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91.
We compared the richness and abundance of free-feeding herbivore insects (sap-sucking and leaf-chewing), leaf herbivory damage, leaf toughness and total phenolic content between two ontogenetic stages (juvenile and reproductive) of Handroanthus spongiosus (Rizzini) S. O. Grose (Bignoniaceae) throughout the rainy season in a Brazilian seasonally dry tropical forest. Twenty marked individuals of H. spongiosus were sampled per ontogenetic stage in each period of the rainy season (beginning, middle, and end). Herbivore richness and abundance did not differ between ontogenetic stages, but higher percentage of leaf damage, higher concentration of phenolic compounds, and lower leaf toughness were observed for juvenile individuals. The greatest morphospecies abundance was found at the beginning of the rainy season, but folivory increment was higher at the end, despite the fact that leaf toughness and total phenolic content increased in the same period. No significant relationships between leaf damage and both total phenolic content and leaf toughness were observed. These results suggest that insect richness and abundance do not track changes in foliage quality throughout plant ontogeny, but their decrease along rainy season confirms what was predicted for tropical dry forests. The general trends described in the current study corroborate those described in the literature about herbivores and plant ontogeny. However, the lack of relationship between herbivore damage and the two plant attributes considered here indicates that the analyses of multiple defensive traits (the defense syndrome) must be more enlightening to determine the mechanisms driving temporal and spatial patterns of herbivore attack. 相似文献
92.
da Costa da Santos EK Honda RT Nozawa SR Ferreira-Nozawa MS 《Genetics and molecular biology》2012,35(1):134-141
Analysis of bacterial diversity in soils along the banks of the Solimões and
Negro rivers, state of Amazonas, Brazil, was by partial sequencing of the genes codifying
the rDNA16S region. Diversity of operational taxonomic units (OTU) and of the divergent
sequences obtained were applied in comparative analysis of microbiological diversity in
the two environments, based on richness estimators and OTU diversity indices. The higher
OTU diversity in the Solimões was based on the higher number of parameters that
evoke this. The interaction between the nucleotide sequences of bacteria inhabiting the
two riverine environments indicated that the two microrganism communities are similar in
composition. 相似文献
93.
Clarissa X. R. Valim Luiz Roberto Basso Jr Fausto B. dos Reis Almeida Thaila Fernanda Reis André Ricardo Lima Damásio Luisa Karla Arruda Roberto Martinez Maria Cristina Roque-Barreira Constance Oliver Maria Célia Jamur Paulo Sergio Rodrigues Coelho 《PloS one》2012,7(9)
Paracoccidioides brasiliensis is the etiologic agent of paracoccidioidomycosis (PCM), one of the most prevalent mycosis in Latin America. P. brasiliensis cell wall components interact with host cells and influence the pathogenesis of PCM. Cell wall components, such as glycosylphosphatidylinositol (GPI)-proteins play a critical role in cell adhesion and host tissue invasion. Although the importance of GPI-proteins in the pathogenesis of other medically important fungi is recognized, little is known about their function in P. brasiliensis cells and PCM pathogenesis. We cloned the PbPga1 gene that codifies for a predicted GPI-anchored glycoprotein from the dimorphic pathogenic fungus P. brasiliensis. PbPga1 is conserved in Eurotiomycetes fungi and encodes for a protein with potential glycosylation sites in a serine/threonine-rich region, a signal peptide and a putative glycosylphosphatidylinositol attachment signal sequence. Specific chicken anti-rPbPga1 antibody localized PbPga1 on the yeast cell surface at the septum between the mother cell and the bud with stronger staining of the bud. The exposure of murine peritoneal macrophages to rPbPga1 induces TNF-α release and nitric oxide (NO) production by macrophages. Furthermore, the presence of O-glycosylation sites was demonstrated by β-elimination under ammonium hydroxide treatment of rPbPga1. Finally, sera from PCM patients recognized rPbPga1 by Western blotting indicating the presence of specific antibodies against rPbPga1. In conclusion, our findings suggest that the PbPga1gene codifies for a cell surface glycoprotein, probably attached to a GPI-anchor, which may play a role in P. brasiliensis cell wall morphogenesis and infection. The induction of inflammatory mediators released by rPbPga1 and the reactivity of PCM patient sera toward rPbPga1 imply that the protein favors the innate mechanisms of defense and induces humoral immunity during P. brasiliensis infection. 相似文献
94.
Miguel E. Arechavaleta-Velasco Karla Alcala-Escamilla Carlos Robles-Rios Jennifer M. Tsuruda Greg J. Hunt 《PloS one》2012,7(11)
Populations of honey bees in North America have been experiencing high annual colony mortality for 15–20 years. Many apicultural researchers believe that introduced parasites called Varroa mites (V. destructor) are the most important factor in colony deaths. One important resistance mechanism that limits mite population growth in colonies is the ability of some lines of honey bees to groom mites from their bodies. To search for genes influencing this trait, we used an Illumina Bead Station genotyping array to determine the genotypes of several hundred worker bees at over a thousand single-nucleotide polymorphisms in a family that was apparently segregating for alleles influencing this behavior. Linkage analyses provided a genetic map with 1,313 markers anchored to genome sequence. Genotypes were analyzed for association with grooming behavior, measured as the time that individual bees took to initiate grooming after mites were placed on their thoraces. Quantitative-trait-locus interval mapping identified a single chromosomal region that was significant at the chromosome-wide level (p<0.05) on chromosome 5 with a LOD score of 2.72. The 95% confidence interval for quantitative trait locus location contained only 27 genes (honey bee official gene annotation set 2) including Atlastin, Ataxin and Neurexin-1 (AmNrx1), which have potential neurodevelopmental and behavioral effects. Atlastin and Ataxin homologs are associated with neurological diseases in humans. AmNrx1 codes for a presynaptic protein with many alternatively spliced isoforms. Neurexin-1 influences the growth, maintenance and maturation of synapses in the brain, as well as the type of receptors most prominent within synapses. Neurexin-1 has also been associated with autism spectrum disorder and schizophrenia in humans, and self-grooming behavior in mice. 相似文献
95.
96.
97.
98.
Carl N. McDaniel Laura K. Hartnett Karla A. Sangrey 《The Plant journal : for cell and molecular biology》1996,9(1):55-61
Employing genotypes of day-neutral tobacco that exhibit a wide range in the number of nodes produced, it has been established that node number, and plant size, in tobacco is regulated, in large part, by two endogenous signals and one developmental state, competence. All genotypes have the same level of a root signal that maintains a vegetative pattern during early growth. The number of nodes produced before the formation of the terminal flower, as well as plant size, is a function of the strength of the floral stimulus from the leaves and the competence of the terminal meristem to respond to the floral stimulus by initiating the terminal flower. 相似文献
99.
Karla Jean Fullner 《Journal of bacteriology》1998,180(2):430-434
Nonpolar virB mutants of Agrobacterium tumefaciens were tested for RSF1010 mobilization and extracellular complementation. virB2 to virB11 were essential for transfer in both assays. virB1 was essential only for high frequency transfer of RSF1010 and VirE2. Coordinated transfer of a preassembled T complex is supported by these data and competition studies. 相似文献
100.
Karpe A. V. Dunn M. S. Taylor M. C. Nguyen T. Ong C. Karla T. Rockman S. Beale D. J. 《Metabolomics : Official journal of the Metabolomic Society》2018,14(12):1-1
Metabolomics - The identification of metabolomic dysregulation appears promising for the prediction of type 1 diabetes and may also reveal metabolic pathways leading to beta-cell destruction.... 相似文献