全文获取类型
收费全文 | 44894篇 |
免费 | 3983篇 |
国内免费 | 5906篇 |
专业分类
54783篇 |
出版年
2024年 | 145篇 |
2023年 | 636篇 |
2022年 | 1476篇 |
2021年 | 2380篇 |
2020年 | 1689篇 |
2019年 | 2134篇 |
2018年 | 2009篇 |
2017年 | 1550篇 |
2016年 | 2053篇 |
2015年 | 2967篇 |
2014年 | 3527篇 |
2013年 | 3704篇 |
2012年 | 4492篇 |
2011年 | 4052篇 |
2010年 | 2542篇 |
2009年 | 2251篇 |
2008年 | 2629篇 |
2007年 | 2320篇 |
2006年 | 2032篇 |
2005年 | 1672篇 |
2004年 | 1361篇 |
2003年 | 1266篇 |
2002年 | 1044篇 |
2001年 | 714篇 |
2000年 | 618篇 |
1999年 | 580篇 |
1998年 | 374篇 |
1997年 | 350篇 |
1996年 | 319篇 |
1995年 | 256篇 |
1994年 | 260篇 |
1993年 | 183篇 |
1992年 | 186篇 |
1991年 | 177篇 |
1990年 | 135篇 |
1989年 | 118篇 |
1988年 | 93篇 |
1987年 | 57篇 |
1986年 | 72篇 |
1985年 | 78篇 |
1984年 | 40篇 |
1983年 | 48篇 |
1982年 | 37篇 |
1981年 | 24篇 |
1980年 | 13篇 |
1979年 | 22篇 |
1978年 | 11篇 |
1974年 | 8篇 |
1973年 | 13篇 |
1971年 | 11篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
91.
Wang-Shan Zheng Yao-Xi He Chao-Ying Cui Ouzhuluobu Dejiquzong Yi Peng Cai-Juan Bai Duojizhuoma Gonggalanzi Bianba Baimakangzhuo Yong-Yue Pan Qula Kangmin Cirenyangji Baimayangji Wei Guo Yangla Hui Zhang Xiao-Ming Zhang Yong-Bo Guo Shu-Hua Xu Hua Chen Sheng-Guo Zhao Yuan Cai Shi-Ming Liu Tian-Yi Wu Xue-Bin Qi Bing Su 《动物学研究》2017,38(3)
The genetic adaptation of Tibetans to high altitude hypoxia likely involves a group of genes in the hypoxic pathway,as suggested by earlier studies.To test the adaptive role of the previously reported candidate gene EP300 (histone acetyltransferase p300),we conducted resequencing of a 108.9 kb gene region of EP300 in 80 unrelated Tibetans.The allele-frequency and haplotype-based neutrality tests detected signals of positive Darwinian selection on EP300 in Tibetans,with a group of variants showing allelic divergence between Tibetans and lowland reference populations,including Han Chinese,Europeans,and Africans.Functional prediction suggested the involvement of multiple EP300 variants in gene expression regulation.More importantly,genetic association tests in 226 Tibetans indicated significant correlation of the adaptive EP300 variants with blood nitric oxide (NO) concentration.Collectively,we propose that EP300 harbors adaptive variants in Tibetans,which might contribute to high-altitude adaptation through regulating NO production. 相似文献
92.
Biologic drugs, such as monoclonal antibodies, are commonly made using mammalian cells in culture. The cell lines used for manufacturing should ideally be clonal, meaning derived from a single cell, which represents a technically challenging process. Fetal bovine serum is often used to support low cell density cultures, however, from a regulatory perspective, it is preferable to avoid animal‐derived components to increase process consistency and reduce the risk of contamination from adventitious agents. Chinese hamster ovary (CHO) cells are the most widely used cell line in industry and a large number of serum‐free, protein‐free, and fully chemically defined growth media are commercially available, although these media alone do not readily support efficient single cell cloning. In this work, we have developed a simple, fully defined, single‐cell cloning media, specifically for CHO cells, using commercially available reagents. Our results show that a 1:1 mixture of CD‐CHO? and DMEM/F12 supplemented with 1.5 g/L of recombinant albumin (Albucult®) supports single cell cloning. This formulation can support recovery of single cells in 43% of cultures compared to 62% in the presence of serum. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012 相似文献
93.
94.
95.
96.
Roads are a prominent feature of many landscapes, and high road densities create correspondingly high ecological impacts by altering landscape patterns, interrupting ecological flows, increasing erosion, fragmenting habitat, and facilitating the spread of invasive species. Here, we describe the construction of “near-natural greenways” that produce environmentally and socially harmonious road systems that meet the needs of both the environment and the socioeconomic development in China and that satisfy environmental, ecological, educational, traffic safety, economic, and sustainable developmental goals. In the last decade, China has embarked upon the implementation of a network of near-natural greenways with the vision of linking economic development with nature conservation by means of improved rehabilitation of existing road systems and improved construction of new roads. Ecological science, and especially landscape ecology, will play an important role in the planning and implementation of future near-natural greenways in China and around the world. 相似文献
97.
98.
99.
100.
Wavefront shaping can compensate the wavefront distortions in deep tissue focusing, leading to an improved penetration depth. However, when using the backscattered signals as the feedback, unexpected compensation bias may be introduced, resulting in focusing position deviations or even no focus in the illumination focal plane. Here we investigated the reliability of wavefront shaping based on coherent optical adaptive technique in deep tissue focusing by measuring the position deviations between the foci in the illumination focal plane and the epi‐detection plane. The experimental results show that when the penetration depth reaches 150 μm in mouse brain tissue (with scattering coefficient ~22.42 mm?1) using a 488 nm laser and an objective lens with 0.75 numerical aperture, the center of the real focus will deviate out of one radius range of the Airy disk while the optimized focus in the epi‐detection plane maintained basically at the center. With the penetration depth increases, the peak to background ratio of the focus in the illumination focal plane decreases faster than that in the epi‐detection plane. The results indicate that when the penetration depth reaches 150 μm, feedback based on backscattered signals will make wavefront shaping lose its reliability, which may provide a guidance for applications of non‐invasive precise optogenetics or deep tissue optical stimulation using wavefront shaping methods. A, Intensity distribution in the epi‐detection plane and the illumination focal plane before and after correction, corresponding to brain sections with 250 and 300 μm thickness, respectively. Scale bar is 2 μm. B, Averaged focusing deviations in the epi‐detection plane (optimized) and the illumination focal plane (monitored) after compensation. The unit of the ordinate is one Airy disk diameter. Black dashed line represents one Airy disk radius. Bars represent the SE of each measurement set. 相似文献