C-glycosyl amino acids with α configuration. 相似文献
45.
Effects of form of olive cake (OC) on growth performances and carcass quality were studied on forty-eight Awassi lambs. All lambs were male with an average body weight of 29.5 kg (S.D. = 2.3 kg) at the beginning of the experiment. Animals were randomly divided into four groups of 12 lambs each. Lambs in each group received individually corn–soybean meal (SBM) total mixed rations (TMRs). Rations were incorporated with a fixed amount (149 g/kg DM) of OC of different forms: crude OC, a product of the three centrifugation extraction procedure (control group), alkali treated, ensiled and pelleted. All rations were isonitrogenous and isocaloric. The growth experiment lasted 10 weeks. In the following week, all lambs were slaughtered. At termination of the experiment, lambs fed crude, alkali treated or ensiled OC rations consumed more feed and gained more weight (P<0.05) than those fed the pelleted OC. This same trend was found for the feed conversion (FC), carcass and empty body weights (EBWs). However, external (hide, head and feet, HHF) and thoracic organs (heart and lungs, HL), gut and liver weights proportional to EBW (g/kg) were not affected by the form of OC. The form of OC had no effects on muscle (P=0.4) and bone (P=0.21) tissues. Carcass, pelvic, kidney fats and total carcass fats weights when expressed as g/kg EBW and the percent of carcass fat of total body fat (TBF) were lower in lambs fed the pelleted OC compared to those offered the other forms of OC. However, the subcutaneous, inter muscular and TBF fats weights (g/kg) were comparable among lambs in different OC form rations. Results from this work suggest that the treated OC had no advantages compared to crude in regard to parameters investigated in this research. 相似文献
46.
Lamia Ayed Kamel Chaieb Abdelkarim Cheref Amina Bakhrouf 《World journal of microbiology & biotechnology》2009,25(4):705-711
Triphenylmethane dyes belong to the most important group of synthetic colorants and are used extensively in the textile industries
for dying cotton, wool, silk, nylon, etc. They are generally considered as the xenobiotic compounds, which are very recalcitrant
to biodegradation. Sphingomonas paucimobilis, was isolated from the soil sample collected from contaminated sites of textile industry located in KsarHellal, Tunisia,
and it was able to decolorize Malachite Green (MG) dye (50 mg/l) within 4 h under shaking condition (pH 9 and temperature
25°C). The effect of inoculum size, dye concentration, temperature and initial pH of the solution were studied. The results
obtained from the batch experiments revealed the ability of the tested bacteria to remove dye. UV–Vis spectroscopy and FTIR
analysis of samples before and after decolorization confirmed the ability of the tested strain to decolorize MG. In addition,
the phytotoxicity study revealed the degradation of MG into non-toxic product by S. paucimobilis. 相似文献
47.
Rayda Ben Ayed Karim Ennouri Fathi Ben Amar Fabienne Moreau Mohamed Ali Triki Ahmed Rebai 《Biochemical genetics》2017,55(4):300-313
To enhance table olive tree authentication, relationship, and productivity, we consider the analysis of 18 worldwide table olive cultivars (Olea europaea L.) based on morphological, biological, and physicochemical markers analyzed by bioinformatic and biostatistic tools. Accordingly, we assess the relationships between the studied varieties, on the one hand, and the potential productivity–quantitative parameter links on the other hand. The bioinformatic analysis based on the graphical representation of the matrix of Euclidean distances, the principal components analysis, unweighted pair group method with arithmetic mean, and principal coordinate analysis (PCoA) revealed three major clusters which were not correlated with the geographic origin. The statistical analysis based on Kendall’s and Spearman correlation coefficients suggests two highly significant associations with both fruit color and pollinization and the productivity character. These results are confirmed by the multiple linear regression prediction models. In fact, based on the coefficient of determination (R 2) value, the best model demonstrated the power of the pollinization on the tree productivity (R 2 = 0.846). Moreover, the derived directed acyclic graph showed that only two direct influences are detected: effect of tolerance on fruit and stone symmetry on side and effect of tolerance on stone form and oil content on the other side. This work provides better understanding of the diversity available in worldwide table olive cultivars and supplies an important contribution for olive breeding and authenticity. 相似文献
48.
Identification and natural functions of cyclic lipopeptides from Bacillus amyloliquefaciens An6
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Hanen Ben Ayed Noomen Hmidet Max Béchet Philippe Jacques Moncef Nasri 《Engineering in Life Science》2017,17(5):536-544
Lipopeptides constitute a structurally diverse group of metabolites produced by various bacterial and fungal genera. In the past decades, research on lipopeptides has been fueled by their surfactant activities. However, natural functions of lipopeptides compounds have received considerably less attention. The aim of this study was to isolate and identify the lipopeptides from Bacillus amyloliquefaciens An6, and further evaluate their biological activities. An6 lipopeptides were detected by PCR using degenerated primers and MALDI‐TOF‐MS. An6 strain was found to produce surfactin, fengycin, and bacillomycin. Following their purification, the in vitro antioxidant activity of An6 lipopeptides was studied through different assays. The scavenging effect on 1,1‐diphenyl‐2‐picrylhydrazyl radicals at a dosage of 0.75 mg/mL was 81%. Its reducing power was concentration‐dependant and reached a maximum of 1.07 at 2.5 mg/mL. Moreover, they showed a strong inhibition of β‐carotene bleaching. An6 lipopeptides mixture was also found to display significant antimicrobial activity against several Gram‐positive, Gram‐negative bacteria, and fungal strains. An6 lipopeptides were insensitive to proteolytic enzymes, stable between pH 4.0 and 12.0, and resistant to high temperature. Our results provided enough evidence proving that An6 lipopeptides could be used as functional‐food components. 相似文献
49.
Latent and active p53 are identical in conformation 总被引:6,自引:0,他引:6
50.
Examination of sputum provides a direct method to investigate airway inflammation non-invasively in particular Th1 (IL-2, IFN-gamma) and Th2 (IL-4, IL-10) cytokine production. IL-2, IL-4, IL-10 and IFN-gamma cytokine were studied in induced sputum mononuclear cells of asthmatic patients. Sputum induction was performed on 10 patients and 10 normal controls. Basal and mitogen-stimulated cytokine production was determined in induced sputum T-cell culture. Supernatants were collected and assayed not only with specific ELISA but also with polymerase chain reaction (PCR) techniques. Data showed a significantly higher production of IL-10 by both the ELISA and the RT-PCR techniques in asthmatic patients compared with sputum mononuclear cells from healthy controls. IL-4 production was detected at a low level using the ELISA method in asthmatic patients. The RT-PCR analysis detected a significantly IL-4-mRNA expression in all asthmatic patients, compared with controls. Results of IL-10 and IL-4 mRNA expression were reproducible. We did not find any alteration in the expression of the type 1 derived cytokines (IL-2 and IFN-gamma) in asthmatic patients or in healthy controls. Our study showed a tendency of induced sputum mononuclear cells to express a Th2-like cytokine pattern in acute exacerbation of asthmatic patients, where IL-10 and IL-4 are synthesized in larger amounts. The combination of sputum induction as a non-invasive tool to explore the lung and the identification of disease-associated cytokine expression and of specific cytokine mRNA should help elucidate mechanisms of the immunologically mediated inflammatory responses in asthma. 相似文献