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81.
Theresa L. Whiteside D. S. Heo S. Takagi J. T. Johnson S. Iwatsuki R. B. Herberman 《Cancer immunology, immunotherapy : CII》1988,26(1):1-10
Summary Lymphocytes infiltrating human solid tumors (TIL) and autologous peripheral blood lymphocytes (A-PBL) were cultured with 1000 units/ml of recombinant interleukin 2 (rIL2) in long-term cultures. TIL isolated from 26 primary squamous cell carcinomas of the head and neck expanded better (P<0.01) and achieved higher total lytic units of activity against fresh tumor cell targets (P<0.05) than A-PBL. TIL obtained from primary hepatocellular carcinomas (n=7) showed a higher degree of expansion than those from metastatic liver tumors (n=7). Further, TIL from metastatic tumors of the head and neck, liver, and ovary were delayed up to 50 days in their proliferative response to rIL2. Long-term mass cultures in rIL2 of TIL, A-PBL, or normal PBL were serially monitored for cytotoxicity with different cultured and fresh tumor cell targets and for phenotypic markers of the predominating cell populations. Antitumor cytotoxicity was found in cultures enriched in CD3 + Leu19 + and/or CD3-Leu19 + cells. Two-color sorting of such cultures followed by cytotoxicity assays confirmed that the human antitumor effectors expressed either the CD3 + Leu19 + or CD3-Leu19 + phenotype. CD3 + Leu19- cells had little or no antitumor cytotoxicity. The two types of Leu19 + effector cells were present in low numbers in fresh TIL, A-PBL, or normal PBL; in contrast, in some rIL2-expanded long-term cultures, they represented a majority of proliferating cells. This study identifies for the first time two types of antitumor effector cells in rIL2 cultures of human TIL, one of which may represent activated natural killer cells on the basis of the absence of the CD3 and expression of the Leu19 antigen. These antitumor effector cells mediate non-MHC-restricted cytotoxicity of fresh or cultured tumor cell targets of different histologic types. 相似文献
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WM. G. Moore 《The Western journal of medicine》1921,19(9):362-363
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Strauss L Bergmann C Szczepanski MJ Lang S Kirkwood JM Whiteside TL 《Journal of immunology (Baltimore, Md. : 1950)》2008,180(5):2967-2980
OBJECTIVE: Interaction of ICOS with its ligand (ICOSL, B7-H2) promotes T cell responses. As CD4+CD25highFoxp3+ naturally occurring T regulatory cells in melanoma patients express ICOS, we investigated the impact of ICOS on naturally occurring T regulatory cell function. METHODS: Expression of ICOS and T regulatory (Treg) cell markers was determined on CD4+CD25high T cells in PBMC and tumor-infiltrating lymphocytes from melanoma patients (n=10) and PBMC of normal controls (n=10) by multicolor flow cytometry. Suppression mediated by sorted ICOShigh and ICOSlow Treg was assessed in CFSE-based suppression assays with autologous CD4+CD25- responder cells (RC). Transwell inserts separating Treg from RC were used to evaluate suppression mechanisms used by Treg. ICOShigh or ICOSlow Treg were coincubated with RC+/-TCR and IL-2 stimulation. ICOShigh and ICOS- Treg were also expanded under conditions previously shown to induce Tr1 from RC. RESULTS: Treg in tumor-infiltrating lymphocytes expressed ICOS (mean fluorescence intensity=70+/-10), while Treg in PBMC had low ICOS expression (mean fluorescence intensity=3.5+/-2.5, p相似文献
90.
André M Hidalgo John WM Bastiaansen Barbara Harlizius Hendrik-Jan Megens Ole Madsen Richard PMA Crooijmans Martien AM Groenen 《BMC genetics》2014,15(1):1-13