全文获取类型
收费全文 | 562921篇 |
免费 | 63115篇 |
国内免费 | 374篇 |
出版年
2016年 | 6442篇 |
2015年 | 8914篇 |
2014年 | 10279篇 |
2013年 | 15233篇 |
2012年 | 16603篇 |
2011年 | 17023篇 |
2010年 | 11483篇 |
2009年 | 10401篇 |
2008年 | 15198篇 |
2007年 | 15753篇 |
2006年 | 14853篇 |
2005年 | 14351篇 |
2004年 | 14319篇 |
2003年 | 13851篇 |
2002年 | 13622篇 |
2001年 | 24062篇 |
2000年 | 24004篇 |
1999年 | 19221篇 |
1998年 | 7027篇 |
1997年 | 7338篇 |
1996年 | 7025篇 |
1995年 | 6551篇 |
1994年 | 6519篇 |
1993年 | 6468篇 |
1992年 | 16532篇 |
1991年 | 16082篇 |
1990年 | 15634篇 |
1989年 | 15329篇 |
1988年 | 14194篇 |
1987年 | 13644篇 |
1986年 | 12371篇 |
1985年 | 12754篇 |
1984年 | 10595篇 |
1983年 | 9081篇 |
1982年 | 7165篇 |
1981年 | 6485篇 |
1980年 | 6164篇 |
1979年 | 10440篇 |
1978年 | 7995篇 |
1977年 | 7571篇 |
1976年 | 7059篇 |
1975年 | 7719篇 |
1974年 | 8414篇 |
1973年 | 8405篇 |
1972年 | 7642篇 |
1971年 | 7102篇 |
1970年 | 6227篇 |
1969年 | 6047篇 |
1968年 | 5475篇 |
1967年 | 4768篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
221.
A newborn child was noted to have an ulcerated lesion on the vertex of her scalp, which was diagnosed as aplasia cutis congenita. As this disorder is relatively rare and frequently misdiagnosed, this case is reported and the relevant literature reviewed. 相似文献
222.
J M Clements S Craig A J Gearing M G Hunter C M Heyworth T M Dexter B I Lord 《Cytokine》1992,4(1):76-82
The murine macrophage inflammatory proteins-1 alpha (MIP-1 alpha) and MIP-1 beta are distinct but closely related cytokines. Partially purified mixtures of the two proteins affect neutrophil function and cause local inflammation and fever. The particular properties of MIP-1 alpha have not been well studied, although it has been identified as being identical to an inhibitor of haemopoietic stem cell growth. We have expressed MIP-1 alpha in yeast cells and purified it to sequence homogeneity. Structural analysis of this biologically active material by circular dichroism and fluorescence spectroscopy confirms that MIP-1 alpha has a very similar secondary and tertiary structure to platelet factor 4 and interleukin 8 with which it shares limited sequence homology. The in-vitro stem cell inhibitory properties have been confirmed using a range of murine progenitor cells including purified bone marrow progenitor cells (FACS-1), the FDCP-mix A4 cell line, and spleen colony forming unit (CFU-S) populations. Plateau levels of inhibition of stem cell growth were achieved using concentrations of 0.15 micrograms/ml MIP-1 alpha. We have also demonstrated that MIP-1 alpha is active in vivo: 5 micrograms of MIP-1 alpha per mouse given as a bolus injection, protects stem cells from subsequent in-vitro killing by tritiated thymidine. MIP-1 alpha was also shown to enhance the proliferation of more committed progenitor granulocyte macrophage-colony forming cells (GM-CFC) in response to granulocyte macrophage-colony stimulating factor (GM-CSF). 相似文献
223.
Based on experiments on agarose gels and tissue, a procedure has been developed which greatly improves the sensitivity and the specifity of the Kossa method for demonstrating calcium in tissue. Tissue calcium is immobilized by acetonic oxalic acid, which simultaneously removes the other sorts of anions capable of precipitating silver ions (e.g. phosphate, carbonate). The resulting submicroscopic grains of calcium oxalate are converted first into silver oxalate then into metallic silver by a treatment with silver nitrate followed by an ultra-violet irradiation (Kossa reaction). These submicroscopic metallic silver grains are enlarged up to microscopic visibility by means of physical development, which makes the staining highly sensitive. Co-staining of the argyrophil sites in the tissue is totally suppressed by various tricks, which render the silver staining selective for calcium. 相似文献
224.
E. Molina Grima J. A. Sánchez Pérez F. García Camacho F. G. Acién Fernández J. M. Fernández Sevilla F. Valdés Sanz 《Biotechnology letters》1994,16(10):1035-1040
Summary Eicosapentaenoic acid (EPA) volumetric productivity from an outdoor chemostat culture ofPhaeodactylum tricornutum UTEX 640 in a 50-l tubular photobioreactor varies with dilution rate, reaching a maximum of 47.8 mg l–1 d–1 at D=0.36 d–1. Continuous culture at high dilution rates' is proposed as the most adequate operating mode to maximize polyunsaturated fatty acid production. 相似文献
225.
226.
227.
228.
Ia I Serkiz A I Lipskaia L B Pinchuk V V Trishin Iu F Kataevski? G N Koval' 《Radiobiologiia》1991,31(5):629-634
Irradiation conditions in which laboratory animals were kept in experimental laboratories of Chernobyl and Kiev after the accident at the Chernobyl A.P.S. are described. The data are presented on the spectral structural and activity of radionuclides in the diet as well as in the organs and tissues of the animals. The radiation loads have been estimated with regard to an external gamma component and the internal one contributed by the incorporated radionuclides. It has been shown that radiation doses received by the animals during their lifetime due to these contributions do not exceed units of cGy. 相似文献
229.
V Ia Gotlib I I Pelevina E F Konoplia A A Al'ferovich A A Konradov 《Radiobiologiia》1991,31(3):318-325
On the basis of their own data and the literature the authors have analyzed the mechanism of biological action of low-level radiation on cells. In studying the harmful effect of gamma-radiation (10-40 cGy) on HeLa cells it was shown that damages occurred in 12 postirradiation generations. Analysis of cell distribution within the colonies has demonstrated that the share of colonies with low cell content and the number of giant cell increase. These data indicate that low-level radiation causes reproductive cell death that can occur not immediately but in later generations. 相似文献
230.