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991.
Glucose(Glu) is involved in not only plant physiological and developmental events but also plant responses to abiotic stresses. Here, we found that the exogenous Glu improved root and shoot growth, reduced shoot cadmium(Cd) concentration, and rescued Cdinduced chlorosis in Arabidopsis thaliana(Columbia ecotype,Col-0) under Cd stressed conditions. Glucose increased Cd retained in the roots, thus reducing its translocation from root to shoot signi fi cantly. The most Cd retained in the roots was found in the hemicellulose 1. Glucose combined with Cd(Glu t Cd) treatment did not affect the content of pectin and its binding capacity of Cd while it increased the content of hemicelluloses 1 and the amount of Cd retained in it signi fi cantly. Furthermore, Leadmium Green staining indicated that more Cd was compartmented into vacuoles in Glu t Cd treatment compared with Cd treatment alone, which was in accordance with the R e ssigni fi cant upregulation of the expression of tonoplastlocalized metal transporter genes, suggesting that compartmentation of Cd into vacuoles also contributes to the Glu-alleviated Cd toxicity. Taken together, we demonstrated that Glu-alleviated Cd toxicity is mediated through increasing Cd fi xation in the root cell wall and sequestration into the vacuoles. 相似文献
992.
Yunxia Fang Jiang Hu Jie Xu Haiping Yu Zhenyuan Shi Guosheng Xiong Li Zhu Dali Zeng Guangheng Zhang Zhenyu Gao Guojun Dong Meixian Yan Longbiao Guo Yonghong Wang Qian Qian 《植物学报(英文版)》2015,57(2):151-161
The aerial parts of higher plants are generated from the shoot apical meristem(SAM). In this study, we isolated a small rice(Oryza sativa L.) mutant that showed premature termination of shoot development and was named mini rice 1(mini1). The mutant was first isolated from a japonica cultivar Zhonghua11(ZH11) subjected to ethyl methanesulfonate(EMS)treatment. With bulked segregant analysis(BSA) and map-based cloning method, Mini1 gene was finally fine-mapped to an interval of 48.6 kb on chromosome 9. Sequence analyses revealed a single base substitution from G to A was found in the region, which resulted in an amino acid change from Gly to Asp.The candidate gene Os09g0363900 was predicted to encode a putative adhesion of calyx edges protein ACE(putative HOTHEAD precursor) and genetic complementation experiment confirmed the identity of Mini1. Os09g0363900 contains glucose-methanol-choline(GMC) oxidoreductase and NAD(P)-binding Rossmann-like domain, and exhibits high similarity to Arabidopsis HOTHEAD(HTH). Expression analysis indicated Mini1 was highly expressed in young shoots but lowly in roots and the expression level of most genes involved in auxin biosynthesis and signal transduction were reduced in mutant.We conclude that Mini1 plays an important role in maintaining SAM activity and promoting shoot development in rice. 相似文献
993.
<正>With the effect of global climate change on grain yield and distribution of crops,to understand how plant responds to extreme temperature stress is becoming crucial.Plants have evolved fine-tuned mechanisms in response to a wide variety of environmental stresses.During rice(Oryza sativa L.)domestication,two main genotypes are bred and extendedly 相似文献
994.
高粱、紫苏叶脉密度与光合特性的关系 总被引:2,自引:0,他引:2
叶脉是植物叶片光合作用水分输送的重要结构。为阐述叶脉与光合特性之间的关系,以C4植物高粱(Sorghum bicolor)、C3植物紫苏(Perilla frutescens)为实验材料研究了叶脉密度和光合特性之间的关系。结果表明,与紫苏相比,高粱叶片叶脉密度大,导水能力强,蒸腾速率高,但气孔密度小。进一步分析表明,高粱叶片近轴侧气孔密度占总气孔的比例明显高于紫苏。叶脉密度大的高粱具有较高的净光合速率;而紫苏叶脉密度小,净光合速率也较低。由此表明,较高的叶脉密度有利于支持较高的光合速率,但研究表明叶脉密度和气孔密度可能不存在严格的协同变异关系。研究结果对理解植物光合作用适应有重要意义。 相似文献
995.
996.
Huanwen Wu Liang Zhu Hui Zhang Xiaohua Shi Li Zhang Wenze Wang Huadan Xue Zhiyong Liang 《PloS one》2015,10(2)
BackgroundEpidermal growth factor receptor (EGFR) is highly expressed in pancreatic ductal adenocarcinoma (PDAC) and is involved in tumorigenesis and development. However, EGFR expression alone has limited clinical and prognostic significance. Recently, the cross-talk between EGFR and G-protein-coupled chemokine receptor CXCR4 has become increasingly recognized.MethodsIn the present study, immunohistochemical staining of EGFR and CXCR4 was performed on paraffin-embedded specimens from 131 patients with surgically resected PDAC. Subsequently, the associations between EGFR expression, CXCR4 expression, EGFR/CXCR4 coexpression and clinicopathologic factors were assessed, and survival analyses were performed.ResultsIn total, 64 (48.9%) patients expressed EGFR, 68 (51.9%) expressed CXCR4, and 33 (25.2%) coexpressed EGFR and CXCR4. No significant association between EGFR and CXCR4 expression was observed (P = 0.938). EGFR expression significantly correlated with tumor differentiation (P = 0.031), whereas CXCR4 expression significantly correlated with lymph node metastasis (P = 0.001). EGFR/CXCR4 coexpression was significantly associated with lymph node metastasis (P = 0.026), TNM stage (P = 0.048), and poor tumor differentiation (P = 0.004). By univariate survival analysis, both CXCR4 expression and EGFR/CXCR4 coexpression were significant prognostic factors for poor disease-free survival (DFS) and overall survival (OS). Moreover, EGFR/CXCR4 coexpression significantly increased the hazard ratio for both recurrence and death compared with EGFR or CXCR4 protein expression alone. Multivariate survival analysis demonstrated that EGFR/CXCR4 coexpression was an independent prognostic factor for DFS (HR = 2.33, P<0.001) and OS (HR = 2.48, P = 0.001).ConclusionsIn conclusion, our data indicate that although EGFR expression alone has limited clinical and prognostic significance, EGFR/CXCR4 coexpression identified a subset of PDAC patients with more aggressive tumor characteristics and a significantly worse prognosis. Our results suggest a potentially important "cross-talk" between CXCR4 and EGFR intracellular pathways and indicate that the simultaneous inhibition of these pathways might be an attractive therapeutic strategy for PDAC. 相似文献
997.
We examined whether intermittent administration of parathyroid hormone [1–34] (PTH[1–34]; 60 μg/kg/day) can prevent the negative effects of titanium (Ti) particles on implant fixation and periprosthetic osteolysis in a rat model. Eighteen adult male rats (12 weeks old, bones still growing) received intramedullary Ti implants in their bilateral femurs; 6 rats from the blank group received vehicle injections, and 12 rats from the control group and PTH treatment group received Ti particle injections at the time of operation and intra-articular injections 2 and 4 weeks postoperatively. Six of the rats that received Ti particles from the PTH group also received PTH[1–34] treatment. Six weeks postoperatively, all specimens were collected for assessment by X-ray, micro-CT, biomechanical, scanning electron microscopy (SEM), and dynamic histomorphometry. A lower BMD, BV/TV, Tb.N, maximal fixation strength, and mineral apposition rate were observed in the control group compared to the blank group, demonstrating that a periprosthetic osteolysis model had been successfully established. Administration of PTH[1–34] significantly increased the bone mineral density of the distal femur, BV/TV, Tb.N, Tb.Th, Tb.Sp, Con.D, SMI, and maximal fixation strength in the PTH group compared to that in the control group. SEM revealed higher bone–implant contact, thicker lamellar bone, and larger trabecular bone area in the PTH group than in the control group. A higher mineral apposition rate was observed in the PTH group compared to both the blank and control groups. These findings imply that intermittent administration of PTH[1–34] prevents periprosthetic osteolysis by promoting bone formation. The effects of PTH[1–34] were evaluated at a suprapharmacological dosage to the human equivalent in rats; therefore, additional studies are required to demonstrate its therapeutic potential in periprosthetic osteolysis. 相似文献
998.
Zi Feng Yang Chris Ka Pun Mok Xiao Qing Liu Xiao Bo Li Jian Feng He Wen Da Guan Yong Hao Xu Wei Qi Pan Li Yan Chen Yong Ping Lin Shi Guan Wu Si Hua Pan Ji Cheng Huang Guo Yun Ding Kui Zheng Chang Wen Ke Jin Yan Lin Yong Hui Zhang Horace Hok Yeung Lee Wen Kuan Liu Chun Guang Yang Rong Zhou Joseph Sriyal Malik Peiris Yi Min Li Rong Chang Chen Ling Chen Nan Shan Zhong 《PloS one》2015,10(2)
BackgroundThe second wave of avian influenza H7N9 virus outbreak in humans spread to the Guangdong province of China by August of 2013 and this virus is now endemic in poultry in this region.MethodsFive patients with H7N9 virus infection admitted to our hospital during August 2013 to February 2014 were intensively investigated. Viral load in the respiratory tract was determined by quantitative polymerase chain reaction (Q-PCR) and cytokine levels were measured by bead-based flow cytometery.ResultsFour patients survived and one died. Viral load in different clinical specimens was correlated with cytokine levels in plasma and broncho-alveolar fluid (BALF), therapeutic modalities used and clinical outcome. Intravenous zanamivir appeared to be better than peramivir as salvage therapy in patients who failed to respond to oseltamivir. Higher and more prolonged viral load was found in the sputum or endotracheal aspirates compared to throat swabs. Upregulation of proinflammatory cytokines IP-10, MCP-1, MIG, MIP-1α/β, IL-1β and IL-8 was found in the plasma and BALF samples. The levels of cytokines in the plasma and viral load were correlated with disease severity. Reactivation of herpes simplex virus type 1(HSV-1) was found in three out of five patients (60%).ConclusionExpectorated sputum or endotracheal aspirate specimens are preferable to throat swabs for detecting and monitoring H7N9 virus. Severity of the disease was correlated to the viral load in the respiratory tract as well as the extents of cytokinemia. Reactivation of HSV-1 may contribute to clinical outcome. 相似文献
999.
To evaluate multiple follicular development synchronization after estrogen stimulation in prepubertal mice, follicular responsiveness to gonadotropin superovulation, the prospective reproductive potential and ovarian polycystic ovary syndrome (PCOS)-like symptoms at adulthood, prepubertal mice were intraperitoneally injected with estrogen to establish an animal model with solvent as control. When synchronized tertiary follicles in ovaries, in vitro oocyte maturation and fertilization rates, blastocyst formation rate, developmental potential into offspring by embryo transfer, adult fertility and PCOS-like symptoms, and involved molecular mechanisms were focused, it was found that estrogen stimulation (10μg/gBW) leads to follicular development synchronization at the early tertiary stage in prepubertal mice; reproduction from oocytes to offspring could be realized by means of the artificial reproductive technology though the model mice lost their natural fertility when they were reared to adulthood; and typical symptoms of PCOS, except changes in inflammatory pathways, were not remained up to adulthood. So in conclusion, estrogen can lead to synchronization in follicular development in prepubertal mice, but does not affect reproductive outcome of oocytes, and no typical symptoms of PCOS remained at adulthood despite changes related to inflammation. 相似文献
1000.
Mapping Spatial Variability of Soil Salinity in a Coastal Paddy Field Based on Electromagnetic Sensors 总被引:1,自引:0,他引:1
In coastal China, there is an urgent need to increase land area for agricultural production and urban development, where there is a rapid growing population. One solution is land reclamation from coastal tidelands, but soil salinization is problematic. As such, it is very important to characterize and map the within-field variability of soil salinity in space and time. Conventional methods are often time-consuming, expensive, labor-intensive, and unpractical. Fortunately, proximal sensing has become an important technology in characterizing within-field spatial variability. In this study, we employed the EM38 to study spatial variability of soil salinity in a coastal paddy field. Significant correlation relationship between ECa and EC1:5 (i.e. r >0.9) allowed us to use EM38 data to characterize the spatial variability of soil salinity. Geostatistical methods were used to determine the horizontal spatio-temporal variability of soil salinity over three consecutive years. The study found that the distribution of salinity was heterogeneous and the leaching of salts was more significant in the edges of the study field. By inverting the EM38 data using a Quasi-3D inversion algorithm, the vertical spatio-temporal variability of soil salinity was determined and the leaching of salts over time was easily identified. The methodology of this study can be used as guidance for researchers interested in understanding soil salinity development as well as land managers aiming for effective soil salinity monitoring and management practices. In order to better characterize the variations in soil salinity to a deeper soil profile, the deeper mode of EM38 (i.e., EM38v) as well as other EMI instruments (e.g. DUALEM-421) can be incorporated to conduct Quasi-3D inversions for deeper soil profiles. 相似文献