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961.
记述2个新遗迹属──Biconcavichnus和Fasciarichnus,并讨论了它们的形成方式和形成环境。化石产于贵阳市南约20km的孟关附近的下三叠统安顺组上部。 相似文献
962.
Three group 10 complexes containing nido-carborane diphosphine, [NiCl(PPh3){7,8-(PPh2)2-7,8-C2B9H10}] (1), [PdCl(PPh3){7,8-(PPh2)2-7,8-C2B9H10}] · 1.25CH2Cl2 (2) and [PtCl(PPh3){7,8-(PPh2)2-7,8-C2B9H10}] · 2.5CH2Cl2 (3) have been synthesized by the reactions of [M(PPh3)2Cl2] (M = Ni, Pd, Pt) with closo carborane diphosphine 1,2-(PPh2)2-1,2-C2B10H10 in ethanol. For complex 3, it could also be obtained under solvothermal condition. All three complexes were characterized by elemental analysis, FT-IR, 1H and 13C NMR spectroscopy and X-ray structure determination. Single crystal structures show that their structures are similar to each other. In each complex, the nido [7,8-(PPh2)2-7,8-C2B9H10]−, which resulted from the degradation of the initial closo ligand 1,2-(PPh2)2-1,2-C2B10H10 during the reaction process, was coordinated bidentately through the P atoms to M(II) ion, and this resulted in a stable five-membered chelating ring between the bis-diphosphine ligand and the metal. The coordination mode of the metal can be described as a slightly distorted square-planar, in which the remaining two positions were occupied by one Cl− and one PPh3 group. 相似文献
963.
964.
According to the ultrastructural characteristic observation of the developing male germ cells, spermatogenesis of the crustacean
shrimp, Fenneropenaeus chinensis, is classified into spermatogonia, primary spermatocytes, secondary spermatocyte, four stages of spermatids, and mature sperm.
The basic protein transition during its spermatogenesis is studied by transmission electron microscopy of ammoniacal silver
reaction and immunoelectron microscopical distribution of acetylated histone H4. The results show that basic protein synthesized
in cytoplasm of spermatogonia is transferred into the nucleus with deposition on new duplicated DNA. In the spermatocyte stage,
some nuclear basic protein combined with RNP is transferred into the cytoplasm and is involved in forming the cytoplasmic
vesicle clumps. In the early spermatid, most of the basic protein synthesized in the new spermatid cytoplasm is transferred
into the nucleus, and the chromatin condensed gradually, and the rest is shifted into the pre-acrosomal vacuole. In the middle
spermatid, the nuclear basic protein linked with DNA is acetylated and transferred into the proacrosomal vacuole and assembled
into the acrosomal blastema. At the late spermatid, almost all of the basic protein in the nucleus has been removed into the
acrosome. During the stage from late spermatid to mature sperm, some de novo basic proteins synthesized in the cytoplasm belt
transfer into the nucleus without a membrane and almost all deposit in the periphery to form a supercoating. The remnant histone
H4 accompanied by chromatin fibers is acetylated in the center of the nucleus, leading to relaxed DNA and activated genes
making the nucleus non-condensed. 相似文献
965.
Ding Haixia Zhang Jingsong Jiang Lei Dong Hairong Wang Jun Xiao Hang Chen Weixian 《Cell biochemistry and biophysics》2011,59(1):39-47
Extracellular domains of the transmembrane glycoprotein, neuropilin-1 (Np1), specifically bind an array of factors and co-receptors
including class-3 semaphorins (Sema3a), vascular endothelial growth factor (VEGF), hepatocyte growth factor, platelet-derived
growth factor BB, transforming growth factor-β 1 (TGF-β1), and fibroblast growth factor2 (FGF2). Np1 may have a role in immune
response, tumor cell growth, and angiogenesis, but its relative expression in comparison to its co-primary receptors, VEGF
and Sema3a, is not known. In this study we determined the mRNA expression of Np1 and its co-receptors, VEGF and Sema3a, and
the ratio of VEGF/Sema3a in different human and rodent cell lines. Expression of Np1, VEGF and Sema3a is very low in cells
derived from normal tissues, but these proteins are highly expressed in tumor-derived cells. Furthermore, the ratio of VEGF/Sema3a
is highly variable in different tumor cells. The elevated mRNA expression of Np1 and its putative receptors in tumor cells
suggests a role for these proteins in tumor cell migration and angiogenesis. As different tumor cells exhibit varying VEGF/Sema3a
ratios, it appears that cancer cells show differential response to angiogenic factors. These results bring to light the individual
variation among the cancer-related genes, Np1, VEGF, and Sema3a, and provide an important impetus for the possible personalized
therapeutic approaches for cancer patients. 相似文献
966.
967.
Li C Wang X Wang G Wu C Li N 《Comparative biochemistry and physiology. Part D, Genomics & proteomics》2011,6(3):264-270
Roxarsone is a commonly used additive in chicken (Gallus gallus) industry. However, little is known on the intrinsic molecular mechanism via which the growth performance of birds improves. This study was therefore performed to investigate the expression profiles of genes induced by roxarsone. Fifty-six broiler chickens were divided into two groups, namely treated and untreated with roxarsone. The treated group was provided a diet of 45.4 mg/kg roxarsone medication and the other group acted as control. Data analysis showed that roxarsone consistently and significantly (P < 0.05) increased chicken growth performance. In addition to this a significant (P < 0.05) increase of arsenic residue in liver has been seen. Microarray expression analysis of 8935 genes in liver showed that 22 genes (10 up- and 12 down-regulated) had altered expression throughout the experimental periods. Two novel genes (GenBank accession no. GU724343 and GU724344) were cloned through rapid amplification of cDNA ends (RACE). Gene GU724343 was predicted to encode an unidentified protein and the second gene GU724344 was presumed to encode a new member of immunoglobulin-like receptor (CHIR) family. Our results suggested for the first time that the role of roxarsone could be mainly to modify the expression levels of cell growth, immunity/defense and energy metabolism associated genes, as a result promoting animal growth. Further research on these genes should help to increase the knowledge of improving animal productivity safely and effectively. 相似文献
968.
Po-Chun Chang Chun-Jen Wang Chung-Kai You Mou-Chieh Kao 《Biochemical and biophysical research communications》2011,(3):1641
Lipopolysaccharide (LPS) is considered as an important virulence factor of Helicobacter pylori, and contributes to infection persistence and disease severity. ADP-l-glycero-d-manno-heptose-6-epimerase is an enzyme essential for LPS synthesis and understanding of its biochemistry is critical for drug development. We cloned one putative ortholog of Escherichia colirfaD, HP0859, from H. pylori 26695. Determination of the native molecular weight of the recombinant HP0859 protein suggests that the protein is likely a hexamer. NADP+, instead of NAD+, was proved to be the physiological cofactor for HP0859 protein. Circular dichroism spectrum analysis demonstrated that the secondary structure of this protein is significantly altered when the cofactor is removed. We also constructed an HP0859 knockout mutant and examined its phenotypic properties. The HP0859 knockout mutant exhibited a severe truncation of LPS, a decreased growth rate, and a higher susceptibility to novobiocin. Disruption of HP0859 also reduced the adhesive capacity of H. pylori to AGS cells, and the infected cells failed to display the classic hummingbird phenotype. Complementation of the HP0859 knockout mutation restored these phenotypes completely. In conclusion, we demonstrate that HP0859 codes for a protein essential for the LPS inner core biosynthesis in H. pylori and an intact LPS structure contributes to the adherence ability of this bacterium. 相似文献
969.
970.
Livia Garzia Noriyuki Kijima A. Sorana Morrissy Pasqualino De Antonellis Ana Guerreiro-Stucklin Borja L. Holgado Xiaochong Wu Xin Wang Michael Parsons Kory Zayne Alex Manno Claudia Kuzan-Fischer Carolina Nor Laura K. Donovan Jessica Liu Lei Qin Alexandra Garancher Kun-Wei Liu Michael D. Taylor 《Cell》2018,172(5):1050-1062.e14