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Theerachat M Virunanon C Chulalaksananukul S Sinbuathong N Chulalaksananukul W 《World journal of microbiology & biotechnology》2011,27(4):999-1003
The genetic heterogeneity of the nitrite reductase gene (nirK and nirS) fragments from denitrifying prokaryotes in a non-agricultural forest soil in Thailand was investigated using soil samples
from the Plant Germplasm-Royal Initiation Project area in Kanchanaburi Province, Thailand. Soil bacteria were screened for denitrification activity and 13 (from 211) positive isolates were obtained and further evaluated
for their ability to reduce nitrate and to accumulate or reduce nitrite. Three species with potentially previously unreported
denitrifying activities were recorded. Analysis of the partial nirK and nirS sequences of these 13 strains revealed a diverse sequence heterogeneity in these two genes within the same environment and
even potentially within the same host species, the potential existence of lateral gene transfer and the first record of both
nirK and nirS homologues in one bacterial species. Finally, isolates of two species of bacteria (Corynebacterium propinquum and Micrococcus lylae) are recorded as denitrifiers for the first time. 相似文献