Severe Metabolic Acidemia in Infants: Clinical and Therapeutic Aspects

A study of 10 infants in severe metabolic acidemia (pH below 7) led to the conclusion that the clinical signs—hyperventilation, coma or lethargy, peripheral vascular collapse, a significantly palpable liver, and abdominal distension—may all be directly related to the metabolic acidemia. In five of 10 infants, an initial erroneous diagnosis of congestive heart failure or pneumonia was made. Dramatic clinical improvement followed correction of the acidemia with rapid intravenous administration of sodium bicarbonate. This rapid administration of sodium bicarbonate was safe, provided hypocalcemia was recognized and treated in its early stages. In severe metabolic acidemia the measurement of blood bicarbonate alone does not reflect adequately the magnitude of the acid-base derangement and repeated measurements of hydrogen ion concentration, Pco₂ and bicarbonates are needed to evaluate and treat such infants correctly.     

Fluoreszenzmarkierung von Tumor- und Leber-DNS der Ratte

Zusammenfassung Es wird eine Methode zur Fluoreszenzmarkierung von DNS, die aus Guérin-Rattentumoren und Rattenlebern isoliert wurde, mitgeteilt. Zur Kopplung wurde 1-Dimethylamino-naphthalin-sulfochlorid-5 (DIS) verwendet. Die Ratten intravenös applizierte markierte DNS stellt sich im Gewebsschnitt durch ihre gelbe bzw. intensiv gelbgrüne Fluoreszenz dar.

---

Summary This paper informs of a method of fluorescence marking of DNA isolated from Guérin rat tumors and rat livers. For coupling we used 1-dimethylaminonaphthalene-sulphochloride-5 (DIS). The marked DNA injected intravenously into rats presented itself by yellow or intense yellowish green fluorescence.

---

---

Zum hundertjährigen Bestehen des Lehrstuhls für Pathologie der Universität Rostock.     

The inheritance of some characters in the mung bean,Phaseolus aureus roxb

Four allelic pairs were found to segregate from the cross between two mung bean varieties:rrGGSSdd×RRggssDD. R.rr stands for red versus green cotyledons, hypocotyl and top of leaflet stalk, and for beige yellow versus bright yellow flowers. Seed coat differences areG.-gg for green versus yellow (or brownish) basic colour,S.-ss for spotted (mottled) versus non-spotted (self colour), andD.-dd for dull versus shiny surface.In the spotted class (S.), R.-rr conditions spot covered (SS) and dark spotted (Ss) versus light spotted (SS andSs). Consequently, the F₁ is dark spotted.The recombination value betweenR-r andD-d was 15%.The findings are compared with data from litterature onPhaseolus aureus and its close relatives.