Isolation of specific antigens from Angiostrongylus cantonensis. 2. By affinity chromatography

The crude adult worm extract of Angiostrongylus cantonensis was subjected to a series of affinity chromatography for selective removal of host antigens as well as cross-reactive components with other helminths. The purified fraction designated as AC(p) with molecular weights ranging from 10,000-42,000 was found to contain antigenic components specific to A. cantonensis as determined by immunoelectrophoresis and enzyme-linked immunosorbent assay.     

Lactobacillus plantarum lipoteichoic acid disrupts mature Enterococcus faecalis biofilm

Journal of Microbiology - Apical periodontitis is caused by biofilm-mediated root canal infection. Early phase oral bacterial biofilms are inhibited by Lactobacillus plantarum lipoteichoic acid...     

Optimal conditions for the production of exopolysaccharide by marine microorganismHahella chejuensis

A marine microorganism, strain 96CJ10356 produced exopolysaccharide, designated as EPS-R. To optimize culture conditions for the production of EPS-R, carbon and nitrogen sources, mineral salts, temperature, and pH were examined. From this study, STN medium for the production of EPS-R was suggested as follows; sucrose 20 g, tryptone 10 g, NaCl 10 g, MgSO₄ 5 g, CaCl₂ 1 g, KH₂PO₄ 76 mg, K₂HPO₄ 83 mg, FeCl₂ 5 mg, MnCl₂ 1 mg, NaMoO₄ 1 mg, and ZnCl₂ 1 mg per liter at pH 7.0. About 9.23 g/L of EPS-R was obtained from STN medium after cultivation for 120 h at 25°C in a 5-liter jar fermentor with an aeration rate of 0.17 vvm. Apparent viscosity and flocculation activity of the culture broth were increased with the production of EPS-R and the maximal values were 415 cP and 1400 unit/mL against 0.5% activated carbon, respectively.