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971.
972.
Mrówka P Głodkowska E Młynarczuk-Biały I Biały L Kuckelkorn U Nowis D Makowski M Legat M Gołab J 《Acta biochimica Polonica》2008,55(1):75-84
Thiazolidinediones are oral antidiabetic agents that activate peroxisome proliferator-activated receptor-gamma (PPAR-gamma) and exert potent antioxidant and anti-inflammatory properties. It has also been shown that PPAR-gamma agonists induce G0/G1 arrest and apoptosis of malignant cells. Some of these effects have been suggested to result from inhibition of proteasome activity in target cells. The aim of our studies was to critically evaluate the cytostatic/cytotoxic effects of one of thiazolidinediones (pioglitazone) and its influence on proteasome activity. Pioglitazone exerted dose-dependent cytostatic/cytotoxic effects in MIA PaCa-2 cells. Incubation of tumor cells with pioglitazone resulted in increased levels of p53 and p27 and decreased levels of cyclin D1. Accumulation of polyubiquitinated proteins within cells incubated with pioglitazone suggested dysfunction of proteasome activity. However, we did not observe any influence of pioglitazone on the activity of isolated proteasome and on the proteolytic activity in lysates of pioglitazone-treated MIA PaCa-2 cells. Further, treatment with pioglitazone did not cause an accumulation of fluorescent proteasome substrates in transfected HeLa cells expressing unstable GFP variants. Our results indicate that pioglitazone does not act as a direct or indirect proteasome inhibitor. 相似文献
973.
Tomasz Podgórski Krzysztof Schmidt Rafał Kowalczyk Agnieszka Gulczyńska 《Acta theriologica》2008,53(2):97-110
We studied microhabitat selection of the Eurasian lynxLynx lynx (Linnaeus, 1758) at 116 hunting and 88 resting sites in Białowieża Primeval Forest (Poland) to describe its characteristics
and determine the importance of habitat structure for stalking prey and for security during resting. We identified lynx-used
sites by radio-tracking 3 male and 3 female lynx. When hunting, the lynx did not select for any type or age class of forest.
During both summer and winter, the lynx selected sites characterized by high complexity (number of structures useful for stalking:
fallen logs and branches, root plates, patches of dense bushes) and low visibility. In summer, hunting sites were often located
in the vicinity of small forest glades that provided good stalking opportunities for lynx and rich foraging resources for
roe deer — the main prey of lynx. The habitat at kill sites was more open than at sites where the prey was cached, with higher
visibility, lower density of trees and poorer undergrowth. The most important characteristic of resting sites was very low
visibility that resulted mainly from using young pine or spruce thickets in the winter and dense undergrowth of oak-lime-hornbeam
and ash-alder forests in the summer. The information provided by this study could have direct implications for Eurasian lynx
conservation by guiding forest restructuring to better suit the species’ biological requirements. 相似文献
974.
Wysocka M Lesner A Guzow K Mackiewicz L Legowska A Wiczk W Rolka K 《Analytical biochemistry》2008,378(2):208-215
In this study, chemical synthesis of the selective chromogenic/fluorogenic substrates for proteinase 3 is described. The substrates’ sequence was obtained using combinatorial chemistry methods. Deconvolution of the tripeptide library against proteinase 3 with general formula ABZ-X3-X2-X1-ANB-NH2 yielded the active sequence. Selected peptide was further modified on its C terminus to investigate the impact of chromophore moiety modification on enzyme-substrate interaction. To determine specificity, activity of selected substrates was characterized against proteinase 3 and neutrophil elastase. Finally, the peptide ABZ-Tyr-Tyr-Abu-ANB-NH2 displayed the highest value of specificity constant (kcat/KM = 189 × 103 M−1 s−1) for proteinase 3. To the best of our knowledge, this is the first short peptide that undergoes selective proteolysis by proteinase 3 and displays no significant hydrolysis in the presence of human neutrophil elastase and cathepsin G. 相似文献
975.
Phytosociological and habitat studies of the phytocoenoses of Luronium natans have been conducted. The present results were compared with data on L. dortmanna and I. lacustris. It is demonstrated that the community of L. natans differs from the other two communities with respect to habitat conditions despite the fact that they have been reported to occur jointly and alongside in Lobelia lakes. It appears that significant differences between the communities are found not only regarding their waters, but also their substrates. L. natans dominated phytocoenoses are confined to oligotrophic, extremely soft waters, markedly poor in Ca2+, but richer in Na+ and SO4
2- than those of Lobelia and Isoëtes. Luronium natans develops best on acidic, highly hydrated substrates rich in organic matter, NO3
– and total N. The results obtained indicate that L. natans and the phytocoenoses formed by it are characterized by their narrow ecological amplitude in Poland as opposed to those occurring in western Europe, which tolerate a relatively wide range of habitats. The present findings confirm the data from numerous works, which point to the weak competitive ability of the species compared with species typical of eutrophic waters. 相似文献
976.
Paweł Mackiewicz Dorota Mackiewicz Agnieszka Gierlik Maria Kowalczuk Aleksandra Nowicka Małgorzata Dudkiewicz Mirosław R. Dudek Stanisław Cebrat 《Journal of molecular evolution》2001,53(6):615-621
We have elaborated a method which has allowed us to estimate the direction of translocation of orthologs which have changed,
during the phylogeny, their positions on chromosome in respect to the leading or lagging role of DNA strands. We have shown
that the relative number of translocations which have switched positions of genes from the leading to the lagging DNA strand
is lower than the number of translocations which have transferred genes from the lagging strand to the leading strand of prokaryotic
genomes. This paradox could be explained by assuming that the stronger mutation pressure and selection after inversion preferentially
eliminate genes transferred from the leading to the lagging DNA strand.
Received: 12 December 2000 / Accepted: 20 April 2001 相似文献
977.
A. Jurkiewicz K. Turnau J. Mesjasz-Przybyłowicz W. Przybyłowicz B. Godzik 《Protoplasma》2001,218(3-4):117-124
Summary The metal distribution within mycorrhizal and nonmycorrhizal roots ofEpipactis atrorubens collected from zinc mine tailings and an area rich in heavy metal ores (both located in southern Poland) was investigated. The tailings, consisting of postflotation material, were characterised by high levels of toxic elements such as Zn, Pb, and Cd, while soil outside the tailings was also strongly enriched in heavy metals. Atomic absorption spectrometry and proton-induced X-ray emission analysis revealed that heavy metals were mostly accumulated within orchid roots. Elemental maps from proton-induced X-ray emission showed that plant root epidermis and fungal coils which had developed within cortical cells of roots collected from the zinc mine tailings were the main places of Zn and Pb accumulation, associated with increased concentrations of Fe, Cd, Ti, Mn, Si, Ca, and S. The mean content of Pb and Zn in the coils was 4 to 5 times higher than in the root epidermis. In mycorrhizal roots from the tailings a statistically significant decrease in Pb and Zn content towards the inside of the root was observed. The mean content of Pb in coils from roots of plants growing outside the tailings was about 1% of the concentration in root coils from the tailings. Coils selected from orchid roots originating from a site outside the tailings contained comparatively high concentrations of Zn, Cd, and Cu, which was probably due to the high content of these elements in the soil. The results presented suggest a biofiltering effect against heavy metals by orchid mycorrhizal fungi. 相似文献
978.
Large-scale preparation of highly purified tonoplast from cucumber (Cucumis sativus L.) roots was obtained after centrifugation of microsome pellet (10,000 – 80,000 g) on discontinuous sucrose density gradient
(20, 28, 32 and 42 %). Lack of PEP carboxylase (cytosol marker) and cytochrome c oxidase (mitochondrial marker) together with
a slight activity of VO4-ATPase (plasma membrane marker) and NADH-cytochrome c reductase (ER marker) in tonoplast preparation confirmed its high purity.
Using latency of nitrate-inhibited ATPase and H+ pumping as criteria it was established that the majority of tonoplast vesicles were sealed and oriented right(cytoplasmic)-side-out.
Strong acidification of the interior of vesicles observed at the presence of both, ATP and PPi, confirmed that obtained tonoplast contains two classes of proton pumps: V-ATPase and H+PPiase.
To examine and characterise of proton-transport systems in tonoplast, the effect of various inhibitors on H+ pumping and hydrolytic activities of ATPase and PPiase were measured. ATP-dependent activities (H+ flux and ATP hydrolysis) were specifically decreased by nitrate and bafilomycin A1, whereas the PPiase activities were reduced in the presence of fluoride and Na+ ions. Both enzymes showed a similar sensitivity to DCCD and DES. The results of experiments with KCl and NaCl suggested that
the vacuolar ATPase was stimulated by Cl−, whereas the vacuolar Ppiase requires K+ ions for its activity. 相似文献
979.
Joanna Augustynowicz Małgorzata Lekka Kveta Burda Halina Gabryś 《Acta Physiologiae Plantarum》2001,23(3):291-302
Translocations of chloroplasts induced by blue light were investigated in both leaves and protoplasts isolated from leaf mesophyll
of Nicotiana tabacum. In the leaf tissue, the responses of chloroplasts were similar to those observed in other, higher and lower plant species.
Weak and strong light induced movements of chloroplasts towards cell walls perpendicular and parallel to the light direction,
respectively. Treatment with cytochalasin D, an actin-disturbing agent, blocked the movements. This shows that actin is involved
in the motile system of chloroplast translocation in tobacco. By monitoring the response of chloroplasts to light in isolated
protoplasts, we addressed the question whether the presence of the cell wall is necessary for the translocations of chloroplasts
to occur. In control protoplasts (isolated at room temperature from unstressed leaves), no clear light intensity-dependent
changes were observed in chloroplast distribution pattern. In contrast, in protoplasts obtained from plants treated with 4
°C for 8 h the chloroplasts maintained their responsiveness to light. Atomic Force Microscopy was used to measure elastic
properties of the protoplasts. Young’s modulus, which reflects rigidity of the material, was 10 times higher for protoplasts
of the coldstressed plants as compared to those isolated from the control plants. The rigidity of protoplasts isolated from
the plants treated with low temperature was reduced four-fold by exposure to cytochalasin D. It appears that the status of
protoplast actin is a factor responsible for elasticity of protoplasts. We speculate that unknown, cold stress-induced factors,
maintain the orientational movements due to anchorage of the actin cytoskeleton in the plasma membrane despite the cell wall
removal. 相似文献
980.
Stanisław Weidner Ewa Frączek Ryszard Amarowicz Shunnosuke Abe 《Acta Physiologiae Plantarum》2001,23(4):475-482
A series of high pressure liquid chroamtography analyses revealed the presence of five phenolic acids in rye caryopses (vanillic,
caffeic, p-coumaric, ferulic and sinapic), three of which (p-coumaric, ferulic and sinapic) were found in the free phenolic
fraction. Ferulic acid was predominant, both among free acids and total phenolic acids (i.e. free, liberated from soluble
esters and glycosides). The highest content of the free phenolic acids in rye caryopses was observed at the beginning of development,
when on 22 DAF it was estimated at 11.55 μg·g−1 DW. During dehydratation the total level of free phenolic acids in rye caryopses decreased in all investigated samples. Although
total phenolic acids contents in all samples of unripe rye caryopses always decreased after dehydration, in rye sample collected
in full ripeness (57 DAF), the amount of these compounds increased after the enforced dehydration. It should be added that
in ester-bound-soluble phenolic acids fraction (the largest part in the total phenolic acids fraction), irrespective of the
total amount decrease, much increase of sinapic acid content in this fraction was observed after dehydratation treatment in
all investigated samples of caryopses of various ripeness. During the development and ripening of rye caryopses, a gradual
increase in the precocious germination ability of the grain was observed. The enforced dehydration stimulated the process
of precocious germination of developing and ripening rye caryopses. A possible role of phenolics in preventing precocious
germination and acclimation to dehydration of developing and ripening rye grains is discussed. 相似文献