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141.
142.
Nikolaos E. Ioannidis Wiebke Zschiesche Olaf Barth Christos Kotakis Eleni Navakoudis Klaus Humbeck Kiriakos Kotzabasis 《Journal of Plant Growth Regulation》2014,33(1):77-90
Polyamines (PAs) are ubiquitous aliphatic amines important and, in many cases, essential for plant growth, abiotic stress response, and tolerance. Here we provide evidence for genetic reprogramming of PA homeostasis that occurs during the de-etiolation, maturation, and senescence of the primary leaf in Hordeum vulgare (barley). We analyzed expression levels of key genes in the anabolic and catabolic branches of PA metabolism throughout the life cycle of etioplasts, at all steps of the functional assembly of the photosynthetic apparatus, and during leaf senescence. The changes in the total PAs titer of the leaf were followed throughout the different developmental stages. Furthermore, we align all three stages of the photosynthetic performance (rapid light-dependent de-etiolation, phase of optimal efficiency, and senescence-induced deterioration) with the changes in PA homeostasis. Finally, we focus on two phases during aging (early and late senescence) and we present their bioenergetic (for example, PSII maximal efficiency, ATPase conductivity) and genetic profiles, with emphasis on sensitive parameters that describe this process for the photosynthetic apparatus and PA metabolism, respectively. In conclusion, the fine tuning of PA homeostasis is regulated by the simultaneous genetic reprogramming of the anabolic and catabolic branches of PA metabolism and adjusts all the developmental changes from de-etiolation to maturation and senescence. 相似文献
143.
Mesoamerican biodiversity is increasingly threatened by anthropogenic destruction of natural land cover. Habitat degradation and climate change are primary threats to specialized forest odonate species that are important model organisms for forest health and defining conservation units. The extreme niche specialization of Megaloprepus caerulatus, the world’s largest extant odonate, makes it well suited as an indicator for changing environmental conditions. Megaloprepus, which is considered to be a monospecific genus, is highly dependent on old growth forests whose water filled tree holes are limiting reproductive resources for this species. Here, we focus on the question how historical and recent fragmentation events, strong niche conservatism and ecological conditions have affected population dynamics, viability and the species status in this evolutionarily old genus. Two mitochondrial sequence markers (ND1 and 16S rRNA) and a set of microsatellites were used to analyze population structure and genetic diversity of M. caerulatus in the northern part of its distributional range. Results suggested an absence of gene flow and no shared haplotypes among the study populations. Statistical parsimony indicated high sub-structuring among populations with sequence diversity similar to levels found at the species level compared to other odonates. In sum, the genetic data suggest that Megaloprepus may actually consist of more than one species. The taxonomic status of the group should be revised in light of the three distinct genetic clusters found in different forest regions. The results may also allow insights into the impact of recent and historical habitat fragmentation on a strong Neotropical forest restricted insect species. 相似文献
144.
Burkhard Greve Kristin Dreffke Astrid Rickinger Stefan Könemann Eberhard Fritz Friederike Eckardt-Schupp Susanne Amler Cristina Sauerland Herbert Braselmann Wiebke Sauter Thomas Illig Peter Schmezer Maria Gomolka Normann Willich Tobias Bölling 《Apoptosis : an international journal on programmed cell death》2009,14(2):226-235
In the present study, the predictive value of ionising radiation (IR)-induced cell death was tested in peripheral blood lymphocytes
(PBLs) and their corresponding Epstein-Barr virus-transformed lymphoblastoid cell lines (LCLs) in an interlaboratory comparison.
PBLs and their corresponding LCLs were derived from 15 tumour patients, that were considered clinically radiosensitive based
on acute side-effects, and matched controls. Upon coding of the samples, radiosensitivity of the matched pairs was analysed
in parallel in three different laboratories by assessing radiation-induced apoptotic and necrotic cell death using annexin
V. All participating laboratories detected a dose-dependent increase of apoptosis and necrosis in the individual samples,
to a very similar extent. However, comparing the mean values of apoptotic and necrotic levels derived from PBLs of the radiosensitive
cohort with the mean values of the control cohort did not reveal a significant difference. Furthermore, within 15 matched
pairs, no sample was unambiguously and independently identified by all three participating laboratories to demonstrate in
vitro hypersensitivity that matched the clinical hypersensitivity. As has been reported previously, apoptotic and necrotic
cell death is barely detectable in immortalised LCL derivatives using low doses of IR. Concomitantly, the differences in apoptosis
or necrosis levels found in primary cells of different individuals were not observed in the corresponding LCL derivatives.
All participating laboratories concordantly reasoned that, with the methods applied here, IR-induced cell death in PBLs is
unsuitable to unequivocally predict the individual clinical radiosensitivity of cancer patients. Furthermore, LCLs do not
reflect the physiological properties of the corresponding primary blood lymphocytes with regard to IR-induced cell death.
Their value to predict clinical radiosensitivity is thus highly questionable.
The authors B. Greve, K. Dreffke and A. Rickinger are treated as first authors. 相似文献
145.
The effects of bioturbation in marine sediments are mainly associated with an increase in oxic and oxidized zones through an influx of oxygen‐rich water deeper into the sediment and the rapid transport of particles between oxic and anoxic conditions. However, macrofaunal activity also can increase the occurrence of reduced microniches and anaerobic processes, such as sulfate reduction. Our goal was to determine the two‐dimensional distribution of microniches associated with burrows of a ghost shrimp (Neotrypaea californiensis) and to determine microbial activities. In laboratory experiments, detailed measurements of sulfate reduction rates (SRR) were measured by injecting, in a 1 cm grid, radiolabelled sulfate directly into a narrow aquarium (40 cm × 30 cm × 3 cm) containing the complex burrow of an actively burrowing shrimp. Light‐coloured oxidized burrow walls, along with black reduced microniches, were clearly visible through the aquarium walls. Direct injection of radiotracers allowed for whole‐aquarium incubation to obtain two‐dimensional documentation of sulfate reduction. Results indicated SRR were up to three orders of magnitude higher (140–790 nmol SO42? cm?3 day?1) in reduced microniches associated with burrows when compared with the surrounding sediment. Additionally, some of the subsurface sulfate‐reducing microniches associated with the burrow system appeared to be zones of dinitrogen fixation. Bioturbation may also lead to decreased sulfate reduction in other microniches and the sum of the activity in all microniches might not result in a total increase of sulfate reduction compared with non‐bioturbated control sediments. 相似文献
146.
Tim Newbold Tom Reader Ahmed El‐Gabbas Wiebke Berg Wael M. Shohdi Samy Zalat Sherif Baha El Din Francis Gilbert 《Oikos》2010,119(8):1326-1334
Species distribution models are a very popular tool in ecology and biogeography and have great potential to help direct conservation efforts. Models are traditionally tested by using half the original species records to build the model and half to evaluate it. However, this can lead to overly optimistic estimates of model accuracy, particularly when there are systematic biases in the data. It is better to evaluate models using independent data. This study used independent species records from a new to survey to provide a more rigorous evaluation of distribution‐model accuracy. Distribution models were built for reptile, amphibian, butterfly and mammal species. The accuracy of these models was evaluated using the traditional approach of partitioning the original species records into model‐building and model‐evaluating datasets, and using independent records collected during a new field survey of 21 previously unvisited sites in diverse habitat types. We tested whether variation in distribution‐model accuracy among species could be explained by species detectability, range size, number of records used to build the models, and body size. Estimates of accuracy derived using the new species records correlated positively with estimates generated using the traditional data‐partitioning approach, but were on average 22% lower. Model accuracy was negatively related to range size and number of records used to build the models, and positively related to the body size of butterflies. There was no clear relationship between species detectability and model accuracy. The field data generally validated the species distribution models. However, there was considerable variation in model accuracy among species, some of which could be explained by the characteristics of species. 相似文献
147.
148.
Rodriguez D Bárcena M Möbius W Schleich S Esteban M Geerts WJ Koster AJ Griffiths G Locker JK 《Cellular microbiology》2006,8(3):427-437
The assembly of the intracellular mature virus (IMV) of vaccinia virus (VV), the prototype member of the poxviridae, is poorly understood and controversial. We have previously proposed that the IMV is composed of a continuous double-membraned cisterna derived from the smooth ER, whereby the genome-containing core is enwrapped by a part of this cisterna. In the present study we characterize a mutant virus in which the synthesis of the major core protein A10L can be conditionally expressed. Without A10L, IMVs are not made; immature viruses (IVs) and regularly stacked membrane structures that contain viral DNA, accumulate instead. By immunolabelling of thawed cryo-sections these stacks contain most of the viral core proteins and low levels of viral membrane proteins. Importantly, the stacked membranes could be labelled with antibodies to an ER marker protein, implying that they are derived from this cellular compartment. By electron tomography (ET) on semi-thin cryo-sections we show that the membranes of the stacks are continuous with the membranes of the IVs. Direct continuities with ER cisternae, to which the stacks are tightly apposed, were, however, not unequivocally seen. Finally, ET revealed how the IV membranes separated to become two-membrane profiles. Taken together, this study shows that VV core proteins and the viral DNA can coassemble onto ER-derived membranes that are continuous with the membranes of the IVs. 相似文献
149.