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991.
992.
Zhong Q Zhang Q Wang Z Qi J Chen Y Li S Sun Y Li C Lan X 《Marine biotechnology (New York, N.Y.)》2008,10(3):310-318
Differential expression of genes is crucial to embryogenesis. The analysis of gene expression requires appropriate references
that should be minimally regulated during the embryonic development. To select the most stable genes for gene normalization,
the expression profiles of eight commonly used reference genes (ACTB, GAPDH, rpL17, α-Tub, EF1-α, UbcE, B2M, and 18S rRNA)
were examined during Japanese flounder (Paralichthys olivaceus) embryonic development using quantitative real-time polymerase chain reaction. It was found that all seven mRNA genes appeared
to be developmentally regulated and exhibited significant variation of expression. However, further analyses revealed the
stage-specific expression stability. Hence when normalization using these mRNA genes, the differential and stage-related expression
should be considered. 18S rRNA gene, on the other hand, showed the most stable expression and could be recommended as a suitable
reference gene during all embryonic developmental stages in P. olivaceus. In summary, our results provided not only the appropriate reference gene for embryonic development research in P. olivaceus, but also possible guidance to reference gene selection for embryonic gene expression analyses in other fish species. 相似文献
993.
Wenying Shou Ramzi Azzam Susan L Chen Michael J Huddleston Christopher Baskerville Harry Charbonneau Roland S Annan Steve A Carr Raymond J Deshaies 《BMC molecular biology》2002,3(1):3-14
Background
In S. cerevisiae, the mitotic exit network (MEN) proteins, including the Polo-like protein kinase Cdc5 and the protein phosphatase Cdc14, are required for exit from mitosis. In pre-anaphase cells, Cdc14 is sequestered to the nucleolus by Net1 as a part of the RENT complex. When cells are primed to exit mitosis, the RENT complex is disassembled and Cdc14 is released from the nucleolus. 相似文献994.
S S Chen J W Bohn F T Liu D H Katz 《Journal of immunology (Baltimore, Md. : 1950)》1981,127(1):166-173
995.
Gradients of disturbance and environmental conditions shape coral community structure for south‐eastern Indian Ocean reefs 下载免费PDF全文
Jens Zinke James P. Gilmour Rebecca Fisher Marji Puotinen Joseph Maina Emily Darling Michael Stat Zoe T. Richards Timothy R. McClanahan Maria Beger Cordelia Moore Nicholas A. J. Graham Ming Feng Jean‐Paul A. Hobbs Scott N. Evans Stuart Field George Shedrawi Russ C. Babcock Shaun K. Wilson 《Diversity & distributions》2018,24(5):605-620
996.
997.
Jui-Hung Tai Hsiao-Yu Sun Yi-Cheng Tseng Guanghao Li Sui-Yuan Chang Shiou-Hwei Yeh Pei-Jer Chen Shu-Miaw Chaw Hurng-Yi Wang 《Molecular biology and evolution》2022,39(9)
One of the unique features of SARS-CoV-2 is its apparent neutral evolution during the early pandemic (before February 2020). This contrasts with the preceding SARS-CoV epidemics, where viruses evolved adaptively. SARS-CoV-2 may exhibit a unique or adaptive feature which deviates from other coronaviruses. Alternatively, the virus may have been cryptically circulating in humans for a sufficient time to have acquired adaptive changes before the onset of the current pandemic. To test the scenarios above, we analyzed the SARS-CoV-2 sequences from minks (Neovision vision) and parental humans. In the early phase of the mink epidemic (April to May 2020), nonsynonymous to synonymous mutation ratio per site in the spike protein is 2.93, indicating a selection process favoring adaptive amino acid changes. Mutations in the spike protein were concentrated within its receptor-binding domain and receptor-binding motif. An excess of high-frequency derived variants produced by genetic hitchhiking was found during the middle (June to July 2020) and late phase I (August to September 2020) of the mink epidemic. In contrast, the site frequency spectra of early SARS-CoV-2 in humans only show an excess of low-frequency mutations, consistent with the recent outbreak of the virus. Strong positive selection in the mink SARS-CoV-2 implies that the virus may not be preadapted to a wide range of hosts and illustrates how a virus evolves to establish a continuous infection in a new host. Therefore, the lack of positive selection signal during the early pandemic in humans deserves further investigation. 相似文献
998.
999.
1000.
Space exploration by dendritic cells requires maintenance of myosin II activity by IP3 receptor 1 下载免费PDF全文
Paola Solanes Marine Bretou Franziska Lautenschlaeger Paolo Maiuri Emmanuel Terriac Maria‐Isabel Thoulouze Pierre Launay Matthieu Piel Pablo Vargas Ana‐Maria Lennon‐Duménil 《The EMBO journal》2015,34(6):798-810
Dendritic cells (DCs) patrol the interstitial space of peripheral tissues. The mechanisms that regulate their migration in such constrained environment remain unknown. We here investigated the role of calcium in immature DCs migrating in confinement. We found that they displayed calcium oscillations that were independent of extracellular calcium and more frequently observed in DCs undergoing strong speed fluctuations. In these cells, calcium spikes were associated with fast motility phases. IP3 receptors (IP3Rs) channels, which allow calcium release from the endoplasmic reticulum, were identified as required for immature DCs to migrate at fast speed. The IP3R1 isoform was further shown to specifically regulate the locomotion persistence of immature DCs, that is, their capacity to maintain directional migration. This function of IP3R1 results from its ability to control the phosphorylation levels of myosin II regulatory light chain (MLC) and the back/front polarization of the motor protein. We propose that by upholding myosin II activity, constitutive calcium release from the ER through IP3R1 maintains DC polarity during migration in confinement, facilitating the exploration of their environment. 相似文献