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51.
The majority of bacteria engaged in bioluminescent symbiosis are environmentally acquired and facultatively symbiotic. A few enigmatic bioluminescent symbionts have not been successfully cultured, which has led to speculation that they may be obligately dependent on their hosts. Here, we report the draft genome of the uncultured luminous symbiont of an anomalopid flashlight fish, ‘Candidatus Photodesmus katoptron’. The genome of the anomalopid symbiont is reduced by 80% compared with close relatives and lacks almost all genes necessary for amino acid synthesis and for metabolism of energy sources other than glucose, supporting obligate dependence on the host for growth. ‘Candidatus Photodesmus katoptron’ is the first described obligate mutualistic symbiont of a vertebrate. Unlike most other obligate mutualists, the anomalopid symbiont genome has retained complete pathways for chemotaxis and motility as well as most genes involved in cell wall production, consistent with the hypothesis that these bacteria may be transmitted environmentally during an extra‐host phase. 相似文献
52.
Miller JL de Wet BJ deWet BJ Martinez-Pomares L Radcliffe CM Dwek RA Rudd PM Gordon S 《PLoS pathogens》2008,4(2):e17
Macrophages (MØ) and mononuclear phagocytes are major targets of infection by dengue virus (DV), a mosquito-borne flavivirus that can cause haemorrhagic fever in humans. To our knowledge, we show for the first time that the MØ mannose receptor (MR) binds to all four serotypes of DV and specifically to the envelope glycoprotein. Glycan analysis, ELISA, and blot overlay assays demonstrate that MR binds via its carbohydrate recognition domains to mosquito and human cell–produced DV antigen. This binding is abrogated by deglycosylation of the DV envelope glycoprotein. Surface expression of recombinant MR on NIH3T3 cells confers DV binding. Furthermore, DV infection of primary human MØ can be blocked by anti-MR antibodies. MR is a prototypic marker of alternatively activated MØ, and pre-treatment of human monocytes or MØ with type 2 cytokines (IL-4 or IL-13) enhances their susceptibility to productive DV infection. Our findings indicate a new functional role for the MR in DV infection. 相似文献
53.
Noël Cameron John Pettifor Thea De Wet Shane Norris 《Obesity (Silver Spring, Md.)》2003,11(3):457-460
Objective: Children with birth weight appropriate for gestational age (AGA) who also demonstrate rapid weight gain in infancy have a greater risk of being overweight or obese during childhood. A concurrent advancement in skeletal maturity would account for their greater size and would, therefore, not necessarily pose a threat of greater risk during adolescence and early adulthood. This study aims to determine whether children with rapid weight gain during infancy have advanced skeletal maturity during childhood. Research Methods and Procedures: One hundred and ninety‐three African children (boys = 108; girls = 85) of normal birth weight and gestational age were assessed from birth to 9 years. Body composition was assessed at 9 years of age by whole‐body DXA, and skeletal maturity was assessed using the Tanner‐Whitehouse II technique. Rapid weight gain in infancy was defined as a +0.67 change in weight‐for‐age Z‐score between birth and 2 years. Results: Rapid weight gain was experienced by over 20% of the sample. Children with rapid weight gain were significantly lighter at birth and significantly taller, heavier, and fatter throughout childhood. Chronological age and Tanner‐Whitehouse II technique skeletal ages at 9 years were not significantly different between groups or between sexes within groups. Discussion: Because AGA children with rapid weight gain have a greater risk of overweight and obesity but are not advanced in skeletal maturity, later adolescent adjustments toward average weight and fatness values are unlikely. The identification and monitoring of such children is of importance in reducing their risk of morbidity. 相似文献
54.
J R de Wet D L Daniels J L Schroeder B G Williams K Denniston-Thompson D D Moore F R Blattner 《Journal of virology》1980,33(1):401-410
The mapping of the sites of cleavage of nine restriction endonucleases (EcoRI, HindIII, BamHI, SalI, KpnI, SstI, BglII, XhoI, and XbaI) on 21 Charon phage vectors is described. Maps of individual subsections were obtained and then combined to assemble the complete vector maps. Calculations of maximum and minimum sizes of inserts which may be carried by the vectors using different restriction endonucleases or pairs of restriction endonucleases are presented. The regions mapped include several parts of phi 80 that had not been mapped previously. 相似文献
55.
56.
Derivatives of a cross between diploid Zea mays L. and Tripsacum dactyloides (L.) L. (2n = 72) were compared cytologically and morphologically. The objective of this study was to detect introgression from Tripsacum to maize that might have occurred during seven backcross generations with maize. Thirty-three morphological characters were used to analyze variation among aneuploid (20Zm + 2Td), 20-chromosome recovered maize, and the recurrent maize parent plants. Aneuploid and maize checks were extreme types, with 20-chromosome hybrid derivatives being morphologically intermediate. Several recovered maizes clustered with aneuploid plants and these hybrid derivatives have the greatest chance of Tripsacum introgression. Many traits such as endosperm abnormalities, tassel seed, albinos, tunicate glumes, tassel-tipped ears, fasciated and branched ear, and male spikelets between rows of kernels were observed. Although the genetic basis of many traits is unknown, mutations, epistatic effects or expression of Tripsacum chromatin are possible causes. The number of abnormal and tripsacoid traits observed in 20-chromosome recovered maizes indicates genetic transfer from Tripsacum to the maize genome. 相似文献
57.
The nature of the complexes and equilibria shown by solutions of protohaemin in dimethyl sulphoxide/water mixtures and in the presence of acid and base were studied by u.v.-visible spectrophotometry. In neutral solutions containing from 40 to 100% dimethyl sulphoxide, haemin is present as a monomeric complex in which the Cl-ion is not coordinated. Only a single pH-dependent equilibrium pK12 is observed over the range 40-80% dimethylsulphoxide, corresponding to formation of the mu-oxo dimer. As the dimethyl sulphoxide content is lowered below 35%, so the single equilibrium (pK12) is replaced by two equilibria (pK1 and pK2); with solutions of 5 microM-haemin, pK1 decreases (from pK12 7.55 in 65% dimethyl sulphoxide to pK1 approx. 1.5 in 0.01% dimethyl sulphoxide), whereas pK2 hardly changes (from pK12 7.55 in 65% to pK2 approx. 7.5 in 0.01%). 相似文献
58.
J. M. J. de Wet K. E. Prasada Rao D. E. Brink M. H. Mengesha 《American journal of botany》1984,71(4):550-557
Finger millet (Eleusine coracana (L.) Gaertn. subsp. coracana) is cultivated in eastern and southern Africa and in southern Asia. The closest wild relative of finger millet is E. coracana subsp. africana (Kennedy-O'Byrne) Hilu & de Wet. Wild finger millet (subsp. africana) is native to Africa but was introduced as a weed to the warmer parts of Asia and America. Derivatives of hybrids between subsp. coracana and subsp. africana are companion weeds of the crop in Africa. Cultivated finger millets are divided into five races on the basis of inflorescence morphology. Race coracana is widely distributed across the range of finger millet cultivation. It is present in the archaeological record of early African agriculture that may date back 5,000 years. Racial evolution took place in Africa. Races vulgaris, elongata, plana, and compacta evolved from race coracana, and were introduced into India some 3,000 years ago. Little independent racial evolution took place in India. 相似文献
59.
The widely distributed and morphologically variable grass species Bothriochloa intermedia (R. Br.) A. Camus was studied in detail. Morphological and cytogenetical observations indicated that variation is due to interspecific and intergeneric hybridization. The species B. grahamii (Haines) Bor and B. glabra (Roxb.) A. Camus represent intergeneric hybrid derivatives between B. intermedia and Dichanthium annulatum (Forssk.) Stapf, and between B. intermedia and Capillipedium parviflorum (R. Br.) Stapf, respectively. These intergeneric hybrids, and also the apomictic hybrid derivatives of both B. grahamii and B. intermedia with the related B. ischaemum (L.) Keng, are included in the compilospecies. Morphological and cytogenetical studies suggested that the apomictic B. odorata (Lisboa) A. Camus and B. haenkei (Presl) Ohwi are biotypes of B. glabra. The genera involved, Bothriochloa O. Kuntze, Capillipedium Stapf and Dichanthium Willemet, behave phylogenetically like sections of a single genus, with the last-mentioned generic name taking priority. 相似文献
60.
J. M. J. de Wet 《American journal of botany》1967,54(3):384-387
Essential oil components and gross morphological characters are closely correlated in Dichanthium parviflorum (R. Br.) de Wet et Harlan (Gramineae) and related species. Different species, varieties, and geographical races, as well as hybrids between them, can be identified on the basis of absence or presence and quantity of essential oil components. The morphologically variable D. parviflorum was subdivided into four varieties: var. parviflorum, var. capilliflorum (Steud.) de Wet et Harlan comb. nov., var. mutispiculum (Ohwi) de Wet et Harlan comb. nov., and var. spicigerum (S. T. Blake) de Wet et Harlan comb. nov. These varieties differ from each other morphologically in having respectively racemes with 1-4 and awned, 3-5 and awned, 1-2 and awnlass, and 4-10 and awned spikelet pairs per raceme. 相似文献