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91.
Kong Wen-Hua Zhao Rong Zhou Jun-Bo Wang Fang Kong De-Guang Sun Jian-Bin Ruan Qiong-Fang Liu Man-Qing 《中国病毒学》2020,35(6):752-757
Virologica Sinica - The immense patient number caused by coronavirus disease 2019 (COVID-19) global pandemic brings the urge for more knowledge about its immunological features, including the... 相似文献
92.
该文探讨了SIRT3激动剂(Honokiol,HKL)对乙肝病毒(Hepatitis B virus,HBV)转录和复制的影响。培养HepG2-NTCP和人原代肝细胞(primary human hepatocytes,PHH),感染HBV颗粒后,用Honokiol(5μmol/L、10μmol/L、20μmol/L)处理细胞后继续培养10天,通过荧光定量PCR检测细胞内HBV DNA、cccDNA和HBV RNAs水平,Southern blot实验进一步检测胞内HBV DNA水平。构建SIRT3-KO细胞,检测敲除SIRT3后,Honokiol对细胞内HBV DNA、cccDNA和HBV RNAs的影响。通过小鼠尾静脉高压注射pCMV-KRAB-Cre质粒和precccDNA质粒构建持续感染小鼠模型,一周后腹腔注射Honokiol持续20天。荧光定量PCR检测小鼠血清中HBV DNA拷贝数,肝组织内HBV DNA、cccDNA和HBV RNAs水平。结果表明,Honokiol浓度依赖性地抑制HepG2-NTCP和PHH细胞内HBV DNA以及HBV RNAs水平,此外,Honokiol可以降低cccDNA的转录活性;敲除SIRT3后,Honokiol不能发挥抗病毒作用;小鼠模型中,Honokiol能够降低血清中HBV DNA和肝组织内HBV DNA拷贝数,以及能够显著抑制肝组织内HBV RNAs水平和cccDNA的转录活性。该研究结果表明,Honokiol能够抑制乙肝病毒转录和复制。 相似文献
93.
Yingfan Cai Xiaoyan Cai Qinglian Wang Ping Wang Yu Zhang Chaowei Cai Yanchao Xu Kunbo Wang Zhongli Zhou Chenxiao Wang Shuaipeng Geng Bo Li Qi Dong Yuqing Hou Heng Wang Peng Ai Zhen Liu Feifei Yi Minshan Sun Guoyong An Jieru Cheng Yuanyuan Zhang Qian Shi Yuanhui Xie Xinying Shi Ying Chang Feifei Huang Yun Chen Shimiao Hong Lingyu Mi Quan Sun Lin Zhang Baoliang Zhou Renhai Peng Xiao Zhang Fang Liu 《Plant biotechnology journal》2020,18(3):814-828
94.
95.
Zaiyu Zheng Jinxian Yang Junqing Ge Hongshu Chi Bin Chen Qinmei Fang Hui Gong 《Cell biology international》2020,44(3):808-820
In the present study, a new hepatic tissue‐origin cell line from European eel Anguilla anguilla has been developed and characterized. This cell line designated EL has been maintained in Leibovitz L‐15 supplemented with 10% fetal bovine serum over 72 months, and subcultured more than 90 times. The EL cell line consisted predominantly of fibroblast‐like cells, which could survive over 100 days in vitro, and could grow at 15–32°C. The optimum temperature for growth was 27°C. The chromosome analysis revealed a modal diploid karyotype of 2n = 38. The origin of this cell line was confirmed by the 18S recombinant (r)RNA sequencing. The susceptibility test indicated significant cytopathic effects in the EL cells with regard to the Rana grylio virus and the Herpesvirus anguillae. The viral replication was confirmed by transmission electron microscopy and polymerase chain reaction analysis. Following poly (I:C) exposure, the expression levels of the immune‐related molecules interferon regulatory factor‐7 (irf7) and transforming growth factor‐β (TGF‐β) were downregulated in EL cells, whereas the expression levels of the rf3 and the cytochrome P450 (CYP450) were upregulated. All four genes were significantly upregulated following inflammation by lipopolysaccharide (LPS). These data suggested the application of EL cell line for viral identification, as well as for immunodiagnosis and pharmacological targeting. 相似文献
96.
Inamur Rahman Lina Fang Zhang Wei Xiaodong Zheng Lei Huang 《Preparative biochemistry & biotechnology》2020,50(6):598-606
AbstractHuman basic fibroblast growth factor (hbFGF) is involved in a wide range of biological activities that affect the growth, differentiation, and migration. Due to its wound healing effects and therapy, hbFGF has the potential as therapeutic agent. Therefore, large-scale production of biologically active recombinant hbFGF with low cost is highly desirable. However, the complex structure of hbFGF hinders its high-level expression as the soluble and functional form. In the present study, an efficient, cost-effective, and scalable method for producing recombinant hbFGF was developed. The modified collagen-like protein (Scl2-M) from Streptococcus pyogenes was used as the fusion tag for producing recombinant hbFGF for the first time. After optimization, the expression level of Scl2-M-hbFGF reached approximately 0.85?g/L in the shake flask and 7.7?g/L in a high cell-density fermenter using glycerol as a carbon source. Then, the recombinant Scl2-M-hbFGF was readily purified using one-step acid precipitation and the purified Scl2-M-hbFGF was digested with enterokinase. The digested mixture was further subject to ion-exchange chromatography, and the final high-purity (96%) hbFGF product was prepared by freeze-drying. The recovery rate of the whole purification process attained 55.0%. In addition, the biological activity of recombinant hbFGF was confirmed by using L929 and BALB/c3T3 fibroblasts. Overall, this method has the potential for large scale production of recombinant hbFGF. 相似文献
97.
98.
99.
Guo Kaiqiang Cao Yin Li Zan Zhou Xiaoxiao Ding Rong Chen Kejing Liu Yan Qiu Yingkun Wu Zhen Fang Meijuan 《Amino acids》2020,52(5):793-809
Amino Acids - Glycine plays a key role in rapidly proliferating cancer cells such as A549 cells. Targeting glycine metabolism is considered as a potential means for cancer treatment. However, the... 相似文献
100.
Mammalian Genome - Prostate cancer, the second most common cancer among male adults, affects millions globally. We sought to investigate the expression and contribution of Eukaryotic translation... 相似文献