全文获取类型
收费全文 | 2943篇 |
免费 | 209篇 |
国内免费 | 1篇 |
出版年
2023年 | 27篇 |
2022年 | 31篇 |
2021年 | 91篇 |
2020年 | 71篇 |
2019年 | 73篇 |
2018年 | 84篇 |
2017年 | 86篇 |
2016年 | 98篇 |
2015年 | 172篇 |
2014年 | 153篇 |
2013年 | 219篇 |
2012年 | 204篇 |
2011年 | 215篇 |
2010年 | 141篇 |
2009年 | 102篇 |
2008年 | 161篇 |
2007年 | 155篇 |
2006年 | 143篇 |
2005年 | 132篇 |
2004年 | 122篇 |
2003年 | 100篇 |
2002年 | 95篇 |
2001年 | 20篇 |
2000年 | 21篇 |
1999年 | 26篇 |
1998年 | 18篇 |
1997年 | 15篇 |
1996年 | 21篇 |
1995年 | 15篇 |
1994年 | 18篇 |
1993年 | 15篇 |
1992年 | 15篇 |
1991年 | 18篇 |
1990年 | 14篇 |
1989年 | 14篇 |
1988年 | 7篇 |
1987年 | 6篇 |
1986年 | 6篇 |
1985年 | 15篇 |
1984年 | 15篇 |
1983年 | 14篇 |
1982年 | 11篇 |
1981年 | 6篇 |
1980年 | 10篇 |
1979年 | 9篇 |
1977年 | 7篇 |
1976年 | 8篇 |
1974年 | 8篇 |
1973年 | 9篇 |
1970年 | 6篇 |
排序方式: 共有3153条查询结果,搜索用时 31 毫秒
61.
Peter T. Euclide Garrett J. McKinney Matthew Bootsma Charlene Tarsa Mariah H. Meek Wesley A. Larson 《Molecular ecology resources》2020,20(1):66-78
Interpretation of high‐throughput sequence data requires an understanding of how decisions made during bioinformatic data processing can influence results. One source of bias that is often cited is PCR clones (or PCR duplicates). PCR clones are common in restriction site‐associated sequencing (RAD‐seq) data sets, which are increasingly being used for molecular ecology. To determine the influence PCR clones and the bioinformatic handling of clones have on genotyping, we evaluate four RAD‐seq data sets. Data sets were compared before and after clones were removed to estimate the number of clones present in RAD‐seq data, quantify how often the presence of clones in a data set causes genotype calls to change compared to when clones were removed, investigate the mechanisms that lead to genotype call changes and test whether clones bias heterozygosity estimates. Our RAD‐seq data sets contained 30%–60% PCR clones, but 95% of RAD‐tags had five or fewer clones. Relatively few genotypes changed once clones were removed (5%–10%), and the vast majority of these changes (98%) were associated with genotypes switching from a called to no‐call state or vice versa. PCR clones had a larger influence on genotype calls in individuals with low read depth but appeared to influence genotype calls at all loci similarly. Removal of PCR clones reduced the number of called genotypes by 2% but had almost no influence on estimates of heterozygosity. As such, while steps should be taken to limit PCR clones during library preparation, PCR clones are likely not a substantial source of bias for most RAD‐seq studies. 相似文献
62.
Walter Rossi Cervi Rubens Augusto Camargo Lamparelli Joaquim Eugênio Abel Seabra Martin Junginger Sierk de Jong Floor van der Hilst 《Global Change Biology Bioenergy》2020,12(2):136-157
It is expected that Brazil could play an important role in biojet fuel (BJF) production in the future due to the long experience in biofuel production and the good agro‐ecological conditions. However, it is difficult to quantify the techno‐economic potential of BJF because of the high spatiotemporal variability of available land, biomass yield, and infrastructure as well as the technological developments in BJF production pathways. The objective of this research is to assess the recent and future techno‐economic potential of BJF production in Brazil and to identify location‐specific optimal combinations of biomass crops and technological conversion pathways. In total, 13 production routes (supply chains) are assessed through the combination of various biomass crops and BJF technologies. We consider temporal land use data to identify potential land availability for biomass production. With the spatial distribution of the land availability and potential yield of biomass crops, biomass production potential and costs are calculated. The BJF production cost is calculated by taking into account the development in the technological pathways and in plant scales. We estimate the techno‐economic potential by determining the minimum BJF total costs and comparing this with the range of fossil jet fuel prices. The techno‐economic potential of BJF production ranges from 0 to 6.4 EJ in 2015 and between 1.2 and 7.8 EJ in 2030, depending on the reference fossil jet fuel price, which varies from 19 to 65 US$/GJ across the airports. The techno‐economic potential consists of a diverse set of production routes. The Northeast and Southeast region of Brazil present the highest potentials with several viable production routes, whereas the remaining regions only have a few promising production routes. The maximum techno‐economic potential of BJF in Brazil could meet almost half of the projected global jet fuel demand toward 2030. 相似文献
63.
Livia Pilatti PhD Renato Mancini Astray Mayra Pereira Rocca Flavia Ferreira Barbosa Soraia Attie Calil Jorge Michael Butler Elisabeth de Fátima Pires Augusto 《Biotechnology progress》2020,36(6):e3046
Most rabies vaccines are based on inactivated virus, which production process demands a high level of biosafety structures. In the past decades, recombinant rabies virus glycoprotein (RVGP) produced in several expression systems has been extensively studied to be used as an alternative vaccine. The immunogenic characteristics of this protein depend on its correct conformation, which is present only after the correct post-translational modifications, typically performed by animal cells. The main challenge of using this protein as a vaccine candidate is to keep its trimeric conformation after the purification process. We describe here a new immunoaffinity chromatography method using a monoclonal antibody for RVGP Site II for purification of recombinant rabies virus glycoprotein expressed on the membrane of Drosophila melanogaster S2 cells. RVGP recovery achieved at least 93%, and characterization analysis showed that the main antigenic proprieties were preserved after purification. 相似文献
64.
dos Santos Fernanda Marcelia Pflüger Pricila Fernandes Lazzarotto Leticia Uczay Mariana de Aguida Wesley Roberto da Silva Lisiane Santos Boaretto Fernanda Brião Menezes de Sousa Jayne Torres Picada Jaqueline Nascimento da Silva Torres Iraci Lucena Pereira Patrícia 《Neurochemical research》2021,46(8):2066-2078
Neurochemical Research - Gamma-decanolactone (GD) has been shown to reduce epileptic behavior in different models, inflammatory decreasing, oxidative stress, and genotoxic parameters. This study... 相似文献
65.
Rodrigues Augusto Pereira Fabrício F. Barbosa Paulo R.R. Silva-Torres Christian S.A. Torres Jorge B. 《Journal of Insect Behavior》2021,34(3):71-81
Journal of Insect Behavior - Control of the sugarcane borers, Diatraea saccharalis and Diatraea impersonatella (= D. flavipennella) (Lepidoptera: Crambidae), in Brazil, is based on mass release of... 相似文献
66.
Kuczenski Brandon Mutel Chris Srocka Michael Scanlon Kelly Ingwersen Wesley 《The International Journal of Life Cycle Assessment》2021,26(3):483-496
The International Journal of Life Cycle Assessment - The flexibility of life cycle inventory (LCI) background data selection is increasing with the increasing availability of data, but this comes... 相似文献
67.
68.
Manfred Schartl Susanne Kneitz Jenny Ormanns Cornelia Schmidt Jennifer L. Anderson Angel Amores Julian Catchen Catherine Wilson Dietmar Geiger Kang Du Mateo Garcia-Olazábal Sudha Sudaram Christoph Winkler Rainer Hedrich Wesley C. Warren Ronald Walter Axel Meyer John H. Postlethwait 《Current biology : CB》2021,31(5):911-922.e4
69.
Olivier Maurin Artemis Anest Sidonie Bellot Edward Biffin Grace Brewer Tristan Charles-Dominique Robyn S. Cowan Steven Dodsworth Niroshini Epitawalage Berta Gallego Augusto Giaretta Renato Goldenberg Deise J.P. Gonçalves Shirley Graham Peter Hoch Fiorella Mazine Yee Wen Low Catherine McGinnie Fabián A. Michelangeli Sarah Morris Darin S. Penneys Oscar Alejandro Pérez Escobar Yohan Pillon Lisa Pokorny Gustavo Shimizu Vanessa G. Staggemeier Andrew H. Thornhill Kyle W. Tomlinson Ian M. Turner Thais Vasconcelos Peter G. Wilson Alexandre R. Zuntini William J. Baker Félix Forest Eve Lucas 《American journal of botany》2021,108(7):1087-1111
70.