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81.
82.
W W Just O Leon G Werner 《Hoppe-Seyler's Zeitschrift für physiologische Chemie》1974,355(12):1565-1568
83.
Werner Ulbricht 《European biophysics journal : EBJ》1974,1(1):1-16
Ionic channels are discrete sites at which the passive movement of ions takes place during nervous excitation. Three types of channels are distinguished. 1. Leakage channels that are permanently open to various cations. 2. Na channels that open promptly on depolarization but slowly close again (inactivate) on sustained depolarization and that are predominantly permeable to Na+ ions. 3. K channels that on depolarization open after some delay but stay open and that are mainly passed by K+ ions. The selectivity sequence of the Na channels of the squid axon (or frog nerve) is as follows: Na+ ≈ Li+>(T1+)>NH+ 4?K+> Rb+, Cs+; that of K channels is: (T1+)>K+>Rb+>NH+ 4?Na+, Cs+, Na channels are selectively blocked by tetrodotoxin (TTX) or saxitoxin (STX), K channels by tetraethylammonium ions (TEA). Either channel type is reversibly blocked when one drug molecule binds to one site per channel, the equilibrium dissociation constant of these reactions being about 3×10?9 MTTX (or STX) and 4×10?4 M TEA, respectively. Because of their specificity and high affinity, TTX and STX are used to “titrate” the Na channels whose density appears to be of the order of 100/Μm2. The “gates” of the channels operate as a function of potential and time but independent of the permeating ion species. Drugs (e.g. veratridine) and enzymes (e.g. pronase, applied intraaxonally) cause profound changes in the gating function of the Na channels without influencing their selectivity. This points to separate structures for gating and ion discrimination. The latter is thought to be, in part, brought about by a “selectivity filter” of which detailed structural ideas exist. Recent experiments suggest that the gates of the Na channels are controlled by charged particles moving within the membrane under the influence of the electrical field. 相似文献
84.
85.
Summary The expression of a newly transferred lacZ
+ gene in lacZ recipients carrying various mutations in the recA and recB genes was studied by measuring the rates of induced synthesis of -galactosidase in zygotes formed after mating with either F or Hfr donors. The ability to synthesize -galactosidase decreases with time in both recA and recB zygotes when the lacZ
+ gene is transferred from an Hfr donor, but not when the lacZ gene is transferred from an F donor. There is no such inactivation of the newly transferred lacZ
+ gene in Rec+ zygotes. We conclude that the functioning of the transferred DNA is progressively inactivated in rec recipients unless the DNA is contained in an episome such as F. 相似文献
86.
Pearl Horn Cooper Irvin N. Hirshfield Werner K. Maas 《Molecular & general genetics : MGG》1969,104(4):383-390
Summary Mutants of Escherichia coli K-12 previously isolated in the authors' laboratory have reduced arginyl-tRNA synthetase activity. The mutants fall into two classes. All mutants grow slowly on arginine-free medium. On arginine-supplemented medium some mutants grow at a normal rate (Class I) while others still grow slowly (Class II). Matings were performed to located a Class I and a Class II mutation on the E. coli chromosome map, and on the basis of our results we have assigned both to one locus, argS. 相似文献
87.
88.
89.
Zusammenfassung Auf Kryostat- Gefrierschnitten von nativem und auch von Formaldehydoder Glutaraldehyd-fixiertem nervösem Gewebe entstehen besonders über der weißen Substanz Cholesterinkristalle in oft dichten Lagen, wenn die trockenen Schnitte bei Raumtemperatur aufbewahrt werden. Ein mehrstündiges Einwirken von gasförmigem Formaldehyd bei 80° C auf die Schnitte sofort nach der Entnahme aus dem Kryostaten verhindert die Kristallbildung. Diese Behandlung der Schnitte beeinflußt die im histologischen Präparat vorhandene Isotopenverteilung nicht und führt auch nicht zu chemographischen Effekten bei autoradiographischen Untersuchungen. Es wird berichtet, bei welchen autoradiographischen Techniken Artefakte durch Cholesterinkristalle zu erwarten sind und welche Auswirkungen diese Kristalle auf die Auswertung von Autoradiogrammen haben.
Cholesterol-crystal deposition in frozen sections of nervous tissue as a cause of artefacts in autoradiograms
Summary In frozen sections of native or formaldehyde or glutaraldehyde fixed nervous tissue, layers of cholesterol crystals have been observed after storage at room temperature, most abundantly in the regions rich in white matter. The formation of the cholesterol depositions could be prevented by treatment of the sections with gaseous formaldehyde at 80° C for several hours, immediately after their removal of the cryostat. The distribution of the radioactive material in the sections was not affected by the formaldehyde treatment. Chemographic effects in the autoradiograms have not been observed. The techniques of autoradiography disturbed by the depositions of cholesterol crystals are reviewed.相似文献
90.
Hfr strains of Escherichia coli K-12 were found capable of accepting a F'lac episome during mating, with a frequency approximating that of F(-) strains. However, the F'lac episome was unable to replicate in the Hfr cells, and was diluted out during the growth of the culture. The lac(+) gene of the episome can be "rescued" by recombination into the host chromosome, as shown by the appearance of variegated recombinant colonies on a lactose-fermentation indicator medium. In recA Hfr strains, however, no lac(+) offspring were obtained in crosses with F'lac donors. The induced synthesis of beta-galactosidase in F'lac(+) x Hfr zygotes was studied. Rates of enzyme synthesis were approximately constant with respect to time as expected from unilinear inheritance of the F'lac episome. However, the rate of synthesis eventually increased, presumably due to integration of the lac(+) gene in some of the zygotes. In F'lac(+) x recA Hfr zygotes the rate of beta-galactosidase synthesis remained constant with respect to time, as expected. 相似文献