全文获取类型
收费全文 | 4189篇 |
免费 | 400篇 |
专业分类
4589篇 |
出版年
2021年 | 42篇 |
2020年 | 33篇 |
2018年 | 38篇 |
2016年 | 45篇 |
2015年 | 68篇 |
2014年 | 103篇 |
2013年 | 136篇 |
2012年 | 162篇 |
2011年 | 142篇 |
2010年 | 95篇 |
2009年 | 82篇 |
2008年 | 132篇 |
2007年 | 139篇 |
2006年 | 126篇 |
2005年 | 146篇 |
2004年 | 153篇 |
2003年 | 117篇 |
2002年 | 106篇 |
2001年 | 119篇 |
2000年 | 119篇 |
1999年 | 108篇 |
1998年 | 51篇 |
1997年 | 55篇 |
1996年 | 56篇 |
1995年 | 39篇 |
1994年 | 54篇 |
1993年 | 61篇 |
1992年 | 112篇 |
1991年 | 95篇 |
1990年 | 82篇 |
1989年 | 84篇 |
1988年 | 76篇 |
1987年 | 70篇 |
1986年 | 74篇 |
1985年 | 82篇 |
1984年 | 70篇 |
1983年 | 47篇 |
1982年 | 54篇 |
1981年 | 37篇 |
1980年 | 53篇 |
1979年 | 67篇 |
1978年 | 56篇 |
1977年 | 58篇 |
1976年 | 55篇 |
1975年 | 45篇 |
1974年 | 37篇 |
1973年 | 45篇 |
1972年 | 33篇 |
1969年 | 31篇 |
1966年 | 34篇 |
排序方式: 共有4589条查询结果,搜索用时 0 毫秒
11.
12.
Q Yuan W M Jiang D Hollander E Leung J D Watson G W Krissansen 《Biochemical and biophysical research communications》1991,176(3):1443-1449
A cDNA clone encoding the N-terminal sequence of the murine integrin beta 7 subunit, a novel member of the leukocyte cell adhesion molecule subset (Leu-CAM), has been isolated. An N-terminal region of 13 contiguous amino acids deduced from the cDNA shows complete identity with the N-terminus of the 120 kDa subunit of the M290 antigen, a surface molecule found highly expressed on mouse intestinal intraepithelial lymphocytes (IEL). This unexpected result focuses two previously unconnected areas of research and suggests that integrins may have a special role to play in the defence of the gut mucosa. 相似文献
13.
14.
R. Watson 《BMJ (Clinical research ed.)》1997,314(7098):1854
15.
Corinna Richter Ron L. Dy Rebecca E. McKenzie Bridget N.J. Watson Corinda Taylor James T. Chang Matthew B. McNeil Raymond H.J. Staals Peter C. Fineran 《Nucleic acids research》2014,42(13):8516-8526
Clustered regularly interspaced short palindromic repeats (CRISPR), in combination with CRISPR associated (cas) genes, constitute CRISPR-Cas bacterial adaptive immune systems. To generate immunity, these systems acquire short sequences of nucleic acids from foreign invaders and incorporate these into their CRISPR arrays as spacers. This adaptation process is the least characterized step in CRISPR-Cas immunity. Here, we used Pectobacterium atrosepticum to investigate adaptation in Type I-F CRISPR-Cas systems. Pre-existing spacers that matched plasmids stimulated hyperactive primed acquisition and resulted in the incorporation of up to nine new spacers across all three native CRISPR arrays. Endogenous expression of the cas genes was sufficient, yet required, for priming. The new spacers inhibited conjugation and transformation, and interference was enhanced with increasing numbers of new spacers. We analyzed ∼350 new spacers acquired in priming events and identified a 5′-protospacer-GG-3′ protospacer adjacent motif. In contrast to priming in Type I-E systems, new spacers matched either plasmid strand and a biased distribution, including clustering near the primed protospacer, suggested a bi-directional translocation model for the Cas1:Cas2–3 adaptation machinery. Taken together these results indicate priming adaptation occurs in different CRISPR-Cas systems, that it can be highly active in wild-type strains and that the underlying mechanisms vary. 相似文献
16.
17.
18.
19.
20.
P A Walker H C Joao J A Littlechild R J Williams H C Watson 《European journal of biochemistry》1992,207(1):29-37
Site-directed mutagenesis has been used to produce mutant forms of yeast phosphoglycerate kinase in which the conserved active-site residue, Arg21, has been replaced by a methionine or a lysine. Kinetic results obtained using these mutant enzymes show that their Km for both 3-phospho-D-glycerate and ATP are significantly different from those recorded for the wild-type enzyme. The Vmax for the lysine mutant is reduced by a factor of two from that of the wild-type enzyme whereas the Vmax for the methionine mutant is reduced more than sevenfold. A very clean electron-density-difference map shows little, if any, evidence of a structural change associated with the C-terminal domain, although resonances in the NMR spectra associated with the ATP-binding site (C-terminal domain) are also affected by the mutation as one might expect from the kinetic results. The NMR data show that binding at both the 3-phospho-D-glycerate and the non-productive ATP-binding site (associated with the N-terminal domain) are affected in the mutant in a way which is different to that associated with the wild-type enzyme. These results, taken together with the X-ray and kinetic data, indicate that the non-productive ATP-binding site and the activating anion-binding site are both associated with the basic patch region of yeast phosphoglycerate kinase. 相似文献