全文获取类型
收费全文 | 95678篇 |
免费 | 8484篇 |
国内免费 | 7398篇 |
出版年
2024年 | 123篇 |
2023年 | 1047篇 |
2022年 | 1932篇 |
2021年 | 4543篇 |
2020年 | 3117篇 |
2019年 | 3772篇 |
2018年 | 3732篇 |
2017年 | 2712篇 |
2016年 | 3709篇 |
2015年 | 5722篇 |
2014年 | 6652篇 |
2013年 | 7300篇 |
2012年 | 8539篇 |
2011年 | 7881篇 |
2010年 | 4818篇 |
2009年 | 4242篇 |
2008年 | 5121篇 |
2007年 | 4536篇 |
2006年 | 3943篇 |
2005年 | 3344篇 |
2004年 | 2961篇 |
2003年 | 2626篇 |
2002年 | 2326篇 |
2001年 | 1958篇 |
2000年 | 1870篇 |
1999年 | 1731篇 |
1998年 | 1024篇 |
1997年 | 943篇 |
1996年 | 934篇 |
1995年 | 855篇 |
1994年 | 769篇 |
1993年 | 613篇 |
1992年 | 884篇 |
1991年 | 711篇 |
1990年 | 649篇 |
1989年 | 590篇 |
1988年 | 458篇 |
1987年 | 386篇 |
1986年 | 359篇 |
1985年 | 314篇 |
1984年 | 234篇 |
1983年 | 215篇 |
1982年 | 129篇 |
1981年 | 129篇 |
1980年 | 91篇 |
1979年 | 153篇 |
1978年 | 85篇 |
1977年 | 97篇 |
1975年 | 113篇 |
1974年 | 118篇 |
排序方式: 共有10000条查询结果,搜索用时 343 毫秒
11.
Shira Weingarten-Gabbay Susan Klaeger Siranush Sarkizova Leah R. Pearlman Da-Yuan Chen Kathleen M.E. Gallagher Matthew R. Bauer Hannah B. Taylor W. Augustine Dunn Christina Tarr John Sidney Suzanna Rachimi Hasahn L. Conway Katelin Katsis Yuntong Wang Del Leistritz-Edwards Melissa R. Durkin Christopher H. Tomkins-Tinch Pardis C. Sabeti 《Cell》2021,184(15):3962-3980.e17
- Download : Download high-res image (225KB)
- Download : Download full-size image
12.
A microcomputer mainframe linked system is described which allows video camera data capture and storage of one-dimensional whole-cell protein electrophoresis gel images, processing of normalized traces to produce a similarity matrix, and analysis of the matrix using the commercial cluster analysis program CLUSTAN. A new similarity coefficient is introduced which takes into account both band position and intensity. Forty-five strains of Haemophilus influenzae, including the eight biotypes and six serotypes, were analyzed using this system. Results demonstrated groupings which are consistent with known genetic relationships. 相似文献
13.
The genome of Mus domesticus has multiple genes of the alpha 1-acid glycoprotein (AGP). Two cDNA clones were identified corresponding to AGP-1 and AGP-2. Moreover, two alleles of AGP-1 exist in inbred mice. The genomic DNA of the AGP-2 gene has been cloned and studied. Here we report the genomic organization of three M. domesticus AGP genes, the sequence analysis of the AGP-3 genomic DNA, and the expression of the AGP-3 gene. The major structural differences between AGP-2 and AGP-3 genes are located in introns 1 and 5. The low level of AGP-3 mRNA can be detected by the polymerase chain reaction (PCR). The molecular basis of the low level expression of AGP-3 and the possible classification of AGP-3 as a pseudogene are discussed. 相似文献
14.
Recent studies have revealed an unexpected synergism between two seemingly unrelated protein families: CCN matricellular proteins
and the tumor necrosis factor (TNF) family of cytokines. CCN proteins are dynamically expressed at sites of injury repair
and inflammation, where TNF cytokines are also expressed. Although TNFα is an apoptotic inducer in some cancer cells, it activates
NFκB to promote survival and proliferation in normal cells, and its cytotoxicity requires inhibition of de novo protein synthesis
or NFκB signaling. The presence of CCN1, CCN2, or CCN3 overrides this requirement and unmasks the apoptotic potential of TNFα,
thus converting TNFα from a proliferation-promoting protein into an apoptotic inducer. These CCN proteins also enhance the
cytotoxicity of other TNF cytokines, including LTα, FasL, and TRAIL. Mechanistically, CCNs function through integrin α6β1 and the heparan sulfate proteoglycan (HSPG) syndecan-4 to induce reactive oxygen species (ROS) accumulation, which is essential
for apoptotic synergism. Mutant CCN1 proteins defective for binding α6β1-HSPGs are unable to induce ROS or apoptotic synergism with TNF cytokines. Further, knockin mice that express an α6β1-HSPG-binding defective CCN1 are blunted in TNFα- and Fas-mediated apoptosis, indicating that CCN1 is a physiologic regulator
of these processes. These findings implicate CCN proteins as contextual regulators of the inflammatory response by dictating
or enhancing the cytotoxicity of TNFα and related cytokines. 相似文献
15.
16.
17.
目的 研究严重急性呼吸综合征冠状病毒2(SARS-CoV-2)膜蛋白对宿主细胞mRNA前体(pre-mRNA)3"非翻译区(UTR)加工的影响。方法 本研究以人肺上皮细胞系A549为模型,利用瞬时转染在细胞内过表达SARS-CoV-2膜蛋白;利用RNA-Seq测序技术及生物信息学分析方法,系统性描绘宿主细胞选择性多聚腺苷酸化(alternative polyadenylation,APA)事件;Metascape数据库对发生显著APA变化的基因进行功能富集分析;RT-qPCR验证靶基因3"UTR长度变化;蛋白质免疫印迹(Western blot)检测目的蛋白表达水平。结果 SARS-CoV-2膜蛋白外源表达后宿主细胞内共813个基因发生显著APA变化。GO和KEGG分析显示,差异APA基因广泛参与有丝分裂细胞周期、调节细胞应激等生物过程,涉及病毒感染和蛋白质加工等。从中进一步筛选出AKT1基因,在IGV软件中显示3"UTR延长;RT-qPCR验证AKT1基因的3"UTR长度变化趋势;Western blot结果显示AKT1蛋白磷酸化水平增加。结论 SARS-CoV-2膜蛋白潜在影响宿主pre-mRNA的3"UTR加工,其中参与多种病毒性生物过程的AKT1基因 3"UTR延长,且其编码的蛋白质功能在细胞内被激活。 相似文献
18.
19.
Jin Wei Mia Madel Alfajaro Peter C. DeWeirdt Ruth E. Hanna William J. Lu-Culligan Wesley L. Cai Madison S. Strine Shang-Min Zhang Vincent R. Graziano Cameron O. Schmitz Jennifer S. Chen Madeleine C. Mankowski Renata B. Filler Neal G. Ravindra Victor Gasque Fernando J. de Miguel Ajinkya Patil Huacui Chen Craig B. Wilen 《Cell》2021,184(1):76-91.e13
- Download : Download high-res image (212KB)
- Download : Download full-size image
20.
Yunfan Sun Liang Wu Yu Zhong Kaiqian Zhou Yong Hou Zifei Wang Zefan Zhang Jiarui Xie Chunqing Wang Dandan Chen Yaling Huang Xiaochan Wei Yinghong Shi Zhikun Zhao Yuehua Li Ziwei Guo Qichao Yu Liqin Xu Jia Fan 《Cell》2021,184(2):404-421.e16
- Download : Download high-res image (233KB)
- Download : Download full-size image