全文获取类型
收费全文 | 6237篇 |
免费 | 616篇 |
国内免费 | 4篇 |
出版年
2023年 | 25篇 |
2022年 | 61篇 |
2021年 | 107篇 |
2020年 | 81篇 |
2019年 | 91篇 |
2018年 | 110篇 |
2017年 | 96篇 |
2016年 | 194篇 |
2015年 | 290篇 |
2014年 | 321篇 |
2013年 | 377篇 |
2012年 | 442篇 |
2011年 | 463篇 |
2010年 | 292篇 |
2009年 | 282篇 |
2008年 | 317篇 |
2007年 | 347篇 |
2006年 | 344篇 |
2005年 | 304篇 |
2004年 | 287篇 |
2003年 | 269篇 |
2002年 | 254篇 |
2001年 | 127篇 |
2000年 | 109篇 |
1999年 | 110篇 |
1998年 | 83篇 |
1997年 | 53篇 |
1996年 | 52篇 |
1995年 | 55篇 |
1994年 | 39篇 |
1993年 | 54篇 |
1992年 | 66篇 |
1991年 | 79篇 |
1990年 | 72篇 |
1989年 | 64篇 |
1988年 | 54篇 |
1987年 | 47篇 |
1986年 | 53篇 |
1985年 | 35篇 |
1984年 | 43篇 |
1983年 | 42篇 |
1982年 | 42篇 |
1981年 | 36篇 |
1980年 | 19篇 |
1979年 | 18篇 |
1978年 | 20篇 |
1977年 | 18篇 |
1972年 | 14篇 |
1971年 | 17篇 |
1970年 | 13篇 |
排序方式: 共有6857条查询结果,搜索用时 171 毫秒
131.
132.
Myocardial intracellular pH regulation during chloride depletion 总被引:1,自引:0,他引:1
133.
E C Greco W E Fordyce F Gonzalez P Reischl F S Grodins 《Journal of applied physiology (Bethesda, Md. : 1985)》1978,45(1):109-114
Ventilatory responses to CO2 inhalation and CO2 infusion were compared in the awake dog. The CO2 was introduced directly into the systemic venous blood via a membrane gas exchanger in a femoral arteriovenous shunt circuit, and the extracorporeal blood flow, QX, was maintained constant at one of two rates: low, 0.5 l/min; or high, 2.0 l/min. A total of 13 experiments was performed in four dogs comprising 50 control and 25 inhalation and infusion observations at each of the two flow rates. Comparison of CO2-response curve slopes, S = delta V E/delta PaCO2, between CO2 inhalation and infusion showed no significant difference either within or between flow rates. The mean value of S for all conditions was 1.88 l/min per Torr with a 95% confidence interval of 1.66 -2.14. An independent additive ventilatory drive amounting to 28% of low-flow control VE was found at the highflow rate. We conclude that at constant blood flow the responses to both CO2 inhalation and infusion are hypercapnic and not significantly different. 相似文献
134.
A simple procedure for resolution of Escherichia coli RNA polymerase holoenzyme from core polymerase. 总被引:32,自引:0,他引:32
The association constant, KA, for myosin subfragment-1 binding to actin was measured as a function of ionic strength [KCl, LiCl, and tetramethylammonium chloride (TMAC)]and temperature by the method of time-resolved fluorescence depolarization. The following thermodynamic values were obtained from solutions of 0.20 × 10?6m S-1, 1.00 × 10?6m actin in 0.15 m KCl, pH 7.0, at 25 °C: ΔG ° = ?39 ± 1 kJ M?1, ΔH0 = 44 ± 2 kJ M?1 and . For measurements in KCl (0.05 to 0.60 m), In . Thus, the binding is endothermic and strongly inhibited by high ionic strength. When KCl was replaced by LiCl or TMAC the ionic effects on the binding were cation specific. The nature of actin-(S-1) binding in the rigor state is discussed in terms of these results. 相似文献
135.
136.
137.
Calmodulin copurifies with platelet plasma membranes isolated by glycerol-induced lysis and density gradient centrifugation. These membranes also bind 125I-labeled calmodulin in vitro in the presence of Ca2+. Binding is largely reduced by replacing Ca2+ by Mg2+ or by addition of an excess unlabeled calmodulin. The specific component of binding is saturable, with an apparent Kd of 27 nM and a maximum of 15.9 pmol binding sites per mg of membrane protein. This is equivalent to approx. 4100 binding sites per platelet. Binding was inhibited by addition of phenothiazines, a group of calmodulin antagonists. Half-maximal inhibition was attained with approx. 20 μM trifluoperazine or 50 μM chlorpromazine. In contrast, chlorpromazine-sulfoxide which is inactive towards calmodulin, did not affect the binding. Calmodulin binding polypeptides of the plasma membrane were identified by a gel-overlay technique. A major calmodulin-binding component of molecular weight 149 000 was detected. Binding to this band was Ca2+-dependent and inhibited by chlorpromazine. The molecular weight of this polypeptide is similar to that of glycoprotein I and also that of the red cell (Ca2+ + Mg2+)-stimulated ATPase, which is known to bind calmodulin. The possible role of calmodulin in platelet activation is analysed. 相似文献
138.
William R. Cullen Ann E. Erdman Barry C. McBride A.Wendy Pickett 《Journal of microbiological methods》1983,1(5):297-303
Candida humicola acts on benzenearsonic acid to produce dimethylphenylarsine, which was identified by mass spectroscopy following the chemofocusing of the volatile metabolite onto a mercuric chloride impregnated filter. The same technique established that trimethylarsine is the volatile metabolic product obtained from C. humicola treated with 4-NH2-2-OHC6H3AsO(OH)2 and (CH3)3AsO. Arsanilic acid, 4-NH2C6H4AsO(OH)2, is not metabolized to a volatile arsine. 相似文献
139.
Purification, characterization and some properties of diacetyl(acetoin) reductase from Enterobacter aerogenes 总被引:1,自引:0,他引:1
A new method, faster, milder and more efficient than the one previously described [Bryn, K., Hetland, O. & Stormer, F. C. (1971) Eur. J. Biochem, 18, 116-119], for purification of diacetyl(acetoin) reductase from Enterobacter aerogenes is proposed. The experiments carried out with the electrophoretically pure preparations obtained by this procedure show that the enzyme (a) produces L-glycols from the corresponding L-alpha-hydroxycarbonyls by reversible reduction of their oxo groups and also reduces the oxo group of uncharged alpha-dicarbonyls converting them into L-alpha-hydroxycarbonyls, and (b) is specific for NAD. This is a new enzyme for which we suggest the systematic name of L-glycol: NAD+ oxidoreductase and the recommended name of L-glycol dehydrogenase(NAD). The molecular mass, pI, affinity for substrates and pH profiles of this enzyme are also described. 相似文献
140.
Lawrence G. Miller Monica Lumpkin Wendy R. Galpern David J. Greenblatt Richard I. Shader 《Journal of neurochemistry》1991,56(4):1241-1247
The irreversible protein-modifying reagent N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ) was used to investigate binding site characteristics on the gamma-aminobutyric acidA (GABAA) receptor complex. In vitro, preincubation with EEDQ led to a concentration-dependent decrease in receptor number for benzodiazepine, t-butylbicyclophosphorothionate (TBPS), and GABA binding sites in cerebral cortex. The effect was maximal at the highest concentration of EEDQ used (10(-4) M) and was greatest for the benzodiazepine site. Pretreatment of membranes with the benzodiazepine antagonist Ro 15-1788, 1 or 10 microM, or the agonist lorazepam, 10 microM, largely prevented the effects of EEDQ. Scatchard analysis indicated no effect of EEDQ, 10(-4) M, on apparent affinity, but a decrease in receptor density for each site. Administration of EEDQ to mice, 12.5 mg/kg i.p., led to a substantial (55-65%) decrease in number of benzodiazepine binding sites in cortex after 4 h. Slightly smaller changes were observed for TBPS and GABA binding. No changes were observed in apparent affinity at any site. Prior administration of Ro 15-1788, 5 mg/kg, prevented the effect of EEDQ on benzodiazepine binding. Density of benzodiazepine binding sites gradually recovered over time, and receptor density returned to control values by 96 h after EEDQ injection. Number of binding sites in cortex for TBPS and GABA also increased over time after EEDQ. Benzodiazepine sites in cerebellum were decreased proportionally to cortex after EEDQ, and increased over a similar time course. Function of the GABAA receptor in chloride uptake in cortex was markedly reduced (65%) by EEDQ.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献