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Christoph Böttcher Andrea Krähmer Melanie Stürtz Sabine Widder Hartwig Schulz 《Metabolomics : Official journal of the Metabolomic Society》2017,13(4):35
Introduction
Onion (Allium cepa) represents one of the most important horticultural crops and is used as food, spice and medicinal plant almost worldwide. Onion bulbs accumulate a broad range of primary and secondary metabolites which impact nutritional, sensory and technological properties.Objectives
To complement existing analytical methods targeting individual compound classes this work aimed at the development and validation of an analytical workflow for comprehensive metabolite profiling of onion bulbs.Method
Metabolite profiling was performed by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (LC/ESI-QTOFMS). For annotation of metabolites accurate mass tandem mass spectrometry experiments were carried out.Results
On the basis of LC/ESI-QTOFMS and two chromatographic methods an analytical workflow was developed which facilitates profiling of polar and semi-polar onion metabolites including fructooligosaccharides, proteinogenic amino acids, peptides, S-substituted cysteine conjugates, flavonoids and saponins. To minimize enzymatic conversion of S-alk(en)ylcysteine sulfoxides, a sample preparation and extraction protocol for fresh onions was developed comprising cryohomogenization and a low-temperature quenching step. A total of 123 metabolites were annotated and characterized by chromatographic and tandem mass spectral data. For validation, recovery rates and matrix effects were determined for 15 model compounds. Repeatability and linearity were assessed for more than 80 endogenous metabolites.Conclusion
As exemplarily demonstrated by comparative metabolic analysis of six onion cultivars the established analytical workflow in combination with targeted and non-targeted data analysis strategies can be successfully applied for comprehensive metabolite profiling of onion bulbs.22.
Using higher organisms in biological early warning systems for real-time toxicity detection. 总被引:3,自引:0,他引:3
W H van der Schalie T R Shedd P L Knechtges M W Widder 《Biosensors & bioelectronics》2001,16(7-8):457-465
Many biological early warning systems (BEWS) have been developed in recent years that evaluate the physiological and behavioral responses of whole organisms to water quality. Using a fish ventilatory monitoring system developed at the US Army Centre for Environmental Health Research as an example, we illustrate the operation of a BEWS at a groundwater treatment facility. During a recent 12-month period, the fish ventilatory system was operational for 99% of the time that the treatment facility was on-line. Effluent-exposed fish responded as a group about 2.8% of the time. While some events were due to equipment problems or non-toxic water quality variations, the fish system did indicate effluent anomalies that were subsequently identified and corrected. The fish monitoring BEWS increased treatment facility engineers' awareness of effluent quality and provided an extra measure of assurance to regulators and the public. Many operational and practical considerations for whole organism BEWS are similar to those for cell- or tissue-based biosensors. An effective biomonitoring system may need to integrate the responses of several biological and chemical sensors to achieve desired operational goals. Future development of an 'electronic canary', analogous to the original canary in the coal mine, could draw upon advances in signal processing and communication to establish a network of sensors in a watershed and to provide useful real-time information on water quality. 相似文献
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Nonivamide Enhances miRNA let‐7d Expression and Decreases Adipogenesis PPARγ Expression in 3T3‐L1 Cells
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Field studies on the characteristics of light that influencevertical migrations in the mesopelagic realm are sparse, dueto the difficulty in simultaneously monitoring changes in speciesdistributions with changes in downwelling irradiance. Usingthe Johnson-Sea-Link submersible as a platform, in situ measurementsof the changes in downwelling irradiance at sunset were madesimultaneously with observations on changes in animal distributionpatterns in Wilkinson Basin, Gulf of Maine. The results indicatethat the vertical migrations for several species of large zooplanktonare staggered, with euphausiids (Meganyctiphanes norvegica)migrating first, cydippid ctenophores (Euplokamus) migratingnext, and two species of caridean shrimp (Dichelopandalus lepiocerusand Pasiphaea multidentata) migrating last Data collected ondaytime dives indicate that the daytime depth distribution isnot solely responsible for the migration order, and that differentspecies may be responding to different cues, or have differentthresholds for the same cue. 相似文献
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Sabine Kossmann Hanhan Hu Sebastian Steven Tanja Sch?nfelder Daniela Fraccarollo Yuliya Mikhed Melanie Br?hler Maike Knorr Moritz Brandt Susanne H. Karbach Christian Becker Matthias Oelze Johann Bauersachs Julian Widder Thomas Münzel Andreas Daiber Philip Wenzel 《The Journal of biological chemistry》2014,289(40):27540-27550
Endothelial nitric-oxide synthase (eNOS) uncoupling and increased inducible NOS (iNOS) activity amplify vascular oxidative stress. The role of inflammatory myelomonocytic cells as mediators of these processes and their impact on tetrahydrobiopterin availability and function have not yet been defined. Angiotensin II (ATII, 1 mg/kg/day for 7 days) increased Ly6Chigh and CD11b+/iNOShigh leukocytes and up-regulated levels of eNOS glutathionylation in aortas of C57BL/6 mice. Vascular iNOS-dependent NO formation was increased, whereas eNOS-dependent NO formation was decreased in aortas of ATII-infused mice as assessed by electron paramagnetic resonance (EPR) spectroscopy. Diphtheria toxin-mediated ablation of lysozyme M-positive (LysM+) monocytes in ATII-infused LysMiDTR transgenic mice prevented eNOS glutathionylation and eNOS-derived Nω-nitro-l-arginine methyl ester-sensitive superoxide formation in the endothelial layer. ATII increased vascular guanosine triphosphate cyclohydrolase I expression and biopterin synthesis in parallel, which was reduced in monocyte-depleted LysMiDTR mice. Vascular tetrahydrobiopterin was increased by ATII infusion but was even higher in monocyte-depleted ATII-infused mice, which was paralleled by a strong up-regulation of dihydrofolate reductase expression. EPR spectroscopy revealed that both vascular iNOS- and eNOS-dependent NO formation were normalized in ATII-infused mice following monocyte depletion. Additionally, deletion as well as pharmacologic inhibition of iNOS prevented ATII-induced endothelial dysfunction. In summary, ATII induces an inflammatory cell-dependent increase of iNOS, guanosine triphosphate cyclohydrolase I, tetrahydrobiopterin, NO formation, and nitro-oxidative stress as well as eNOS uncoupling in the vessel wall, which can be prevented by ablation of LysM+ monocytes. 相似文献
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The SBML ODE Solver Library: a native API for symbolic and fast numerical analysis of reaction networks 总被引:1,自引:0,他引:1
Machné R Finney A Müller S Lu J Widder S Flamm C 《Bioinformatics (Oxford, England)》2006,22(11):1406-1407
The SBML ODE Solver Library (SOSlib) is a programming library for symbolic and numerical analysis of chemical reaction network models encoded in the Systems Biology Markup Language (SBML). It is written in ISO C and distributed under the open source LGPL license. The package employs libSBML structures for formula representation and associated functions to construct a system of ordinary differential equations, their Jacobian matrix and other derivatives. SUNDIALS' CVODES is incorporated for numerical integration and sensitivity analysis. Preliminary benchmarking results give a rough overview on the behavior of different tools and are discussed in the Supplementary Material. The native application program interface provides fine-grained interfaces to all internal data structures, symbolic operations and numerical routines, enabling the construction of very efficient analytic applications and hybrid or multi-scale solvers with interfaces to SBML and non SBML data sources. Optional modules based on XMGrace and Graphviz allow quick inspection of structure and dynamics. 相似文献
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Early determinants of H2O2-induced endothelial dysfunction 总被引:4,自引:0,他引:4
Boulden BM Widder JD Allen JC Smith DA Al-Baldawi RN Harrison DG Dikalov SI Jo H Dudley SC 《Free radical biology & medicine》2006,41(5):810-817
Reactive oxygen species (ROS) can stimulate nitric oxide (NO(*)) production from the endothelium by transient activation of endothelial nitric oxide synthase (eNOS). With continued or repeated exposure, NO(*) production is reduced, however. We investigated the early determinants of this decrease in NO(*) production. Following an initial H(2)O(2) exposure, endothelial cells responded by increasing NO(*) production measured electrochemically. NO(*) concentrations peaked by 10 min with a slow reduction over 30 min. The decrease in NO(*) at 30 min was associated with a 2.7-fold increase in O(2)(*-) production (p < 0.05) and a 14-fold reduction of the eNOS cofactor, tetrahydrobiopterin (BH(4), p < 0.05). Used as a probe for endothelial dysfunction, the integrated NO(*) production over 30 min upon repeated H(2)O(2) exposure was attenuated by 2.1-fold (p = 0.03). Endothelial dysfunction could be prevented by BH(4) cofactor supplementation, by scavenging O(2)(*-) or peroxynitrite (ONOO(-)), or by inhibiting the NADPH oxidase. Hydroxyl radical (()OH) scavenging did not have an effect. In summary, early H(2)O(2)-induced endothelial dysfunction was associated with a decreased BH(4) level and increased O(2)(*-) production. Dysfunction required O(2)(*-), ONOO(-), or a functional NADPH oxidase. Repeated activation of the NADPH oxidase by ROS may act as a feed forward system to promote endothelial dysfunction. 相似文献
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Bioluminescence of sound-scattering layers in the Gulf of Maine 总被引:2,自引:0,他引:2
Submersible-based investigations of bioluminescence were conductedin sound-scattering layers (SSLs) in the Gulf of Maine, usingintensified video and dual-beam acoustic methods. Stimulatedbioluminescence in the SSLs was high (341 µW sr1m3 while spontaneous bioluminescence was not detected.The average intensity of individual bioluminescent sources inthe SSLs was 30200 times greater than the intensity oflight emitters outside the SSLs. The two brightest sources ofbioluminescence were identified as the euphausiid, Meganyctiphanesnorvegica and the cydippid ctenophore, Euplokamis sp. Meganyctiphanesnorvegica formed a SSL within 50 m of the bottom during theday and migrated to the uppermost 30 m of the water column atnight, forming a near-surface SSL. Euplokamis sp., which producedexceptionally intense and long-lasting bioluminescent secretions,occurred within the near-bottom SSL in concentrations up to7 m3. Our findings indicate that traditional methodsof identifying the primary light emitters in a region, basedon light measurements from net- or pump-captured organisms,may have underestimated the significant in situ bioluminescencepotential of euphausiids and gelatinous zooplankton.
3 Present address: NOAA/NURP/R-OR2, Silver Spring, MD 20910,USA 相似文献