Characterization of cis-acting elements in light regulation of the nuclear gene encoding the A subunit of chloroplast isozymes of glyceraldehyde-3-phosphate dehydrogenase from Arabidopsis thaliana.

We have characterized cis-acting elements involved in light regulation of the nuclear gene (GapA) encoding the A subunit of chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in Arabidopsis thaliana. Our results show that a 1.1-kb promoter fragment of the GapA gene is sufficient to confer light inducibility and organ specificity in transgenic Nicotiana tabacum (tobacco) plants, using the beta-glucuronidase gene of Escherichia coli as the reporter gene. Deletion analysis indicates that the -359 to -110 bp region of the GapA gene is necessary for light responsiveness. Within this region there are three copies of a decamer repeat (termed the Gap box) having the consensus sequence 5'-CAAATGAA(A/G)A-3', which has not been characterized in the promoter regions of other light-regulated genes. A deletion (to -247) producing loss of one copy of these elements from the GapA promoter reduces light induction by two- to threefold compared with a promoter deletion (to -359) with all three Gap boxes present, while deletion of all three Gap boxes (to -110) abolishes light induction completely. Gel mobility shift experiments using tobacco nuclei as the source of nuclear proteins show that GapA promoter fragments that contain these repeats bind strongly to a factor in the nuclear extract and that binding can be abolished by synthetic competitors consisting only of a monomer or dimer of the Gap box. Furthermore, a trimer, dimer, and monomer of the Gap box show binding activity and, like the authentic GapA promoter-derived probes, show binding activities that are correlated with Gap box copy number. These results strongly suggest that these repeats play important roles in light regulation of the GapA gene of A. thaliana.     

含金地质体表生带中主要微生物类群的研究

通过对16个含金矿区主要微生物类群的分布、生态结构的研究,发现不同类型的金矿具有不同的微生物类群.金矿矿石与金矿酸水中的自养硫细菌和异养菌分布具有明显差异,酸性水中含自养硫细菌较多,含异养菌极少;金矿氧化带中的异氧菌一般多于自氧硫细菌,其优势菌以芽孢杆菌属中蜡状芽孢杆菌为主.研究结果揭示了金矿中存在的主要微生物类群,对阐明表生金的形成、成岩和蚀变作用具有一定的意义,对发掘生物湿法冶金菌种资源具有重要作用.     

Stomatal sensitivity to abscisic acid following water deficit stress

Short-and medium-term stresses (1 and 24 h, respectively) wereapplied to detached leaves of Commelina communis L., resultingin both cases in a final leaf cell water potential (     

Nanometric design of extraordinary hydrophobic-induced pKa shifts for aspartic acid: Relevance to protein mechanisms

Commonly a key element enabling proteins to function is an amino acid residue or residues with functional side chains having shifted pKa values. This article reports the results on a set of protein-based polymers (model proteins) that exhibit hydrophobic folding and assembly transitions, and that have been designed for the purpose of achieving large hydrophobic-induced pKa shifts by selectively replacing Val residues by Phe residues. The high molecular weight polypentapeptides, actually poly (tricosapeptides) with six varied pentamers in fixed sequence, were designed and synthesized to have the same amino acid compositions but different proximities between a single aspartic acid residue and 5 Phe residues per 30 residues. With the 5 Phe residues distal from the Asp residue, the observed pKa shift was 2.9 when compared to the Val-containing reference. With the 5 Phe residues within 1 nm of the Asp residue, the pKa shift was 6.2. This represents a free energy of interaction of 8 kcal/moles. To our knowledge, this is the largest pKa shift documented for an Asp residue in a polypeptide– or protein–water system. Data are reviewed that do not support the usual electrostatic arguments for pKa shifts of charge–charge repulsion and/or unfavorable ion self-energies arising from displacement of water by hydrophobic moieties, but rather the data are interpreted to indicate the presence of an apolar–polar repulsive free energy of hydration, which results from a potentially highly cooperative competition between apolar and polar species for hydration. © 1994 John Wiley & Sons, Inc.     

广东种子植物区系地理成分研究

广东种子植物区系共有２１９科１４３４属４９８６种。其中：①热带区系成分占有较大的比例,约占非世界属的６２．３％,表明广东区系受到热带区系的强烈渗透,次之为亚热带（至亚热带山地）成分约占３２．２％,及中国特有分布占５．６％;②以单型属（７２属）,单种属（５８５属）和寡种属（５６２属）占绝对优势,共占８５．０％,充分显示了广东区系的古老性和过渡性特点;③从区系组成和植被结构的特征成分来看,以华夏植物区系成分为主,包括：亚热带分布,亚热带山地分布,中国特有分布,东亚—北美间断分布,及一些亚洲热带分布中以亚热带为主的属：④在这些属中有东亚特有属１３２属,中国特有属７５属以及华南—西南—中南半岛特有属６８属等;这些属的存在毫无疑问地说明,广东区系是华夏植物区系的核心地区之一,同时也显示了热带区系与亚热带区系有着不可分隔的统一性。     

Systematic classification of Asian colobines

In order to study the differentiation of Asian colobines, fourteen variables were analysed in one way, on 123 skulls, includingRhinopithecus, Presbytis, Presbytiscus, Pygathrix, andNasalis with both cluster and differentiated functions tests. Information on paleoenvironment changes in China and South-East Asia since late Tertiary have been used to examine the influences of migratory habits and the distribution range in Asian colobines. The cladogram among different Asian colobines genera was made from the results of various analysis. Some new points or revisions were suggested: 1. Following the second migratory way, ancient species of Asian colobines perhaps passed through Xizang along the northern bank of Tethis sea and Heng-Duan Shan regions, across Yunnan into Vietnam, since the ancient continent between Yunnan and Xizang was already located in on eastern bank of Tethis sea. Thus, during the evolution, Asian colobines must have had two original centres, i.e. “Sundaland” and Heng-Duan Shan Chinese regions; 2. Pygatrix possesses a lot of cranial features more similar toPresbytiscus than toRhinopithecus. The small difference from the modification combinesPygatrix with other two genera as shown by Groves (1970), but it is better to putPygatrix andPresbytiscus together as one genus; 3.Nasalis (2n=48) may be the most primitive genus within Asian colobines. Some features shared withRhinopithecus, for example body size, terrestrial activities, limb proportion etc. ...seem to be considered as a common inheritance of symlesiomorphus characters; 4.Rhinopithecus, with reference to cranioface and cranium or to its origin, is a special genus of Asian colobine. It may represent the highest level of evolutionary position within various genera (Peng et al., 1985).     

祁连山地区植被特征及其分布规律

分析和讨论了祁连山地区主要植被类型及其分布特征。祁连山地区随着青藏高原的强烈隆升表现为整体抬升,植被具有明显的生态地理边缘效应特征和高原地带性规律。该区植被虽然受到四周的较大影响,但各类高寒植被占有绝对优势,表现出与青藏高原植被整体明显的相似性和广泛的一致性。另一方面,本区植被也有其特殊性及与高原面存在一些差异。因此,建议把祁连山地区做为青藏高原植被区的次一级独立单元     

质体醌重组的D1／D2／Cytb559复合物的荧光衰减动力学和光破坏作用

光系统Ⅱ反应中心Ｄ１／Ｄ２／Ｃｙｔｂ５５９ 在分离纯化过程中失去了电子受体ＱＡ 和ＱＢ,人工合成的质体醌可以与Ｄ１／Ｄ２／Ｃｙｔｂ５５９ 复合物发生重组。癸基质体醌（ＤＰＱ）与Ｄ１／Ｄ２／Ｃｙｔｂ５９９ 复合物的重组导致该复合物的荧光强度下降及发射光谱蓝移,同时两个与光化学活性相关的长寿命（２４ ｎｓ和７３ ｎｓ）荧光衰减组分占整个荧光的百分数下降,这些结果表明ＤＰＱ作为Ｐｈｅｏ－ 的电子受体,限制了Ｐ６８０＋ ·Ｐｈｅｏ－ 的电荷重组。ＤＰＱ 的加入对Ｄ１／Ｄ２／Ｃｙｔｂ５５９复合物中Ｃｈｌａ 分子的光破坏敏感性影响不大,但β－胡萝卜素在加入ＤＰＱ 之后可以被光照破坏,这个过程可能与β－胡萝卜素的生理功能相关。     

人肾液泡型H~＋_－ATPase 58kD亚基基因的表达

在大肠杆菌中表达了人肾液泡型Ｈ￣＋－ＡＴＰａｓｅ５８ｋＤ亚基的基因,利用聚合酶链式反应（ＰＣＲ）得到了５８ｋＤ亚基的编码片段．直接将ＰＣＲ产物连接到ＰＥＴ载体上表达．ＳＤＳ聚丙烯酰胺凝胶电泳和蛋白质印迹分析表明５８ｋＤ亚基的基因得到高效表达．表达产物可达细菌细胞质蛋白的５０％．