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41.
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The in vivo incorporation of linolenic acid into neuronal and glial cells and myelin 总被引:6,自引:5,他引:1
Abstract— Neurons, astrocytes, oligodendrocytes, and myelin were prepared from 21-day-old rat brain at various times after intracerebral injection of [1-14C]linolenate. Comparisons of phospholipid specific radioactivity demonstrated that the oligodendrocytes were much more active than neuronal, astroglial, or myelin fractions. This is consistent with the concept that the oligodendrocyte is responsible for synthesis of the relatively large mass of myelin sheath. Initially the phosphatidylcholine fraction was more active than the phosphatidylethanolamine fraction, but during the 36 h after injection the former decreased in radioactivity while the latter fraction showed an increase. Fatty acid elongation occurred rapidly. Within 2h after injection, 2/3 of the label had been converted to elongated products (20:4. 20:5, 22:5 and 22:6). All three cell types apparently contained the enzymes necessary to incorporate, elongate, and desaturate linolenic acid and this occurred at similar rates in each cell type. No direct precursor-product relationship was found between the lipids of oligodendrocytes and myelin. There was, however, a lag in the appearance of elongated fatty acids in the phosphoglycerides of myelin. indicating that the polyunsaturated fatty acids in myelin were synthesized elsewhere and transported into the myelin sheath. 相似文献
44.
Clonal abortion of bone marrow T cell precursors: T cells acquire specific antigen reactivity prethymically 总被引:1,自引:0,他引:1
R Chervenak J J Cohen S D Miller 《Journal of immunology (Baltimore, Md. : 1950)》1983,131(4):1688-1692
Lethally irradiated (900 R) mice were reconstituted with bone marrow cells from syngeneic donors that had been tolerized 2 to 3 wk earlier to either DNP or TNP compounds. Five weeks after reconstitution, these animals were tested for their ability to mount a delayed hypersensitivity (DH) response to the tolerizing haptens. Recipient mice were specifically tolerant to the hapten that was used to induce tolerance in the marrow donor. Mixing experiments in which mice were reconstituted with marrow from DNP-tolerant and TNP-tolerant donors showed no indication of active suppression or effective antigen carry-over in this system. This observation held true even in experiments in which mice were reconstituted with a mixture of marrow from tolerant and normal donors at a ratio of 5:1. Thus the reduced responsiveness in recipient mice seemed to be due to the functional elimination of hapten-responsive T cell precursor (pre-T) clones. Recipient unresponsiveness was also shown to be MHC restricted. Maintenance of unresponsiveness appeared to be due to the restricted access of regenerating pre-T cell clones to the maturational influence of the recipient's thymus. 相似文献
45.
A. Cohen A. M. Breure D. J. M. Schmedding R. J. Zoetemeyer J. G. van Andel 《Applied microbiology and biotechnology》1985,21(6):404-408
Summary The effect of partial pre-acidification of carbohydrate containing wastewaters on anaerobic digester performance was investigated. The influent was a 1% (w/v) glucose solution in a mineral salts medium imposing carbon-limited growth conditions. Up to 13% of the Chemical Oxygen Demand (COD) was added as volatile fatty acids (VFA).In all cases, addition of VFA to the glucose medium resulted in significant increases in the maximum specific COD-conversion rates of the sludge (both with respect to continuous feeding and following a shock loading), as compared with values found on digestion of glucose media alone. 相似文献
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David L Cohen 《BMJ (Clinical research ed.)》1985,291(6506):1428
48.
Ninety-one patients who had had augmentation mammaplasties were evaluated retrospectively. The intra-implant use of steroids appeared to reduce the incidence of breast firmness--at least over the short follow-up period--but there were complications from this which appear to be dose related (mg of Solu-Medrol/100 cc saline). A long-term evaluation of such patients is desirable. Meanwhile, our limited data strongly suggest that the intra-implant use of steroids should be used only with caution as to the dose and with a careful follow-up. 相似文献
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In this paper we report the observation of a rapidly developed vertebrate symbiosis involving ectoparasite cleaning by a native corvid of northern Australia, the Torresian crow Corvus orru , on a recently introduced bovid ungulate, the banteng Bos javanicus . Facultative symbioses benefiting both participants (mutualisms) between birds and mammals appear to be rare, despite the apparent advantages obtained by the participants (ecto-parasite removal from the host and food provision to the cleaner). On three separate dates we observed a total of four C. orru individuals eliciting facilitation behaviours by a total of ten female banteng to assist in the removal of ectoparasites. Our observations document the first-known incidence of facultative cleaning behaviour by a native bird species on a non-native, wild vertebrate that has developed in approximately 150 years since the banteng's introduction to Australia. 相似文献