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101.
Predictive model of conjugative plasmid transfer in the rhizosphere and phyllosphere. 总被引:4,自引:3,他引:1 下载免费PDF全文
G R Knudsen M V Walter L A Porteous V J Prince J L Armstrong R J Seidler 《Applied microbiology》1988,54(2):343-347
A computer simulation model was used to predict the dynamics of survival and conjugation of Pseudomonas cepacia (carrying the transmissible recombinant plasmid R388:Tn1721) with a nonrecombinant recipient strain in simple rhizosphere and phyllosphere microcosms. Plasmid transfer rates were derived for a mass action model, and donor and recipient survival were modeled as exponential growth and decay processes or both. Rate parameters were derived from laboratory studies in which donor and recipient strains were incubated in test tubes with a peat-vermiculite solution or on excised radish or bean leaves in petri dishes. The model predicted donor, recipient, and transconjugant populations in hourly time steps. It was tested in a microcosm planted with radish seeds and inoculated with donor and recipient strains and on leaf surfaces of radish and bean plants also growing in microcosms. Bacteria were periodically enumerated on selective media over 7 to 14 days. When donor and recipient populations were 10(6) to 10(8) CFU/g (wet weight) of plant or soil, transconjugant populations of about 10(1) to 10(4) were observed after 1 day. An initial rapid increase and a subsequent decline in numbers of transconjugants in the rhizosphere and on leaf surfaces were correctly predicted. 相似文献
102.
Aerial Dispersal and Epiphytic Survival of Pseudomonas syringae during a Pretest for the Release of Genetically Engineered Strains into the Environment 总被引:15,自引:15,他引:0 下载免费PDF全文
Steven E. Lindow Guy R. Knudsen Ramon J. Seidler Michael V. Walter Victor W. Lambou Penny S. Amy David Schmedding Valerie Prince Stephen Hern 《Applied microbiology》1988,54(6):1557-1563
Prospective experimental field evaluation of genetically engineered microorganisms, such as microbial pest control agents, raises issues of how to properly ascertain their fate and survival in the environment. Field trials with recombinant organisms must reflect requirements for sampling and monitoring. Field trials were conducted at Tulelake, Calif., to monitor the numbers of viable cells of a nonrecombinant strain of Pseudomonas syringae that entered the atmosphere and landed on plants and soil during and after an aerosol spray application. An exponential decrease in numbers of viable cells deposited at increasing distances from three sprayed plots was observed. The relative rate of survival of cells sprayed directly on plants was more than 10 times higher than that of cells dispersed through the air to similar adjacent plants. Results are being used to gain experience with the characteristics of a release site that influence containment or dispersal and to develop appropriate sampling methodologies for evaluating survival and dispersal characteristics of genetically engineered bacteria released into the environment. The ability to make predictions about microbial dispersal and survival will reduce the uncertainties associated with environmental releases of recombinant organisms. 相似文献
103.
Miconia zanonii, which occurs in broad-leaved cloud forests at four localities in the Cordillera Central of the Dominican Republic, is described
and illustrated. It is compared withM. krugii andM. samanensis, two probably close relatives withinMiconia sect.Chaenopleura. 相似文献
104.
Walter Steffen 《Chromosoma》1986,94(2):107-114
A technique for the fixation of cells during live observation (Nicklas et al. 1979) was used to investigate chromosomes which were moving at the time of fixation. Chromosome fibres were reconstructed by tracking their microtubules in longitudinal serial sections. A considerable proportion of non-kinetochoric microtubules (free microtubules, fMTs) is skewed with respect to the fibre axis. These skew fMTs contribute to the degree of disorder. It was found that the difference in the relative proportion of skew fMTs between active fibres (oriented in the direction of movement) and passive fibres (oriented backwards) is significantly correlated with the chromosome velocity (correlation coefficient r=0.796, P=0.01). It can be concluded that the pulling force generated in the chromosome fibre is a function of skew fMTs. 相似文献
105.
Summary We report the successful mutagenesis of Azospirillum brasilense 29710 Rif Sm with transposon Tn5. The narrow host-range plasmid pGS9 (p15A replicon), which possesses broad host-range N-type transfer genes, was used as the suicide vehicle to deliver Tn5 in Azospirillum. Out of 900 colonies tested, 0.8% proved to be auxotrophic. One mutant altered in indoleacetic acid (auxin) biosynthesis was isolated and, in addition, three mutants completely defective in nitrogen fixation (nif) were obtained. All the mutants tested contained a single copy of Tn5 integrated randomly in the genome. The Tn5-mutagenized EcoRI fragments were cloned from the three Nif- mutants. Physical analysis of cloned DNA showed that Tn5 was present on a different EcoRI fragment in each case, ranging in size from 15–17 kb. The nitrogenase structural genes (nifHDK) in A. brasilense 29710 Rif Sm were localized on a 6.7 kb EcoRI fragment. We found that Tn5 is not inserted in the nifHDK genes in the Nif- mutants reported here. Site-directed mutagenesis using the cloned, Tn5-containing DNA from mutant Nif27(pMS188), produced a large number of Nif- transconjugants of the A. brasilense 29710 Rif wild-type strain, showing the linkage between Tn5 insertion and the Nif- phenotype. This is the first time that transposon-mutagenized auxotrophic, Nif- and other mutants have been available for genetic analysis in Azospirillum. This should greatly facilitate the cloning and mapping of genes involved in nitrogen fixation as well as in many other phenotypic characteristics of Azospirillum. 相似文献
106.
107.
Effect of glycerol on plasmid transfer in genetically competent Haemophilus influenzae 总被引:12,自引:0,他引:12
Summary The small plasmid pAT4 transformed at characteristically low frequencies those competent Haemophilus influenzae Rd strains that had no DNA homology with this plasmid. Transformation was increased up to 100 times, however, when the recipient cells were exposed to 30% glycerol before plating for transformants. Expression of plasmid resistance markers was then immediate. Ultraviolet irradiation experiments indicated that this large increase was due to release by the glycerol of double-stranded plasmid molecules, presumably from transformasomes. Several other plasmids exhibited the same phenomenon. Dimethylsulfoxide also stimulated plasmid transformation but lysolecithin and high concentrations of NaCl or glucose were ineffective. Glycerol did not increase the efficiency of transformation by either chromosomal DNA or linearized plasmid DNA. 相似文献
108.
The influence of subterranean termites on the hydrological characteristics of a Chihuahuan desert ecosystem 总被引:4,自引:0,他引:4
Summary Rainfall simulation at an average intensity of 124 mm·h-1 was used to compare infiltration and run off on arid areas where subterranean termites had been eliminated four years prior to the initiation of the study (termite free) with adjacent areas populated by subterranean termites (termites present). Infiltration rates on termite free plots with less than 5% perennial plant cover were significantly lower 51.3±6.8 mm·h-1 than rates on comparable termites present plots 88.4±5.6 mm·h-1. On plots centered on Larrea tridentata shrubs, there were no differences in infiltration rates with or without termites. Plots with shrub cover had the highest infiltration rates 101±6 mm·h-1. Highest run-off volumes were recorded from termite free <5% grass cover plots and the lowest from plots with shrubs. There were no differences in suspended sediment concentrations from termites present and termite free plots. Average bed load concentration was more than three times greater from termite free, <5% cover plots than from termites present, <5% cover plots.The reduction in infiltration, high run-off volumes and high bedloads from termite free areas without shrub cover is related to increased soil bulk density resulting from the collapse of subterranean galleries of the termites that provide avenues of bulk flow into the soil. Subterranean termites affect the hydrology of Chihuahuan desert systems by enhancing water infiltration and retention of top soil. The presence of a shrub canopy and litter layer cancels any effect of subterranean termites on hydrological parameters. Since approximately 2/3 of the area is not under shrub canopies, subterranean termites are considered to be essential for the maintenance of the soil water characteristics that support the present vegetation. 相似文献
109.
Soil respiration of Alaskan tundra at elevated atmospheric carbon dioxide concentrations 总被引:3,自引:0,他引:3
Summary CO2 efflux from tussock tundra in Alaska that had been exposed to elevated CO2 for 2.5 growing seasons was measured to assess the effect of long- and short-term CO2 enrichment on soil respiration. Long-term treatments were: 348, 514, and 683 μll−1 CO2 and 680 μll−1 CO2+4°C above ambient. Measurements were made at 5 CO2 concentrations between 87 and 680 μll−1 CO2. Neither long- or short-term CO2 enrichment significantly affected soil CO2 efflux. Tundra developed at elevated temperature and 680 μll−1 CO2 had slightly higher, but not statistically different, mean respiration rates compared to untreated tundra and to tundra under
CO2 control alone. 相似文献
110.
William D. Meek Walter L. Davis 《In vitro cellular & developmental biology. Plant》1986,22(12):725-737
Summary The potent fungal metabolite cytochalasin D (CD) and cationized ferritin (CF) are used in combination to test for negative
charge distribution on blebs (knobs). Two established human epithelial cell lines, WISH and HeLa, that display blebs in various
phases of the cell cycle or under certain culture conditions (37,46) are investigated. CD alone, applied at a low concentration
(1.0 μg/ml) and for a short time period (3 min), causes blebs to appear as the prevalent surface feature. These are filled
mainly with free ribosomes. Additionally, feltlike mats, presumed to be disorganized, compacted microfilaments, are formed
directly beneath the cell membrane. These are especially evident in the cortical cytoplasm below the blebs or bleb clusters.
CF (0.345 mg/ml), applied for a 5-min period after CD administration (1.0 μg/ml) for 3 min, appears along the surface of microvilli,
at the base of blebs, and in vesicles beneath the bleb clusters. In some cases, microfilaments (6 nm in diameter) are closely
related to the vesicles. CF does not preferentially bind to the apical cell membrane of blebs. Above areas of the subplasmalemmal
microfilaments, CF membrane binding is apparent, even under circumstances where the filaments are disorganized by cytochalasin
treatment. These results seem to show the following: (a) bleb membranes are different from the remainder of the cell and do
exhibit a loss of negative charge and (b) surface charge may be dependent on the presence or structural integrity of membrane-related
6-nm microfilaments.
The support of this research by a grant from the Baylor College of Dentistry and The Oklahoma College of Osteopathic Medicine
and Surgery is gratefully acknowledged. The assistance of Dr. J. H. Martin, Department of Pathology, Baylor University Medical
Center, is also greatly appreciated. 相似文献