Acrylonitrile-induced sister-chromatid exchanges and DNA single-strand breaks in adult human bronchial epithelial cells

The ability of acrylonitrile to induce cytotoxicity, sister-chromatid exchanges and DNA single-strand breaks was studied in cultured human bronchial epithelial cells. The toxic effect as determined by cloning efficiency was observed at a dose of 600 micrograms/ml but not at doses of both 150 and 300 micrograms/ml. The frequency of sister-chromatid exchange in untreated cells was 3.7 +/- 1.3 per cell. In contrast, cells treated with acrylonitrile at 150 and 300 micrograms/ml exhibited 6.6 +/- 1.3 and 10.7 +/- 1.7 sister-chromatid exchanges per metaphase, respectively. DNA single-strand breaks were induced by acrylonitrile at dose levels of 200 and 500 micrograms/ml. The genotoxic effects on human bronchial epithelial cells that were directly exposed to acrylonitrile are of interest in relation to evidence for the higher lung cancer incidence of acrylonitrile workers in epidemiological studies.     

Cytogenetic identification of interspecies cell-line contamination: procedures for non-cytogeneticists

A few simple procedures and cytological characteristics are described for identification of cell-line contamination involving human cells, or cells of several species of common laboratory and domestic animals. These include gross chromosome morphology, C-banding, fluorescence, and the nucleolus organizer regions as revealed by silver straining.     

The effect of sodium selenite toxicity on tissue distribution of zinc,iron, and copper in rats

Male Sprague-Dawley rats were used to determine the effects of suptoxic and toxic concentrations of selenite in the drinking water on tissue distribution of zinc (Zn), iron (Fe), and copper (Cu). Se (as sodium selenite) was provided in drinking water at concentrations of 0, 2, 4, and 8 ppm. At 19 d, half of the rats in 4 and 8 ppm Sesupplemented groups were kept on drinking water alone for additional 13 d. All rats were sacrificed at the end of 32 d of experiment. Heart, liver, and kidney were analyzed for the concentrations of Fe, Zn, and Cu by atomic absorption spectrophotometry and of Se by a fluorometric method. Results indicated that rats receiving 4 and 8 ppm Se in drinking water showed a marked reduction in food intake and a reduced growth rate. These adverse effects were quickly reversed when high Se intake was discontinued. Se toxicity caused minimal change in zinc status, reduced tissue iron concentrations and caused a marked increase in copper contents in heart, liver, and kidney. The latter findings were only partly reversed after removal of Se in drinking water. The accumulation of Cu in the tissues of Se-toxic rats provides the evidence of some interaction between Se and Cu.     

植物分类学中的繁殖生物学问题

繁殖生物学的知识有助于对分类学上复杂性问题的理解,从而大大地有利于我们作出明智的分类估价,但它不一定为我们提供解决问题的钥匙。异体受精增加群体的杂合性和变异性。互交繁育的程度限定了表型变异的式样;互交繁育受阻往往造成分类群表型上的间断。异形不亲和性系统(如花柱异长、雌雄异株等等)给分类学家提供可鉴别的形态差异来划分密切近缘的分类群,但也有由此而造成分类实践上混乱的情况。自体受精增加群体的纯合性;相继世代持续的自体受精使群体变成纯系或同形小种。但对自体受精植物的分类处理并不存在既定的原则,而现在大多数学者不支持把每一个纯系或小种(或约当种)都定为种的做法仅仅反映了当前分类学的时流。但普遍认为,对自体受精类群的分类处理应不同于异体受精类群。无融合生殖是自体受精的一种极端的形式,包括营养生殖和无融合结籽(包括不定胚生殖、无孢子生殖、双倍孢子生殖、假受精)两大类。在任何情况下,胚总是完全由母体组织形成,因此后代在遗传上与它的母体完全相同。由于许多无融合类群都是兼性的,即既能产生无性的种子,又能产生受精的种子,而且又常与杂交和多倍化密切相关,因此具有特别复杂的变异式样。这三套过程同时存在的类群,称为无融合种综,分类学上称为“困难属”,种的划     

小果青钱柳的分类地位

<正> 钟补求教授于1936年根据采自安徽黄山的模式标本而发表小果青钱柳Pterocarya mi-cropaliurus Tsoong新种时指出,该种与青钱柳的主要区别在于:(1)果实(包括果翅)较小,直径不超过2.5匣米,而在青钱柳则为3—7匣米;(2)果序较短,长约为青钱柳之半;(3)小叶数目较多,通常9—11枚,且较青钱柳为狭小;(4)小叶的锯齿较密较尖,最大的小叶通常每边有60个以上的锯齿,而在青钱柳则通常不到40个。但根据《中国植物志》第二十一卷(以下简称《植物志》),小果青钱柳应为青钱柳Cyclocarya paliurus(Batal.)Iljinsk.的异名。该《植物志》写道:“本种的小叶大小及数目、果翅的形状及大小变异极大,某些极端类型看来好象可以划分成各别的种,但都存在有中间类型,并且也无一定的地理分布限定,因此它必然是一个自然的种”。为此,我们对有关的腊叶材料进行了     

抗胶质瘤细胞单克隆抗体SZ-38及其识别抗原的生化特征

本文以人脑胶质瘤细胞系SHG-44为抗原,利用杂交瘤技术建立了一株恒定地分泌抗胶质瘤细胞单克隆抗体(McAb)的杂交瘤细胞株SZ-38。McAb SZ-38与9/10胶质瘤细胞系发生强结合反应。而与淋巴细胞、ABO型红细胞等所有正常血液细胞及绝大多数被检测肿瘤细胞系无反应。经免疫转移电泳及免疫沉淀法鉴定,该McAb识别抗原为胶质瘤细胞膜上Mw47,000糖蛋白。应用SPA-Sepharose 4B提纯MeAb SZ-38,再把纯化抗体交联于Sepharose 4B,以McAb亲和层析法提取SZ-38抗原,经SDS-PAGE证实其有较高的纯度。     

The lipid composition and conversion of tryptamine toN b-acetyltryptamine in aCatharanthus roseus cell line without indole alkaloids

Summary A cell line, NA13-2, was selected as a rapidly growing colony of protoplasts from a UV(254 nm)-fluorescent cell line, NA13-1, which originated from a tryptamine-resistant strain ofCatharanthus roseus NA13. Cell line NA13-2 lost the capability to produce indole alkaloids. Tryptophan fed to these cells was converted toN _b-acetyltryptamine as the major product. The free acetyl coenzyme A content of NA13-2 cells was 50% higher than in the mother cells. The total lipid content of the NA13-2 cells was 2.5-fold that in the NA13 cells. In spite of the similarity in the fatty acid content to that of the mother cell line NA13, the total lipid extract of NA13-2 cells appeared as a wax instead of an oil, resulting from the presence of sterol esters.This paper was presented in part at the Annual Meeting of the Society for Industrial Microbiology, Boston, MA, 1985, and the International Congress of the Plant Tissue Culture Association, Minneapolis, MN, 1986.     

HIV-1 protease inhibitory activity of L-694,746, a novel metabolite of L-689,502.

L-689,502 is a potent inhibitor of HIV-1 protease activity in vitro. Microbial biotransformations of L-689,502 by cultures belonging to the genus Streptomyces sp. were performed. Extracts of culture broths were examined for the production of metabolites of L-689,502 that could inhibit HIV-1 protease activity. One culture, MA 6804 (Streptomyces lavendulae, ATCC 55095), produced L-694,746 that, while being structurally related to L-689,502, is a novel metabolite and a potent inhibitor of HIV-1 protease.