全文获取类型
收费全文 | 110772篇 |
免费 | 8491篇 |
国内免费 | 6898篇 |
出版年
2024年 | 215篇 |
2023年 | 1451篇 |
2022年 | 3238篇 |
2021年 | 5478篇 |
2020年 | 3581篇 |
2019年 | 4378篇 |
2018年 | 4355篇 |
2017年 | 3237篇 |
2016年 | 4603篇 |
2015年 | 6685篇 |
2014年 | 7879篇 |
2013年 | 8321篇 |
2012年 | 9971篇 |
2011年 | 8878篇 |
2010年 | 5455篇 |
2009年 | 4762篇 |
2008年 | 5598篇 |
2007年 | 4935篇 |
2006年 | 4397篇 |
2005年 | 3342篇 |
2004年 | 2938篇 |
2003年 | 2544篇 |
2002年 | 2209篇 |
2001年 | 2002篇 |
2000年 | 1862篇 |
1999年 | 1844篇 |
1998年 | 1018篇 |
1997年 | 1140篇 |
1996年 | 1018篇 |
1995年 | 922篇 |
1994年 | 942篇 |
1993年 | 668篇 |
1992年 | 993篇 |
1991年 | 838篇 |
1990年 | 613篇 |
1989年 | 560篇 |
1988年 | 488篇 |
1987年 | 411篇 |
1986年 | 389篇 |
1985年 | 390篇 |
1984年 | 212篇 |
1983年 | 198篇 |
1982年 | 137篇 |
1981年 | 114篇 |
1980年 | 107篇 |
1979年 | 115篇 |
1978年 | 78篇 |
1977年 | 60篇 |
1974年 | 74篇 |
1972年 | 62篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
201.
The structure of Z-DNA, currently accepted as a model for all left-handed DNAs, fails to provide convincing explanations for at least four well established properties of left-handed DNA polymers in solution. However, the major discrepancies between theory and experiment are resolved by the structure presently proposed for Z[WC]-DNA, a new left-handed, zig-zag double helix with Watson-Crick-type backbone directions. Structural features of Z[WC]-DNA include the presence of an additional H-bond between each guanine N2-amino group and an adjacent phosphate oxygen, the capacity to form four-stranded, base-matched complexes that should readily precipitate from solution, and backbone progressions that are the same as B-DNA (opposite to Z-DNA). However, since Z[WC]-DNA and Z-DNA have many parameters in common, they could be difficult to distinguish in a majority of existing experiments. In view of the close relationship of the new helix to B-DNA, which allows a relatively unhindered right-to-left transition in handedness, Z[WC]-DNA is theorized to be the left-handed structure preferentially generated in vivo by the torque available in naturally occurring DNA supercoils. 相似文献
202.
A 40 kilodalton rat liver nuclear protein binds specifically to apolipoprotein B mRNA around the RNA editing site. 总被引:8,自引:5,他引:3
下载免费PDF全文
![点击此处可从《Nucleic acids research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Apolipoprotein (apo) B-48 mRNA is the product of RNA editing which consists of a C----U conversion changing a CAA codon encoding Gln-2153 in apoB-100 mRNA to a UAA stop codon in apoB-48 mRNA. In the adult rat, RNA editing occurs both in the small intestine and the liver. We have studied the ability of rat liver nuclear extracts to bind to synthetic apoB mRNA segments spanning the editing site. Using an RNA gel mobility shift assay, we found the sequence-specific binding of a protein(s) to a 65-nucleotide apoB-100 mRNA. UV crosslinking followed by T1 ribonuclease digestion and SDS-polyacrylamide gel electrophoresis demonstrated the formation of a 40 kDa protein-RNA complex when 32P-labeled apoB-100 mRNA was incubated with a rat liver nuclear extract but not with HeLa nuclear extract. Binding was specific for the sense strand of apoB mRNA, and was not demonstrated with single-stranded apoB DNA, or antisense apoB RNA. The complex also failed to form if SDS was present during the UV light exposure. Binding experiments using synthetic apoB mRNAs indicate that the 40 kDa protein would also bind to apoB-48 mRNA but not apoA-I, apoA-IV, apoC-II or apoE mRNA. Experiments using deletion mutants of apoB-100 mRNA indicate efficient binding of wildtype 65-nucleotide (W65), 40-nucleotide (W40) and 26-nucleotide (W26) apoB-100 mRNA segments, but not 10-nucleotide (or smaller) segments of apoB-100 mRNA to the 40 kDa protein. In contrast, two other regions of apoB-100 mRNA, B-5' (bases 1128-3003) and B-3' (bases 11310-11390), failed to bind to the protein. The 40 kDa sequence-specific binding protein in rat liver nuclear extract may play a role in apoB-100 mRNA editing. 相似文献
203.
204.
205.
206.
Assignment of a human cyclin A gene to 4q26-q27 总被引:1,自引:0,他引:1
V Blanquet J A Wang X Chenivesse B Henglein F Garreau C Bréchot C Turleau 《Genomics》1990,8(3):595-597
207.
Summary A high intensity of lectin bindings was demonstrated on the epithelial cells and serosa cells of the regressing right Mullerian ducts (Mds) in the female chick embryos. The strong lectin bindings occurs on, or in the regressing Md cells along with marked surface MIS bindings at the age of day 13. However, at the age of days 5–7 1/2, bindings of lectins were weak. Neither Wheat-germ agglutinin (WGA) or Concanavalin A (Con-A) labelings before MIS-antiserum (MIS-Ab) incubation can block antibody recognitions to the antigens, including MIS and growth hormone at the age of day 13. Our previous studies indicated that after WGA labeling on the surfaces of Md epithelial cells prior to the incubation of MIS-Ab at day 10 did not prevent the recognition of MIS-Ab (Wang 1989). On the contrary, at day 7 1/2, the specific binding of MIS was eliminated after preincubations with lectins and prenatal diethylstilbestrol (DES) treatment at the age of day 5. It is suggested that DES provides a protection to the Mds from MIS-induced regression by preventing the MIS binding to its specific membrane receptors. An increase of extra- and intracellular glycoproteins or carbohydrates of regressing Md epithelial cells were suggested. Internalization of WGA but not MIS molecules was found in Md epithelial cells. The Golgi saccules were negative of lectin bindings. 相似文献
208.
Summary Amino acids, including lysine, glutamic acid, and phenylalanine, in pure solution or in fermentation broth, were extracted with the aqueous two-phase system consisting of polyethylene glycol and salts, giving a very sharp separation. The partition is influenced by the type and the amount of salts used, pH and components of the broth. 相似文献
209.
金线鲃属鱼类一新种(鲤形目:鲤科) 总被引:1,自引:0,他引:1
作者于1986年6月在贵州盘县一溶洞的水体中采到金线鲃属鱼类一新种,命名为角金线鲃,新种Sinocyclocheilus angularis sp. nov.。本新种形状怪异,头背缘末端具一朝前的角状突,其内有顶骨突;眼正常;侧线鳞明显;鳔后室发达等特征而易与金线鲃属其他种相区别。 相似文献
210.
本文研究了温度、盐度和重金属等几种因子对缢蛏和长牡蛎幼体趋地性和趋光性行为的影响。结果表明:1)降低温度能增强缢蛏幼体的正趋地性,而长牡蛎幼体却由正趋地性转变为负趋地性,同时两种幼体的正趋光性受到抑制作用;升高温度对缢蛏幼体趋光性的影响不明显,但能加强牡蛎幼体的正趋光性。2)盐度提高时缢蛏幼体的正趋光性趋于增强,正趋地性趋于减弱。3)重金属Cu和Zn在各自的亚致死浓度下具有提高缢蛏幼体正趋地性和降低正趋光性的作用,而牡蛎幼体的正趋地性却趋于减弱,正趋光性趋于增强。本文据上述幼体的行为变化,还讨论了这几种因子对幼体垂直分布的调节作用。 相似文献