微重力组织工程的产生与发展

概述了微重力组织工程的产生与发展趋势。动物细胞培养技术为研究细胞的形态、结构、功能与遗传特性 ,揭示细胞分裂、组织分化、器官组织形成等生命科学领域的基本问题发挥了巨大作用 ;大规模动物细胞培养技术已广泛应用于生物制药、生产疫苗以及生物学诊断试剂。但是 ,常规培养技术无法实现由细胞重建组织这一构想。微重力组织工程利用旋转培养器产生的微重力环境与动物细胞培养技术相结合 ,使组织重建成为可能 ,成为生命科学发展史上的一个新的里程碑。     

Transgenic tall fescue containing the Agrobacterium tumefaciens ipt gene shows enhanced cold tolerance

Embryogenic calli of Festuca arundinacea were transformed with the Agrobacterium tumefaciens isopentenyl transferase (ipt) gene driven by a maize ubiquitin promoter. Tillering ability, levels of chlorophyll a and b, and cold tolerance were greatly increased in the transgenic turfgrass, which resulted in the plants remaining more vigorous and staying green longer under lower temperatures.     

肠易激综合征患者肠道菌群分布和炎症因子及血清NPY、SP、5\|HT水平的变化

目的 探究肠易激综合征(IBS)患者肠道菌群分布、相关炎症因子和血清神经肽Y(NPY)、P物质(SP)、5\|羟色胺(5\|HT)水平的变化。 方法 选择2017年1月至2018年12月我院收治的80例IBS患者为研究对象,入选对象分为腹泻组(IBS\|D组,40例)和便秘组(IBS\|C组,40例)。以40例结肠息肉或胃息肉切除术后复查者作为对照组。检测3组患者肠道菌群数量与定植抗力(B/E值)。利用酶联免疫吸附法(ELISA)检测血清IL\|6、IL\|8、TNF\|α水平及血清NPY、SP、5\|HT水平变化。 结果 IBS\|D组患者肠道双歧杆菌、乳杆菌、类杆菌数量及B/E值均低于对照组(均P结论 IBS会导致患者肠道定植抗力下降,进而发生肠道菌群失调并伴随炎症因子水平与血清NPY、SP、5\|HT水平升高。不同亚型的肠易激综合征患者各指标变化情况不同。     

转基因植物快速检测方法的研究

本试验对转基因植物检测中的DNA提取和PCR扩增程序作了改进。经试验,本研究建立的DNA快速提取法与目前广泛使用的CTAB法相比更为简便,快速和经济,提取的DNA质量主扩增效果无明显差异,可用于多种转基因植物,多种植物组织的DNA提取,利用复合PCR法可在同一反应管中同步检测35N,NOS及CP4－EPSPS基因,明显提高了检测效率。应用本试验建立的DNA快速提取－复合PCR扩增－银染检测技术可在6小时内得出结果,达到了快速,简便,灵敏,可靠的检测目的。     

MiR-223 inhibitor suppresses proliferation and induces apoptosis of thyroid cancer cells by down-regulating aquaporin-1

To investigate the effect of miR-223 on thyroid cancer cells, further to study its potential mechanisms. The difference in miR-223 expression between normal thyroid Nthy-ori3-l cells and thyroid cancer SW579 cells was detected by PCR. The miR-223 overexpression and silencing vector transfection were verified by qRT-PCR. To further investigate the role of miR-223 in AQP-1, the AQP-1 siRNA vector was transfected on the basis of transfection of miR-223 inhibitor vector. The cell proliferation was detected by plate cloning, MTT, and cellular immunofluorescence assays. Cell cycle and apoptosis were detected by flow cytometry. Western blot was used to detect the expression of AQP-1 protein. The expression of miR-223 in SW579 cells was higher than that in normal cells. After transfection with miR-223 mimic, miR-223 expression was increased in SW579 cells. MiR-223 inhibitor transfection can inhibit SW579 cells proliferation, promote apoptosis, and inhibit cell cycle G0/G1 arrest. The SW579 cells proliferation was decreased, and the apoptosis rate was increased after transfection of AQP-1 silencing vector. Compared with the AQP-1 siRNA group, the SW579 cells proliferation rate was further reduced, and the apoptosis rate was significantly increased after co-transfection of miR-223 silencing vector and AQP-1 silencing vector. AQP-1 protein was highly expressed in SW579 cells, and miR-223 inhibitor can down-regulate the expression of APQ-1 protein. The expression AQP-1 protein was significantly reduced after transfected with AQP-1 silencing vector. Inhibition of miR-223 expression could suppress proliferation and promote apoptosis of SW579, and its mechanism is related to down-regulation of APQ-1 protein expression.