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991.
Arabidopsis AtGSTF2 is regulated by ethylene and auxin, and encodes a glutathione S-transferase that interacts with flavonoids 总被引:1,自引:0,他引:1
Smith AP Nourizadeh SD Peer WA Xu J Bandyopadhyay A Murphy AS Goldsbrough PB 《The Plant journal : for cell and molecular biology》2003,36(4):433-442
Expression of the Arabidopsis glutathione S-transferase (GST) gene AtGSTF2 is induced by several stimuli, but the function of this GST remains unknown. We demonstrate that AtGSTF2 expression is also induced by glutathione, paraquat, copper, and naphthalene acetic acid (NAA) via a mechanism independent of ethylene perception, as determined by analysis of the ethylene-insensitive etr1 mutant. Deletion analyses identified two promoter regions important for regulation of AtGSTF2 expression in response to several of these inducers. Previous studies have suggested that AtGSTF2 interacts with indole-3-acetic acid (IAA) and the auxin transport inhibitor 1-N-naphthylphthalamic acid (NPA). We show that recombinant AtGSTF2 directly binds IAA, NPA, and the artificial auxin NAA. As NPA may act as an endogenous flavonoid regulator of auxin transport, competition between NPA and flavonoids for binding to AtGSTF2 was examined. Both quercetin and kaempferol competed with NPA for AtGSTF2 binding, indicating that all three compounds bind AtGSTF2 at the same site. In transgenic Arabidopsis seedlings, AtGSTF2::GUS expression occurred at the root-shoot transition zone and was induced in this region, as well as at the root distal elongation zone, after treatment with IAA. In wild-type seedlings, AtGSTF2 is localized near the plasma membrane of cells in the root-shoot transition zone. However, both AtGSTF2::GUS expression and localization of AtGSTF2 protein were disrupted in flavonoid-deficient tt4 seedlings. Our results indicate that AtGSTF2 is involved not only in stress responses but also in development under normal growth conditions. 相似文献
992.
The interaction of a variety of aspartic proteinases with a recombinant tomato protein produced in Pichia pastoris was investigated. Only human cathepsin D and, even more potently, proteinase A from Saccharomyces cerevisiae were inhibited. The tomato polypeptide has >80% sequence identity to a previously reported potato inhibitor of cathepsin D. Re-evaluation of the potato inhibitor revealed that it too was more potent (>20-fold) towards yeast proteinase A than cathepsin D and so might be renamed the potato inhibitor of proteinase A. The potency towards yeast proteinase A may reflect a similarity between this fungal enzyme and aspartic proteinases produced by fungal pathogens which attack tomato and/or potatoes. 相似文献
993.
994.
Christian Elabd Wendy Cousin Robert Y. Chen Marc S. Chooljian Joey T. Pham Irina M. Conboy Michael J. Conboy 《The Journal of cell biology》2013,203(1):73-85
Asymmetry of cell fate is one fundamental property of stem cells, in which one daughter cell self-renews, whereas the other differentiates. Evidence of nonrandom template segregation (NRTS) of chromosomes during asymmetric cell divisions in phylogenetically divergent organisms, such as plants, fungi, and mammals, has already been shown. However, before this current work, asymmetric inheritance of chromatids has never been demonstrated in differentiating embryonic stem cells (ESCs), and its molecular mechanism has remained unknown. Our results unambiguously demonstrate NRTS in asymmetrically dividing, differentiating human and mouse ESCs. Moreover, we show that NRTS is dependent on DNA methylation and on Dnmt3 (DNA methyltransferase-3), indicating a molecular mechanism that regulates this phenomenon. Furthermore, our data support the hypothesis that retention of chromatids with the “old” template DNA preserves the epigenetic memory of cell fate, whereas localization of “new” DNA strands and de novo DNA methyltransferase to the lineage-destined daughter cell facilitates epigenetic adaptation to a new cell fate. 相似文献
995.
996.
Doering CJ Kisilevsky AE Feng ZP Arnot MI Peloquin J Hamid J Barr W Nirdosh A Simms B Winkfein RJ Zamponi GW 《The Journal of biological chemistry》2004,279(28):29709-29717
The modulation of N-type calcium channels is a key factor in the control of neurotransmitter release. Whereas N-type channels are inhibited by Gbetagamma subunits in a G protein beta-isoform-dependent manner, channel activity is typically stimulated by activation of protein kinase C (PKC). In addition, there is cross-talk among these pathways, such that PKC-dependent phosphorylation of the Gbetagamma target site on the N-type channel antagonizes subsequent G protein inhibition, albeit only for Gbeta(1)-mediated responses. The molecular mechanisms that control this G protein beta subunit subtype-specific regulation have not been described. Here, we show that G protein inhibition of N-type calcium channels is critically dependent on two separate but adjacent approximately 20-amino acid regions of the Gbeta subunit, plus a highly conserved Asn-Tyr-Val motif. These regions are distinct from those implicated previously in Gbetagamma signaling to other effectors such as G protein-coupled inward rectifier potassium channels, phospholipase beta(2), and adenylyl cyclase, thus raising the possibility that the specificity for G protein signaling to calcium channels might rely on unique G protein structural determinants. In addition, we identify a highly specific locus on the Gbeta(1) subunit that serves as a molecular detector of PKC-dependent phosphorylation of the G protein target site on the N-type channel alpha(1) subunit, thus providing for a molecular basis for G protein-PKC cross-talk. Overall, our results significantly advance our understanding of the molecular details underlying the integration of G protein and PKC signaling pathways at the level of the N-type calcium channel alpha(1) subunit. 相似文献
997.
Wendy J. Williams B. Büdel H. Reichenberger N. Rose 《Biodiversity and Conservation》2014,23(7):1827-1844
This research was conducted in the northern Australian savannah at Boodjamulla National Park where cyanobacterial crusts dominate the soil and rock surfaces in between tussock grasses. It is widely accepted that terrestrial cyanobacteria are drought tolerant and rapidly recommence photosynthesis once moisture is available. Initial tests at the research site indicated that cyanobacteria did not respond to rehydration during the dry season, even after several days. We hypothesised that resurrection had not taken place and new growth from survival cells had to take place during the follow-up wet season. To further understand the desiccation–resurrection processes we tested photosystem II (PSII) responses both during the dry and wet seasons. In the 2009 dry season after 125 days without rain, crust samples were regularly rehydrated. Over the 10 day trial cyanobacteria did not recover PSII activity or CO2-uptake. Although new colonies of Nostoc grew other cyanobacteria remained inactive, even though liverworts and lichens in the same crusts had responded within 24 h. Dry season cyanobacterial crusts were collected in 2010 then reintroduced into their natural environment and exposed to rainfall during the 2011 wet season. Within 24 h PSII in cyanobacteria from a range of crust types had resurrected and CO2-uptake was verified, although different crust types responded at significantly different rates. These are the first studies that have demonstrated that PSII does not respond to rainfall during the dry season and cyanobacterial function appears controlled by other environmental conditions. It is likely that mass extracellular polysaccharide (EPS) production during the wet season, once dry, protects cyanobacteria from premature resurrection in the dry season. We propose that EPS regulates moisture penetration, thus the resurrection of PSII at the onset of the wet season, at which time moisture and humidity alters the rheological properties of EPS permitting rehydration. 相似文献
998.
Subcellular targeting of human interleukin-10 in plants 总被引:2,自引:0,他引:2
Menassa R Kennette W Nguyen V Rymerson R Jevnikar A Brandle J 《Journal of biotechnology》2004,108(2):179-183
The utility of plants for the production of a wide range of recombinant proteins is now clearly established. However, the challenge remains to produce these proteins at sufficient concentrations for extraction to be economically feasible. In this paper, we have investigated the ability of plant cells to accumulate the human interleukin-10 (IL-10) protein targeted to chloroplasts and mitochondria. We found that IL-10 accumulates in chloroplasts only if a 6 x His tag is added at the C-terminus of the protein. The hexapeptide may provide protection from degradation. Conversely, the IL-10 protein does not accumulate in mitochondria. Analysis of the chloroplast-targeted IL-10 protein revealed only monomeric IL-10 and limited biological activity in in vitro cell assays. 相似文献
999.
1000.
Xiaoyang Ling Erika Spaeth Ye Chen Yuexi Shi Weiguo Zhang Wendy Schober Numsen Hail Jr Marina Konopleva Michael Andreeff 《PloS one》2013,8(3)
CXCR4, the receptor for stromal-derived factor-1, is reportedly involved in breast carcinogenesis. However, the mechanisms through which CXCR4 contributes to breast cancer cell growth and metastases are poorly understood. In this study, we examined the putative in vitro and in vivo anti-cancer effects of the specific CXCR4 inhibitor AMD3465. Here, we report that AMD3465 triggers a reduction in breast cancer cell invasiveness in vitro, and promotes marked changes in oncogenic signaling proteins including a reduction in STAT3, JAK2, AKT, and CXCR4 phosphorylation and the reduced expression of GSK3 and cMYC. Using three breast cancer cell lines as murine syngeneic immunocompetent breast cancer models, we found that AMD3465 inhibited breast tumor formation and reduced tumor cell metastases to the lung and liver. Furthermore, treatment with AMD3465 significantly reduced the infiltration of myeloid CD11b positive cells at the aforementioned metastatic sites as well as the spleen implying this agent could regulate the formation of the tumor microenvironment and conceivably the premetastatic niche. In conclusion, our studies suggest that AMD3465 inhibits breast cancer growth and metastases by acting on tumor cells as well as immune cells that constitute the tumor microenvironment. This process appears to be regulated, at least in part, through the modulation of oncogenic signaling that includes the STAT3 pathway. Thus, CXCR4 could be a novel target for breast cancer therapy. 相似文献