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951.
In the renal medullary thick ascending limb (MTAL), inhibiting the basolateral NHE1 Na(+)/H(+) exchanger with amiloride or nerve growth factor (NGF) results secondarily in inhibition of the apical NHE3 Na(+)/H(+) exchanger, thereby decreasing transepithelial HCO3- absorption. MTALs from rats were studied by in vitro microperfusion to identify the mechanism underlying cross-talk between the two exchangers. The basolateral addition of 10 microM amiloride or 0.7 nM NGF decreased HCO3- absorption by 27-32%. Jasplakinolide, which stabilizes F-actin, or latrunculin B, which disrupts F-actin, decreased basal HCO3- absorption by 30% and prevented the inhibition by amiloride or NGF. Jasplakinolide had no effect on HCO3- absorption in tubules bathed with amiloride or a Na(+)-free bath to inhibit NHE1. Jasplakinolide and latrunculin B did not prevent inhibition of HCO3- absorption by vasopressin or stimulation by hyposmolality, factors that regulate HCO3- absorption through primary effects on apical Na(+)/H(+) exchange. Treatment of MTALs with amiloride or NGF for 15 min decreased polymerized actin with no change in total cell actin, as assessed both by fluorescence microscopy and by actin Triton X-100 solubility. Jasplakinolide prevented amiloride-induced actin remodeling. Vasopressin, which inhibits HCO3- absorption by an amount similar to that observed with amiloride and NGF but does not act via NHE1, did not affect cellular F-actin content. These results indicate that basolateral NHE1 regulates apical NHE3 and HCO3- absorption in the MTAL by controlling the organization of the actin cytoskeleton. 相似文献
952.
Marshall L. Deline Tracy L. Vrablik Jennifer L. Watts 《Journal of visualized experiments : JoVE》2013,(81)
Fatty acids are essential for numerous cellular functions. They serve as efficient energy storage molecules, make up the hydrophobic core of membranes, and participate in various signaling pathways. Caenorhabditis elegans synthesizes all of the enzymes necessary to produce a range of omega-6 and omega-3 fatty acids. This, combined with the simple anatomy and range of available genetic tools, make it an attractive model to study fatty acid function. In order to investigate the genetic pathways that mediate the physiological effects of dietary fatty acids, we have developed a method to supplement the C. elegans diet with unsaturated fatty acids. Supplementation is an effective means to alter the fatty acid composition of worms and can also be used to rescue defects in fatty acid-deficient mutants. Our method uses nematode growth medium agar (NGM) supplemented with fatty acidsodium salts. The fatty acids in the supplemented plates become incorporated into the membranes of the bacterial food source, which is then taken up by the C. elegans that feed on the supplemented bacteria. We also describe a gas chromatography protocol to monitor the changes in fatty acid composition that occur in supplemented worms. This is an efficient way to supplement the diets of both large and small populations of C. elegans, allowing for a range of applications for this method. 相似文献
953.
Anjali Teckchandani Natalie Toida Jake Goodchild Christine Henderson Julian Watts Bernd Wollscheid Jonathan A. Cooper 《The Journal of cell biology》2009,186(1):99-111
Clathrin-associated endocytic adapters recruit cargoes to coated pits as a first step in endocytosis. We developed an unbiased quantitative proteomics approach to identify and quantify glycoprotein cargoes for an endocytic adapter, Dab2. Surface levels of integrins β1, α1, α2, and α3 but not α5 or αv chains were specifically increased on Dab2-deficient HeLa cells. Dab2 colocalizes with integrin β1 in coated pits that are dispersed over the cell surface, suggesting that it regulates bulk endocytosis of inactive integrins. Depletion of Dab2 inhibits cell migration and polarized movement of integrin β1 and vinculin to the leading edge. By manipulating intracellular and surface integrin β1 levels, we show that migration speed correlates with the intracellular integrin pool but not the surface level. Together, these results suggest that Dab2 internalizes integrins freely diffusing on the cell surface and that Dab2 regulates migration, perhaps by maintaining an internal pool of integrins that can be recycled to create new adhesions at the leading edge. 相似文献
954.
Bryan D. Watts Fletcher M. Smith Chance Hines Laura Duval Diana J. Hamilton Tim Keyes Julie Paquet Lisa Pirie‐Dominix Jennie Rausch Barry Truitt Brad Winn Paul Woodard 《Journal of avian biology》2021,52(1)
Migrant shorebirds operate within a series of landscapes and must adjust their daily activities to achieve seasonal time and energy objectives. Night roosts are essential landscape elements that provide safety from predators for many shorebird species. What costs migrants incur to use night roosts and how these costs vary across staging sites are poorly understood. We tracked 42 adult whimbrels Numenius phaeopus with satellite transmitters and used night locations to delineate 39 night roosts during spring and fall migration. We used daytime locations to measure round‐trip commuting distances between night roosts and foraging areas and estimated daily commuting costs including distance, time and metabolic energy expenditure. We identified night roosts on offshore islands (n = 20) and onshore locations including along habitat edges (n = 13) and on topographic highs within extensive marshes (n = 6). Mean daily commuting costs varied between roosts. Whimbrels took 3.9–52.1 min (median = 15.2) to fly 3.1–42.2 km (median = 12.3) which costs 6.1–82.4 kj (median = 22.3) in lean mass energy expenditure and 8.1–109.2 kj (median = 31.5) in leaving mass energy. Birds using offshore roosts had twice the commuting distance and associated costs compared to those using onshore roosts. The contribution of commuting costs to the premigratory energy budget ranged from 1.5 to 18.8% with costs for nearly 30% of roosts exceeding 10%. Commuting costs to and from night roosts appear to be biologically relevant within some staging sites and should be considered among other constraints faced by migrants during stopover periods when food or time is limiting. 相似文献
955.
The C. elegans par-4 gene encodes a putative serine-threonine kinase required for establishing embryonic asymmetry 总被引:3,自引:0,他引:3
During the first cell cycle of Caenorhabditis elegans embryogenesis, asymmetries are established that are essential for determining the subsequent developmental fates of the daughter cells. The maternally expressed par genes are required for establishing this polarity. The products of several of the par genes have been found to be themselves asymmetrically distributed in the first cell cycle. We have identified the par-4 gene of C. elegans, and find that it encodes a putative serine-threonine kinase with similarity to a human kinase associated with Peutz-Jeghers Syndrome, LKB1 (STK11), and a Xenopus egg and embryo kinase, XEEK1. Several strong par-4 mutant alleles are missense mutations that alter conserved residues within the kinase domain, suggesting that kinase activity is essential for PAR-4 function. We find that the PAR-4 protein is present in the gonads, oocytes and early embryos of C. elegans, and is both cytoplasmically and cortically distributed. The cortical distribution begins at the late 1-cell stage, is more pronounced at the 2- and 4-cell stages and is reduced at late stages of embryonic development. We find no asymmetry in the distribution of PAR-4 protein in C. elegans embryos. The distribution of PAR-4 protein in early embryos is unaffected by mutations in the other par genes. 相似文献
956.
Pauly TA Ekstrom JL Beebe DA Chrunyk B Cunningham D Griffor M Kamath A Lee SE Madura R Mcguire D Subashi T Wasilko D Watts P Mylari BL Oates PJ Adams PD Rath VL 《Structure (London, England : 1993)》2003,11(9):1071-1085
Sorbitol dehydrogenase (hSDH) and aldose reductase form the polyol pathway that interconverts glucose and fructose. Redox changes from overproduction of the coenzyme NADH by SDH may play a role in diabetes-induced dysfunction in sensitive tissues, making SDH a therapeutic target for diabetic complications. We have purified and determined the crystal structures of human SDH alone, SDH with NAD(+), and SDH with NADH and an inhibitor that is competitive with fructose. hSDH is a tetramer of identical, catalytically active subunits. In the apo and NAD(+) complex, the catalytic zinc is coordinated by His69, Cys44, Glu70, and a water molecule. The inhibitor coordinates the zinc through an oxygen and a nitrogen atom with the concomitant dissociation of Glu70. The inhibitor forms hydrophobic interactions to NADH and likely sterically occludes substrate binding. The structure of the inhibitor complex provides a framework for developing more potent inhibitors of hSDH. 相似文献
957.
958.
David P. Watts 《American journal of primatology》1988,15(3):195-211
Data on the time budgets of mountain gorillas (Gorilla gorilla beringei) were collected during field studies in the Virunga Volcanoes region of Rwanda and Zaire. Focal sampling was used to determine the proportion of time that individuals of different age/sex classes spent in several mutually exclusive activity states. The gorillas spent the majority of daylight hours feeding; most of the rest of the day was devoted to resting, with little time spent moving or engaged in social activity. The time budget varied among the different subhabitats used by the gorillas, and the gorillas satisfied subsistence needs more quickly when in areas where food was more abundant and/or of better nutritional quality. Silverbacks spent more time feeding than all other age/sex classes, but age/sex class differences were not great. All age/sex classes responded to variability in habitat quality in similar fashion. Unlike the case for many other primates, there was no significant seasonal variation in time budgets. There was a direct relationship between group size and time spent feeding, although variation in relation to group size was lower than that in relation to variation in habitat quality. These results are consistent with the relationship of feeding time to body size in primates. They are also consistent, with other evidence that social foraging entails a cost to gorilla females, but that this cost is low in comparison to those faced by many other primates. Permanent association with males apparently offers little ecological disadvantage to females, who are likely to be more than compensated by mutualistic benefits. 相似文献
959.
Molecular weights of all hemocyanin aggregates which can be homogeneously isolated have been measured by sedimentation equilibrium. The larger aggregates, which are the ones present under physiological conditions, are, to a very close approximation, integral multiples of a 4.4 x 10(6)-dalton, 60 S species. Dissociation of the 60 S species at high pH gives heterogeneous samples in which the smallest species has a molecular weight of 300,000. The smallest subunit which can be produced in denaturing solvents also has a molecular weight of 300,000. 相似文献
960.
Charles W. Purdy Russell H. Raleigh James K. Collins Jeffery L. Watts David C. Straus 《Current microbiology》1997,34(4):244-249
Ninety-one isolates of Pasteurella multocida (Pm)
and 124 of Pasteurella haemolytica (Ph) were recovered from the lungs
of calves that died of bovine respiratory tract disease (BRTD). Nine Pm
enzyme profiles (A through I) and 9 Ph enzyme profiles (J through R) were
determined for the Pasteurella isolates. The Pm isolates were
relatively evenly divided among the enzyme profiles, with one exception,
profile I. The Ph isolates were not evenly distributed among the profiles.
Fifty of the 91 Pm isolates were serotyped. Forty-two Pm isolates were
positive for capsule type A, and 8 were untypable. Five somatic type antigen
profiles (3; 3,4; 3,7; 3,4,7; and 4) were identified among the 50 serotyped
Pm isolates; one isolate was untypable. The Ph isolates were further divided
through serotyping and grouped as follows: 74 (60%) Pasteurella
haemolytica A1 (PhA1), 12 (10%) PhA2, 4 (3%) PhA5, and 34
(27%) PhA6. Eighty-one percent of the Ph serotypes were clustered in
the M and N enzyme profile. The P enzyme profile was almost unique to PhA2 (8
of 12, 67% of PhA2 isolates). Results of this study indicate a need to
collect more data on Ph serotypes at the state veterinary diagnostic
laboratories.
Received: 29 August 1996 / Accepted: 15 October 1996 相似文献