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Carla Rossini Crepaldi Phelipe Augusto Mariano Vitale Andrea Cristina Tesch Hélen Julie Laure José César Rosa Marcelo de Cerqueira César 《Mitochondrion》2013,13(6):823-830
Two types of binding sites for hexokinase, designated as Type A or Type B sites, have been shown to coexist on brain mitochondria. The ratio of these sites varies between species.HK1 attaches by reversibly binding to the voltage dependent anion channel (VDAC). Regarding the nature of hexokinase binding sites, we investigated if it was linked to distinct VDAC interactomes. We approached this question by 2D BN/SDS-PAGE of mitochondria, followed by mass spectrometry.Our results are consistent with the possibility that the ratio of Type A/Type B sites is due to differential VDAC interactions in bovine and rat neuronal cells. 相似文献
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Augusto García Villanueva María Jesús García Villanueva Mercedes García Villanueva Roberto Rojo Blanco María Vicenta Collado Guirao Jacobo Cabañas Montero Rafael Beni Pérez Irene Moreno Montes 《Endocrinología y nutrición》2013,60(5):254-259
Amyloidosis is an uncommon syndrome consisting of a number of disorders having in common an extracellular deposit of fibrillary proteins. This results in functional and structural changes in the affected organs, depending on deposit location and severity.Amyloid infiltration of the thyroid gland may occur in 50% and up to 80% of patients with primary and secondary amyloidosis respectively. Amyloid goiter (AG) is a true rarity, usually found associated to secondary amyloidosis. AG may require surgical excision, usually because of compressive symptoms.We report the case of a patient with a big AG occurring in the course of a secondary amyloidosis associated to polyarticular onset juvenile idiopathic arthritis who underwent total thyroidectomy. Current literature is reviewed, an attempt is made to provide action guidelines, and some surgical considerations on this rare condition are given. 相似文献
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Anne Drumond Villela Rodrigo Gay Ducati Leonardo Astolfi Rosado Carlos Junior Bloch Maura Vianna Prates Danieli Cristina Gon?alves Carlos Henrique Inacio Ramos Luiz Augusto Basso Diogenes Santiago Santos 《PloS one》2013,8(2)
Uracil phosphoribosyltransferase (UPRT) catalyzes the conversion of uracil and 5-phosphoribosyl-α-1-pyrophosphate (PRPP) to uridine 5′-monophosphate (UMP) and pyrophosphate (PPi). UPRT plays an important role in the pyrimidine salvage pathway since UMP is a common precursor of all pyrimidine nucleotides. Here we describe cloning, expression and purification to homogeneity of upp-encoded UPRT from Mycobacterium tuberculosis (MtUPRT). Mass spectrometry and N-terminal amino acid sequencing unambiguously identified the homogeneous protein as MtUPRT. Analytical ultracentrifugation showed that native MtUPRT follows a monomer-tetramer association model. MtUPRT is specific for uracil. GTP is not a modulator of MtUPRT ativity. MtUPRT was not significantly activated or inhibited by ATP, UTP, and CTP. Initial velocity and isothermal titration calorimetry studies suggest that catalysis follows a sequential ordered mechanism, in which PRPP binding is followed by uracil, and PPi product is released first followed by UMP. The pH-rate profiles indicated that groups with pK values of 5.7 and 8.1 are important for catalysis, and a group with a pK value of 9.5 is involved in PRPP binding. The results here described provide a solid foundation on which to base upp gene knockout aiming at the development of strategies to prevent tuberculosis. 相似文献
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Ferlosio Amedeo Doldo Elena Agostinelli Sara Costanza Gaetana Centofanti Federica Sidoni Angelo Orlandi Augusto 《Molecular biology reports》2020,47(9):6879-6886
Molecular Biology Reports - In recent years, new treatments with novel action mechanisms have been explored for advanced non-small cell lung cancer (NSCLC). Retinoids promote cancer cell... 相似文献
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Anton Débora Bublitz Guzman Frank Lino Vetö Nicole Moreira Krause Felipe Augusto Kulcheski Franceli Rodrigues Coelho Ana Paula Durand Duarte Guilherme Leitão Margis Rogério Dillenburg Lúcia Rebello Turchetto-Zolet Andreia Carina 《Molecular biology reports》2020,47(2):1033-1043
Molecular Biology Reports - Eugenia uniflora is an Atlantic Forest native species, occurring in contrasting edaphoclimatic environments. The identification of genes involved in response to abiotic... 相似文献
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Mateusz Siedlinski Dustin Tingley Peter J. Lipman Michael H. Cho Augusto A. Litonjua David Sparrow Per Bakke Amund Gulsvik David A. Lomas Wayne Anderson Xiangyang Kong Stephen I. Rennard Terri H. Beaty John E. Hokanson James D. Crapo Christoph Lange Edwin K. Silverman 《Human genetics》2013,132(4):431-441
Cigarette smoking is the major environmental risk factor for chronic obstructive pulmonary disease (COPD). Genome-wide association studies have provided compelling associations for three loci with COPD. In this study, we aimed to estimate direct, i.e., independent from smoking, and indirect effects of those loci on COPD development using mediation analysis. We included a total of 3,424 COPD cases and 1,872 unaffected controls with data on two smoking-related phenotypes: lifetime average smoking intensity and cumulative exposure to tobacco smoke (pack years). Our analysis revealed that effects of two linked variants (rs1051730 and rs8034191) in the AGPHD1/CHRNA3 cluster on COPD development are significantly, yet not entirely, mediated by the smoking-related phenotypes. Approximately 30 % of the total effect of variants in the AGPHD1/CHRNA3 cluster on COPD development was mediated by pack years. Simultaneous analysis of modestly (r 2 = 0.21) linked markers in CHRNA3 and IREB2 revealed that an even larger (~42 %) proportion of the total effect of the CHRNA3 locus on COPD was mediated by pack years after adjustment for an IREB2 single nucleotide polymorphism. This study confirms the existence of direct effects of the AGPHD1/CHRNA3, IREB2, FAM13A and HHIP loci on COPD development. While the association of the AGPHD1/CHRNA3 locus with COPD is significantly mediated by smoking-related phenotypes, IREB2 appears to affect COPD independently of smoking. 相似文献