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91.
92.
Genetic recombination is a fundamental evolutionary mechanism promoting biological adaptation. Using engineered recombinants of the small single-stranded DNA plant virus, Maize streak virus (MSV), we experimentally demonstrate that fragments of genetic material only function optimally if they reside within genomes similar to those in which they evolved. The degree of similarity necessary for optimal functionality is correlated with the complexity of intragenomic interaction networks within which genome fragments must function. There is a striking correlation between our experimental results and the types of MSV recombinants that are detectable in nature, indicating that obligatory maintenance of intragenome interaction networks strongly constrains the evolutionary value of recombination for this virus and probably for genomes in general. 相似文献
93.
A nationwide survey of chlorinated volatile organic compound (CVOC) plumes was conducted across a spectrum of sites from diverse hydrogeologic environments and contaminant release scenarios. The goal was to evaluate significant trends in the data that relate plume behavior to site variables (e.g., source strength, mean groundwater velocity, reductive dehalogenation regime) through correlation and population analyses. Data from 65 sites (government facilities, dry cleaners, landfills) were analyzed, yielding 247 individual CVOC plumes by compound. Data analyses revealed several trends, notably correlations between plume length and maximum observed concentration (presumably reflecting the source term) and mean groundwater velocities. Reductive dehalogenation, indicated by daughter products and groundwater geochemistry, appears to exert a relatively subtle effect on plume length, apparent only after the contributions of source strength and groundwater velocity are factored out. CVOC properties (Koc, Henry's Law constant) exert significant effects on variability in maximum observed concentrations between sites but hold little influence on plume length. Probabilistic plume modeling, entailing Monte Carlo simulation of an analytical solution for average plume behavior with parameter distributions derived from site data, was used to produce a synthetic plume set for comparison with field data. Modeling results exhibited good agreement with field data in terms of parameter sensitivity. 相似文献
94.
Investigation of nucleolus organizer regions (NORs) in hematopoietic malignancies has indicated that the distribution and rearrangement of these regions may be more important in malignant tissues than is their number. In one of the few studies thus far reported on NORs in human solid tumors, we describe here Ag-NORs in a group of human testicular germ-cell tumors and the corresponding patients. Four of seven malignancies demonstrated consistent ectopic NORs; explanations could include chromosomal rearrangement (insertion?) or derepression of preexisting inactive NORs. 相似文献
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This communication describes an inexpensive system that will monitor protein and peptide concentration in chromatogram eluates by light absorbance at an adjustable wavelength.Proteins in chromatogram eluate streams are commonly metered for concentration by absorbance measurement at 280 nm. Besides a range of commercially manufactured monitors, there is the apparatus described by Bennett et al. (1) which uses a selectively modulated magnesium lamp. Measurements in the region of 280 nm are of no value, however, when the material does not contain aromatic amino acids. Monitoring then becomes necessary at 230 nm in the region of absorption due to the peptide bond. The common resort in such a case is the standard ultraviolet-visible spectrophotometer, which has the disadvantage of being both unnecessarily elaborate and expensive for the purpose required. The deuterium lamps in these instruments require frequent replacement because of the extended periods of operation, adding to the cost factor.We have investigated the use of a hollow-cathode lamp of the type manufactured for use in an atomic absorption spectrophotometer. These lamps have high stability and a long working life, due to the considerably lower level of power dissipation compared with deuterium lamps. Their emission spectra are discontinuous, but the lamp for the element iron provides adequately strong lines at both 229.5 and 279.2 nm, suitable for a protein monitor. 相似文献
97.
The structure of the longitudinal zebra stripes on the thorax of adult Zaprionus vittiger has been investigated by light-, polarization-, transmission electron-, and scanning electron microscopy. Each stripe consists of a central white stripe of about 50 μm width and two lateral dark brown stripes about 30 μm wide. Three different types of trichomes occur: Very long bent trichomes of the grooved-type, long bent trichomes of the crested-type, and short straight trichomes. The central white stripe contains neither bristle organs nor short straight trichomes but carries many long bent trichomes most of which are of the grooved type, contain two cavities and polarize the light in the polarization microscope. The dark brown stripes carry bristle organs and many trichomes of the short and straight-type. Bent trichomes of the crested-type are found on the whole zebra stripe at about equal frequencies; they contain no cavities and do not polarize the light. The cuticle of the dark stripes is underlain by pigment cells. It is suggested that the pigment granules in the epidermal cells cause the dark color of the dark brown stripes, whereas the form and structure of the bent grooved type trichomes cause the white color of the central stripe. 相似文献
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Louise Morin Don R. Gomez Katherine J. Evans Tara M. Neill Walt F. Mahaffee Celeste C. Linde 《Biological invasions》2013,15(8):1847-1861
Field surveys in 2006 confirmed that the exotic rust fungus Phragmidium violaceum was widespread on Rubus armeniacus and Rubus laciniatus in the Pacific Northwest of the USA. The origin and dispersal pattern of this obligate biotrophic pathogen in the USA were investigated by comparing the genetic diversity and structure of 27 isolates each from the USA and Europe, and 20 isolates from Australia where an invasion occurred in 1984. Analysis of 11 microsatellite loci revealed 74 unique genotypes, with the European population having a significantly higher level of allelic diversity and number of private alleles compared to populations from the USA and Australia. Principal coordinate analysis (PCA), analysis of molecular variance and pairwise comparisons of Φ confirmed a strong level of differentiation among continental populations, with little divergence between isolates from the USA and Europe, but a high level of differentiation between these isolates and those from Australia. These results were broadly supported by the Bayesian cluster analysis, which indicated that at K = 3 the clustering of the isolates corresponds to their geographic origin. Bayesian clustering, PCA as well as insignificant migration estimates from Europe to the USA suggest that the USA population is not a direct descendant from the European P. violaceum population. There was a weak association between genetic and geographic distance among the USA isolates, suggesting invasion was initially localized prior to dispersal or that the population may have been present for some time prior to first detection in 2005. 相似文献
100.
Víctor Resco de Dios Michael L. Goulden Kiona Ogle Andrew D. Richardson David Y. Hollinger Eric A. Davidson Josu G. Alday Greg A. Barron‐Gafford Arnaud Carrara Andrew S. Kowalski Walt C. Oechel Borja R. Reverter Russell L. Scott Ruth K. Varner Rubén Díaz‐Sierra José M. Moreno 《Global Change Biology》2012,18(6):1956-1970
It is often assumed that daytime patterns of ecosystem carbon assimilation are mostly driven by direct physiological responses to exogenous environmental cues. Under limited environmental variability, little variation in carbon assimilation should thus be expected unless endogenous plant controls on carbon assimilation, which regulate photosynthesis in time, are active. We evaluated this assumption with eddy flux data, and we selected periods when net ecosystem exchange (NEE) was decoupled from environmental variability in seven sites from highly contrasting biomes across a 74° latitudinal gradient over a total of 36 site‐years. Under relatively constant conditions of light, temperature, and other environmental factors, significant diurnal NEE oscillations were observed at six sites, where daily NEE variation was between 20% and 90% of that under variable environmental conditions. These results are consistent with fluctuations driven by the circadian clock and other endogenous processes. Our results open a promising avenue of research for a more complete understanding of ecosystem fluxes that integrates from cellular to ecosystem processes. 相似文献