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71.
James E. Olson Jeffrey T. Winston James A. Whitlock W. J. Pledger 《Journal of cellular physiology》1993,154(2):333-342
Density-arrested BALB/c-3T3 cells stimulated to proliferate in an amino acid-deficient medium arrest in mid-G1 at a point termed the V point. Cells released from V point arrest require 6 hr to traverse late G1 and enter S phase. As data presented here show that mRNA synthesis is needed for 2–3 hr after release of cells from the V point, after which inhibition of mRNA synthesis does not prevent entry into S phase, we used this mid-G1 arrest protocol to analyze gene expression in late G1. We found that although stimulation of cells in amino acid-deficient medium did not inhibit the induction of genes expressed in early G1, genes normally expressed in late G1 were expressed only after release from the V point. The expression of late G1 genes in cells released from the V point was temporally similar, in respect to G1 location, as was seen in stimulation of quiescent Go cells. As this protocol effectively divides gene expression into early (pre-V point) and late (post-V point) categories, it should be useful in studies of growth factor-modulated events that regulate traverse of late G1 and commitment to DNA synthesis. In addition, we used c-myb antisense oligonucleotides to show that c-myb expression, which occurs in late G1, is required for BALB/c-3T3 fibroblasts to traverse late G1 and initiate DNA synthesis. © 1993 Wiley-Liss, Inc. 相似文献
72.
Chemical evidence that chromatin DNA exists as 160 base pair beads interspersed with 40 base pair bridges. 下载免费PDF全文
Digestion of rat liver nuclei by an endogenous endonuclease generates double-stranded DNA fragments which are initially about 205 base pairs long, as reported previously by Hewish and Burgoyne. As digestion proceeds, the average size of these fragments is reduced to about 160 base pairs. Electrophoresis under denaturing conditions shows that these DNA fragments contain single strand nicks at ten base intervals. Fifteen bands, 10-150 bases, are clearly resolvable. DNA Fragments of 160 to 200 nucleotides are not resolved as distinct species. The results suggest that the chromosomal subunit contains both a 160 base-pair DNA segment, in a conformation susceptible to single strand nicking at ten base intervals, and a forty base-pair DNA segment in a conformation more uniformly susceptible to endogenous endonuclease activity. This chemical evidence agrees with morphological observations suggesting that chromatin has a "bead and bridge" structure. 相似文献
73.
Preparation and physical characterization of a homogeneous population of monomeric nucleosomes from HeLa cells. 总被引:15,自引:10,他引:5 下载免费PDF全文
We describe a method of isolating a homogeneous population of "trimmed" monomeric nucleosomes from Hela cells. These nucleoprotein particles contain a 140 +/- 5 base pair length of DNA and have a histone/DNA ratio of 1.2. They lack H1 and contain equal amounts of the four smaller histones. The DNA contains no single strand nicks. The particles sediment with an S20,w of 11S in D2O density gradients. After formaldehyde fixation, they band at a density of 1.4370 in neutral CsCl. Digestion of nucleosomes with either micrococcal nuclease or DNase I generates the same pattern of DNA fragments observed when intact nuclei are digested. Circular dichroism spectra indicate that the 280 nm positive ellipticity maximum of nucleosomes is about one-half that of chromatin. In the presence of 6 M urea, nucleosomes sediment with an S20,w of 6S, have a multiphasic thermal denaturation profile, and exhibit a circular dichroic spectrum nearly identical to that of B-form DNA. Our yield of purified nucleosomes (10-15% of the input DNA) is similar to the yields of other methods; our nucleosome population is substantially more homogeneous than those previously reported. 相似文献
74.
Lorena Luna-Mrquez Wyatt V Sharber Barbara A Whitlock Marcelo R Pace 《Annals of botany》2021,128(7):859
Background and Aims Byttneria is one of the few climbing genera in Malvaceae. Some Byttneria are known for their lobed stems. We explore the development of these stems, how they have evolved within the group and their relevance in the evolution of the climbing growth form in Malvaceae.MethodsWe combine developmental anatomical work with phylogenetic comparative methods. We use Byttneria divaricata and B. filipes as models in the anatomical work, a review of herbarium vouchers, and the most recent phylogeny of Byttneria and allies to elucidate how these stems evolved within the clade under maximum-likelihood and Bayesian approaches. We use Pagel94 tests to analyse the correlated evolution of lobed stems and prickles.Key ResultsEach lobe coincides with one of the five vascular bundles. By augmented activity of the fascicular cambium in the lobes coupled with reduced activity of the interfascicular cambium in the interlobes, secondary growth increases the lobulation already present during primary growth. Within Byttneria and allies, lobed young stems appeared at least three times, once in Ayenia and twice in the paraphyletic Byttneria. Lobed adult stems were conserved in Byttneria s.s., where lobed adult stems in combination with prickles were shown to have evolved as a climbing mechanism within the group; prickles were lost once within Byttneria s.s., in a shrubby subclade. Byttneria Clade 2 comprises climbers with twining cylindrical adult stems and no prickles, which constitutes a different climbing mechanism in the group.ConclusionsWe provide evidence of one of the few cambial variants known whose secondary body reflects the primary body vasculature and show that lobed adult stems and prickles in Byttneria could be used in the new delimitation of genera in the group. Lobed stems independently appeared in climbing Grewia, suggesting a convergence favouring the climbing growth form. 相似文献
75.
The incomplete natural history of mitochondria 总被引:35,自引:0,他引:35
Mitochondrial DNA (mtDNA) has been used to study molecular ecology and phylogeography for 25 years. Much important information has been gained in this way, but it is time to reflect on the biology of the mitochondrion itself and consider opportunities for evolutionary studies of the organelle itself and its ecology, biochemistry and physiology. This review has four sections. First, we review aspects of the natural history of mitochondria and their DNA to show that it is a unique molecule with specific characteristics that differ from nuclear DNA. We do not attempt to cover the plethora of differences between mitochondrial and nuclear DNA; rather we spotlight differences that can cause significant bias when inferring demographic properties of populations and/or the evolutionary history of species. We focus on recombination, effective population size and mutation rate. Second, we explore some of the difficulties in interpreting phylogeographical data from mtDNA data alone and suggest a broader use of multiple nuclear markers. We argue that mtDNA is not a sufficient marker for phylogeographical studies if the focus of the investigation is the species and not the organelle. We focus on the potential bias caused by introgression. Third, we show that it is not safe to assume a priori that mtDNA evolves as a strictly neutral marker because both direct and indirect selection influence mitochondria. We outline some of the statistical tests of neutrality that can, and should, be applied to mtDNA sequence data prior to making any global statements concerning the history of the organism. We conclude with a critical examination of the neglected biology of mitochondria and point out several surprising gaps in the state of our knowledge about this important organelle. Here we limelight mitochondrial ecology, sexually antagonistic selection, life-history evolution including ageing and disease, and the evolution of mitochondrial inheritance. 相似文献
76.
Ingvarsson PK Whitlock MC 《Proceedings. Biological sciences / The Royal Society》2000,267(1450):1321-1326
Individuals coming from the same subpopulation are more likely to share deleterious mutations at any given locus than hybrids formed between parents from different populations. Offspring of migrants therefore may experience heterosis and have higher fitness than resident individuals. This will, in turn, result in the immigrant alleles being present in higher frequencies than predicted from neutral expectations and thus a higher effective migration rate. In this paper we derive a formula to calculate the effective migration rate in the presence of heterosis. It is shown that the effect of heterosis on the migration rate can be substantial when fitness reduction within local populations is severe. The effect will be more pronounced in species with relatively short map lengths. Furthermore the heterosis effect will be highly variable throughout the genome, with the largest effect seen near selected genes and in regions of high gene density. 相似文献
77.
Jones LC Okino ST Gonda TJ Whitlock JP 《The Journal of biological chemistry》2002,277(25):22515-22519
We have studied the mechanism by which an acidic domain (amino acids 515-583) of the aromatic hydrocarbon receptor (AhR) transactivates a target gene. Studies with glutathione S-transferase fusion proteins demonstrate that the wild-type acidic domain associates in vitro with Myb-binding protein 1a, whereas a mutant domain (F542A, I569A) does not. AhR-defective cells reconstituted with an AhR containing the wild-type acidic domain exhibit normal AhR function; however, cells reconstituted with an AhR containing the mutant acidic domain do not function normally. Transient transfection of Myb-binding protein 1a into mouse hepatoma cells is associated with augmentation of AhR-dependent gene expression. Such augmentation does not occur when Myb-binding protein 1a is transfected into AhR-defective cells that have been reconstituted with an AhR that lacks the acidic domain. We infer that 1) Myb-binding protein 1a associates with AhR, thereby enhancing transactivation, and 2) the presence of AhR's acidic domain is both necessary and sufficient for Myb-binding protein 1a to increase AhR-dependent gene expression. 相似文献
78.
Variability in the time course of single photon responses from toad rods: termination of rhodopsin's activity. 总被引:4,自引:0,他引:4
We examined the responses of toad rod photoreceptors to single photons of light. To minimize the effects of variability in the early rising phase, we selected sets of responses that closely matched the rise of the mean single photon response. Responses selected in this way showed substantial variations in kinetics, appearing to peel off from a common time course after different delays. Following incorporation of the calcium buffer BAPTA, the time to peeling off was retarded. Our analysis indicates that it is not necessary to invoke a long series of reaction steps to explain the shutoff of rhodopsin activity. Instead, our results suggest that the observed behavior is explicable by the presently known shutoff reactions of activated rhodopsin, modulated by feedback. 相似文献
79.
Paul V. Fish Mark D. Andrews M. Jonathan Fray Alan Stobie Florian Wakenhut Gavin A. Whitlock 《Bioorganic & medicinal chemistry letters》2009,19(10):2829-2834
Single enantiomer [(aryloxy)(pyridinyl)methyl]piperidine and pyrrolidine derivatives 5–9 are inhibitors of monoamine reuptake. Structure–activity relationships established that monoamine reuptake inhibition are functions of amine, pyridine isomer, aryloxy ring substitution and stereochemistry. Consequently, selective NRIs, selective SRIs, dual SNRIs and triple SNDRIs were all identified. Dual SNRIs 5l–a and 9c were evaluated in additional pharmacology and pharmacokinetic studies as representative examples from this series. 相似文献
80.