Localization of the gene encoding human Factor V to chromosome 1q21–25

The gene encoding human coagulation Factor V (FV), one of the cofactors in the blood clotting process, has been mapped to chromosome 1 by both Southern hybridization to DNA from human-hamster somatic cell hybrids and in situ hybridization. The whole plasmid pUC3A containing a 1.5-kb cDNA sequence for FV was 32P-labeled for Southern analysis and 3H-labeled for in situ hybridization to metaphase chromosomes. The results localized the FV gene to the region of 1q21-25.     

我国浙江与日本和我国台湾森林植物区系的联系

作者在“试论浙江省森林植物区系”一文中对我国浙江与日本和我国台湾森林植物区系的联系已作了扼要的说明,本文就是在此基础上作更详尽的论述。浙江省东濒大海,隔海相望,东北方向有日本,其本部岛屿的南端为九州,和浙江北部的纬度相近,具有亚热带气候特征,出现了和浙江相类似的常绿阔叶林和竹林,九     

九江地区人头发横断面的研究

本文对九江市汉族463例健康人,4710根头发横断面进行观察和显微测量。分析了本地区人年龄与头发直径的关系、年龄与头发黑色素颗粒分布的关系。结果表明:黑色素颗粒随年龄增长而渐浓密,成年人最丰富,老年人头发黑色素颗粒则渐崩溃稀少;头发各径亦随年龄增长而增大,而老年人头发各径是逐渐减小。此外,本文还同欧、亚、美国各民族人头发资料进行比较。根据头发指数,确定本市人头发形伏为直发形。     

用体外免疫法建立人—人抗流行性乙型脑炎病毒杂交瘤细胞株

利用单克隆抗体(McAb)进行病毒病的治疗是人们所关心的一个重大课题。 流行性乙型脑炎(乙脑)是一种严重威胁人民健康的急性传染病,病死率高,后遗症严重。国内外目前尚无特效疗法。陈伯权等用乙脑病毒皮下或腹腔感染3周龄小白鼠24、48小时及5天后,分别用乙脑病毒51-8McAb进行治疗,平均治愈率分别为78％、73％及22％。     

小鼠Lewis肺癌组织中层粘连蛋白受体的分离及其性质的研究

层粘连蛋白(Laminin,LN)是基膜(basement membrane)中的一种主要大分子糖蛋白。一些研究资料表明肿瘤细胞的浸润转移可能与LN有关。肿瘤细胞与LN的作用可能是通过细胞表面LN受体进行的。本文采用亲和层析法从小鼠Lewis肺癌组织中分离LN受体并对其理化性质进行研究。Lewis肺癌LN受体的表观分子量为70,000,还原后SDS电泳图为一条较宽的条带。氨基酸组成中疏水氨基酸占38％,苏氨酸、絲氨酸、门冬氨酸(包括门冬酰胺)占23.5％,通过硝酸纤维素膜片法用HRP-LN测定受体与LN的结合特性,证明具有配基结合专一性,饱和性及高亲和性(Kd=0.95×10~(-9)mol/L)。     

Alternate use of divergent forms of an ancient exon in the fructose-1,6-bisphosphate aldolase gene of Drosophila melanogaster.

The fructose-1,6-bisphosphate aldolase gene of Drosophila melanogaster contains three divergent copies of an evolutionarily conserved 3' exon. Two mRNAs encoding aldolase contain three exons and differ only in the poly(A) site. The first exon is small and noncoding. The second encodes the first 332 amino acids, which form the catalytic domain, and is homologous to exons 2 through 8 of vertebrates. The third exon encodes the last 29 amino acids, thought to control substrate specificity, and is homologous to vertebrate exon 9. A third mRNA substitutes a different 3' exon (4a) for exon 3 and encodes a protein very similar to aldolase. A fourth mRNA begins at a different promoter and shares the second exon with the aldolase messages. However, two exons, 3a and 4a, together substitute for exon 3. Like exon 4a, exon 3a is homologous to terminal aldolase exons. The exon 3a-4a junction is such that exon 4a would be translated in a frame different from that which would produce a protein with similarity to aldolase. The putative proteins encoded by the third and fourth mRNAs are likely to be aldolases with altered substrate specificities, illustrating alternate use of duplicated and diverged exons as an evolutionary mechanism for adaptation of enzymatic activities.     

电损毁海马CA3区及连合前穹窿对大鼠血浆胰岛素水平...

Bilateral electrical lesioning of the hippocampal CA3 region (HCA3-EL) or anterior commissura hippocampi (ACHF-EL) caused marked elevations in plasma basal levels of insulin. 2 weeks later, fasting blood glucose levels were also augmented with decreased glucose tolerance. In contrast, the secretory response of pancreatic B cells to glucose stimulation was markedly enhanced. Following intravenous glucose tolerance test (IVGTT), the relative amounts of glucagon-like and insulin-like immunoreactants were reduced in the pancreatic islets of both HCA3-EL and ACHF-EL rats in comparison with the controls. In the HCA3-EL group, the relative amounts of somatostatin-like immunoreactants and gross numbers of such immunostained cells in islets were also decreased as compared with the control. No difference was seen in pancreatic-polypeptide-like immunoreactivities as assessed by immunohistochemistry plus microphotometry method. The above results suggest strongly that HCA3 and ACHF exert a tonic inhibitory action on the insulin secretion in the rat.     

Age, gender and circadian or circasemidian blood pressure and heart rate variation of children.

Systolic (S) and diastolic (D) blood pressure (BP) and heart rate (HR) of clinically healthy children (24 boys and 15 girls) 3 to 7 years of age were measured with a standard mercury sphygmomanometer at 3-hour intervals for 24 hours in April 1991. The children slept and/or rested from 2100 to 0700 and napped from 1230 to 1530; they had meals at 0730, 1200 and 1800. A statistically significant circadian and about 12-hour (circasemidian) component of variation is documented for SBP and DBP of boys and girls and for HR of boys. No gender difference was found for the circadian and circasemidian components. A positive correlation with age is found for the MESOR and circadian amplitude of SBP and DBP (p < 0.05); a negative correlation with age is found for the MESOR of HR (p < 0.001).