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91.
Host cell factor and an uncharacterized SANT domain protein are stable components of ATAC, a novel dAda2A/dGcn5-containing histone acetyltransferase complex in Drosophila
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Guelman S Suganuma T Florens L Swanson SK Kiesecker CL Kusch T Anderson S Yates JR Washburn MP Abmayr SM Workman JL 《Molecular and cellular biology》2006,26(3):871-882
Gcn5 is a conserved histone acetyltransferase (HAT) found in a number of multisubunit complexes from Saccharomyces cerevisiae, mammals, and flies. We previously identified Drosophila melanogaster homologues of the yeast proteins Ada2, Ada3, Spt3, and Tra1 and showed that they associate with dGcn5 to form at least two distinct HAT complexes. There are two different Ada2 homologues in Drosophila named dAda2A and dAda2B. dAda2B functions within the Drosophila version of the SAGA complex (dSAGA). To gain insight into dAda2A function, we sought to identify novel components of the complex containing this protein, ATAC (Ada two A containing) complex. Affinity purification and mass spectrometry revealed that, in addition to dAda3 and dGcn5, host cell factor (dHCF) and a novel SANT domain protein, named Atac1 (ATAC component 1), copurify with this complex. Coimmunoprecipitation experiments confirmed that these proteins associate with dGcn5 and dAda2A, but not with dSAGA-specific components such as dAda2B and dSpt3. Biochemical fractionation revealed that ATAC has an apparent molecular mass of 700 kDa and contains dAda2A, dGcn5, dAda3, dHCF, and Atac1 as stable subunits. Thus, ATAC represents a novel histone acetyltransferase complex that is distinct from previously purified Gcn5/Pcaf-containing complexes from yeast and mammalian cells. 相似文献
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Dagmar Wilhelm Linda L. Washburn Vy Truong Marc Fellous Eva M. Eicher Peter Koopman 《Mechanisms of development》2009,126(5-6):324-336
Ovotestis development in B6-XYPOS mice provides a rare opportunity to study the interaction of the testis- and ovary-determining pathways in the same tissue. We studied expression of several markers of mouse fetal testis (SRY, SOX9) or ovary (FOXL2, Rspo1) development in B6-XYPOS ovotestes by immunofluorescence, using normal testes and ovaries as controls. In ovotestes, SOX9 was expressed only in the central region where SRY is expressed earliest, resulting in testis cord formation. Surprisingly, FOXL2-expressing cells also were found in this region, but individual cells expressed either FOXL2 or SOX9, not both. At the poles, even though SOX9 was not up-regulated, SRY expression was down-regulated normally as in XY testes, and FOXL2 was expressed from an early stage, demonstrating ovarian differentiation in these areas. Our data (1) show that SRY must act within a specific developmental window to activate Sox9; (2) challenge the established view that SOX9 is responsible for down-regulating Sry expression; (3) disprove the concept that testicular and ovarian cells occupy discrete domains in ovotestes; and (4) suggest that FOXL2 is actively suppressed in Sertoli cell precursors by the action of SOX9. Together these findings provide important new insights into the molecular regulation of testis and ovary development. 相似文献
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David G. Washburn Tram H. Hoang James S. Frazee Latisha Johnson Marlys Hammond Sharada Manns Kevin P. Madauss Shawn P. Williams Chaya Duraiswami Thuy B. Tran Eugene L. Stewart Eugene T. Grygielko Lindsay E. Glace Walter Trizna Rakesh Nagilla Jeffrey D. Bray Scott K. Thompson 《Bioorganic & medicinal chemistry letters》2009,19(16):4664-4668
We have designed and synthesized a novel series of pyrrolidinones as progesterone receptor partial agonists. Compounds from this series had improved AR selectivity, rat pharmacokinetic properties, and in vivo potency compared to the lead compound. In addition, these compounds had improved selectivity against hERG channel inhibition. 相似文献
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Mihaela E. Sardiu Joshua M. Gilmore Michael J. Carrozza Bing Li Jerry L. Workman Laurence Florens Michael P. Washburn 《PloS one》2009,4(10)
Protein complexes are key molecular machines executing a variety of essential cellular processes. Despite the availability of genome-wide protein-protein interaction studies, determining the connectivity between proteins within a complex remains a major challenge. Here we demonstrate a method that is able to predict the relationship of proteins within a stable protein complex. We employed a combination of computational approaches and a systematic collection of quantitative proteomics data from wild-type and deletion strain purifications to build a quantitative deletion-interaction network map and subsequently convert the resulting data into an interdependency-interaction model of a complex. We applied this approach to a data set generated from components of the Saccharomyces cerevisiae Rpd3 histone deacetylase complexes, which consists of two distinct small and large complexes that are held together by a module consisting of Rpd3, Sin3 and Ume1. The resulting representation reveals new protein-protein interactions and new submodule relationships, providing novel information for mapping the functional organization of a complex. 相似文献
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Lisa Kutschbach-Brohl Brian E. Washburn Glen E. Bernhardt Richard B. Chipman Laura C. Francoeur 《Journal of Insect Conservation》2010,14(4):347-358
Semi-natural grassland habitat fragments, such as those found on airports, might be important for arthropod conservation and
biodiversity in urban ecosystems. The objectives of this study were to: (1) describe the arthropod communities present within
the grasslands on the John F. Kennedy International Airport and (2) assess spatial and temporal variation in those arthropod
communities. We collected arthropods using a vacuum sampler during 2003 and using sweep-net collection methods during 2003
and 2004. During 2003, a total of 1,467 arthropods, representing 17 orders and 68 families were found in vacuum samples. A
total of 3,784 arthropods, representing 12 orders and 94 families were collected in sweep-net samples during 2003. In 2004,
a total of 3,281 arthropods, representing 12 orders and 85 families were collected in sweep-net samples. Hemiptera, Orthoptera,
and Diptera were the most abundant taxa, accounting for 47, 18, and 14% of all arthropods captured, respectively. We found
evidence of spatial and temporal variation in arthropod abundance, in particular as noted by fluctuations in Orthoptera: Acrididae
and Hemiptera: Auchenorrhyncha. Hemipteran family diversity was also influenced by habitat type. Grassland habitats on airfields,
although influenced by anthropogenic factors (e.g., mowing), have the potential to provide abundant and diverse arthropod
communities and might serve as a refugium for such species within urban ecosystems. 相似文献
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Márcia R Soares Agda P Facincani Rafael M Ferreira Leandro M Moreira Julio CF de Oliveira Jesus A Ferro Maria IT Ferro Rogério Meneghini Fábio C Gozzo 《Proteome science》2010,8(1):55