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Zusammenfassung 19 Patienten, die nach einer Nierentransplantation mit Imuran behandelt wurden, wiesen eine erhöhte Anzahl Chromosomenabnormitäten im Vergleich zu 30 gesunden Kontrollpersonen der gleichen Altersklasse auf. Keine signifikant erhöhte Anzahl an Chromosomenabnormitäten fand sich jedoch bei 7 Patienten, die vor und unter der Imuran-behandlung untersucht wurden. Es wird vermutet, daß die Chromosomenabnormitäten bei den 19 mit Imuran behandelten Patienten wahrscheinlich nicht durch Imuran, sondern durch die Urämie entstanden sind.
Summary 19 patients treated with Imuran after a renal transplantation showed a high frequency of chromosome abnormalities, compared with 30 healthy control persons in the same age range. No significantly higher frequency of chromosome abnormalities was, however, found in 7 patients examined before and during treatment with Imuran. It is suggested that the chromosome abnormalities found in the 19 patients treated with Imuran were probably not due to Imuran but to Uremia.相似文献
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Schmidt H. Friedrich S. Danert K. Wuttky 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1959,29(8):375-376
Ohne Zusammenfassung 相似文献
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K. Friedrich D. Hüglin W. Seiffert H. W. Zimmermann 《Histochemistry and cell biology》1989,91(3):257-262
Summary Nuclei of Giemsa stained cells show a purple coloration, which is generated by a complex of DNA, azure B (AB) and eosin Y (EY). The structure of this complex is unknown. Its absorption spectrum shows a sharp and strong band at 18 100 cm–1 (552 nm), the so called Romanowsky band (RB). It is possible to produce the complex outside of the cell, but it is cubersome to handle. Easier to handle is a purple complex composed of chondroitin sulfate (CHS), AB and EY, which also shows a sharp and strong RB at 18100 cm–1 in the absorption spectrum. This CHS-AB-EY complex is a model for the DNA-AB-EY complex of Giemsa stained cell nuclei. We tried to investigate its structure.In the first step of the staining procedure CHS binds AB cations forming a stable CHS-AB complex. In the case of saturation each anionic SO
4
–
- and COO–-binding site of CHS is occupied by one dye cation and the complex has 1:1 composition. It has a strong and broad absorption band with its maximum at ca. 18000 cm–1 (556 nm). In the second step the CHS-AB complex additionally binds EY dianions forming the purple CHS-AB-EY complex with its RB at 18100 cm–1. This band can be clearly distinguished from the broad absorption of the bound AB cations. RB is generated by the EY chromophore, whose absorption is shifted to longer wavelength by the interaction with the CHS-AB framework.The CHS chains of the CHS-AB and CHS-AB-EY complexes can be mechanically aligned in a preferred direction k. Fine films of highly orientated complexes were prepared with a special technique and studied with a microspectrophotometer equipped with a polarizer and an analyzer. They are birefringent and dichroic-the more birefringent, the better the mechanical orientation. The sites of best orientation within the film were selected according to the quality of the birefringence. We measured the absorption of these regions with linearly polarized light. By setting the polarizer (e
p
parallel () or perpendicular () to k, we found that the transition moment m
AB
of the long wave-length absorption of AB in the CHS-AB and the CHS-AB-EY complexes is polarized almost perpendicular to the preferred direction k, m
AB
k. But the transition moment m
EY
of EY in CHS-AB-EY is polarized parallel to k, m
EY
k. The transition moments m
AB
and m
EY
lay in the molecular plane in the direction of the long axes of the AB and EY chromophores, respectively. Therefore, in both CHS-AB and CHS-AB-EY the long axes of the AB molecules are approximately perpendicular to the CHS chain; but in CHS-AB-EY the long axes of the EY chromophore are parallel to the chain of the biopolymer. This structure is somewhat surprising. In the CHS-AB-EY dye complex the chromophores of AB and EY are not parallel but approximately perpendicular to each other. 相似文献