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排序方式: 共有218条查询结果,搜索用时 31 毫秒
61.
Miguel Hermoso de Mendoza Javier Hermoso de Mendoza Juan M. Alonso Joaquin M. Rey Sergio Sanchez Remigio Martin Felix Bermejo Maria Cortes Jose M. Benitez Waldo L. Garcia Alfredo Garcia-Sanchez 《Revista iberoamericana de micología》2010,27(2):62-65
BackgroundCats are frequent carriers of Microsporum canis and veterinary students are at high risk of exposure and acquisition of the organism a la infección.ObjectivesAn outbreak of zoonotic ringworm carried by a litter of stray cats is described. Four veterinary students, four dogs, and six cats living in five separate locations were affected. All had direct or indirect contact with the infected kitten litter. We tried to identify the causal dermatophyte.MethodsConventional and mycological culture methods were used.ResultsMicroscopic features of scrapings and hairs treated with 20% KOH strongly suggested a M. canis etiology, and a diagnosis of ringworm was empirically supported by successful treatment of humans and animals. Nevertheless, cultures failed to show the expected morphology.ConclusionsCulture features of our strain are compared with those described by other authors for dysgonic M. canis strains. Epidemiological features are also discussed. 相似文献
62.
Carolina Mascayano Gabriel Núñez Waldo Acevedo Marcos Caroli Rezende 《Journal of molecular modeling》2010,16(5):1039-1045
The lipoxygenases (LOX) are a family of non-heme iron-containing dioxygenases which catalyze the stereospecific insertion
of molecular oxygen into arachidonic acid, leading to hydroxy derivatives as end products. In this work, we docked arachidonic
acid and two of its competitive inhibitors, flavonoids baicalein and quercetin, into the binding pockets of human 12- and
15-lipoxygenase. Steered molecular dynamics (SMD) simulations were employed to study the unbinding processes of the substrate
and inhibitors from the two isoforms. 相似文献
63.
Ultrastructural evidences of HCV infection in hepatocytes of chronically HCV-infected patients 总被引:3,自引:0,他引:3
Falcón V Acosta-Rivero N Chinea G Gavilondo J de la Rosa MC Menéndez I Dueñas-Carrera S Viña A García W Gra B Noa M Reytor E Barceló MT Alvarez F Morales-Grillo J 《Biochemical and biophysical research communications》2003,305(4):1085-1090
In this study, 13 samples of liver biopsies from patients with chronic hepatitis C were studied by transmission electron microscopy (EM) and immunoelectron microscopy (IEM). The 13 biopsies showed ultrastructural cell damage typical of acute viral hepatitis. In four of the 13 liver biopsies enveloped virus-like particles (VLPs) inside cytoplasmic vesicles and in the cytoplasm of hepatocytes were observed. We also detected the presence of unenveloped VLPs mainly in the cytoplasm and in the endoplasmic reticulum. IEM using anti-core, E1 and E2 monoclonal antibodies (mAbs) confirmed the specific localization of these proteins, in vivo, inside cytoplasm and endoplasmic reticulum. Thus, this work provided evidence for hepatocellular injury related to HCV infection. It also suggested the presence of HCV-related replicating structures in the cytoplasm of hepatocytes and raised the possibility of hepatitis C virion morphogenesis in intracellular vesicles. 相似文献
64.
T H Rosenquist C Fray-Gavalas K Waldo A C Beall 《The American journal of anatomy》1990,189(4):339-356
65.
Summary Under the experimental conditions of this study no transfer of radiophosphorus occurred from one living plasmodium to another when a radioactive plasmodium ofPhysarum polycephalum and a non-radioactive plasmodium ofPhysarum gyrosum orFuligo septica were in intimate contact for 24 hours.This research constitutes a part of a program Experimental Approach to the Taxonomy of the Myxomycetes, supported by National Science Foundation Grant G-6382. 相似文献
66.
Is there a role for copper in neurodegenerative diseases? 总被引:2,自引:0,他引:2
Cerpa W Varela-Nallar L Reyes AE Minniti AN Inestrosa NC 《Molecular aspects of medicine》2005,26(4-5):405-420
Copper is an essential metal in living organisms; thus, the maintenance of adequate copper levels is of vital importance and is highly regulated. Dysfunction of copper metabolism leading to its excess or deficiency results in severe ailments. Two examples of illnesses related to alterations in copper metabolism are Menkes and Wilson diseases. Several proteins are involved in the maintenance of copper homeostasis, including copper transporters and metal chaperones. In the last several years, the beta-amyloid-precursor protein (beta-APP) and the prion protein (PrP(C)), which are related to the neurodegenerative disorders Alzheimer and prion diseases respectively, have been associated with copper metabolism. Both proteins bind copper through copper-binding domains that also have been shown to reduce copper in vitro. Moreover, this ability to reduce copper is associated with a neuroprotective effect exerted by the copper-binding domain of both proteins against copper in vivo. In addition to a functional link between copper and beta-APP or PrP(C), evidence suggests that copper has a role in Alzheimer and prion diseases. Here, we review the evidence that supports both, the role of beta-APP and PrP(C), in copper metabolism and the putative role of copper in neurodegenerative diseases. 相似文献
67.
Identification and complementation of a mutation associated with loss of Mycoplasma pneumoniae virulence-specific proteins B and C 下载免费PDF全文
A mutation in gene MPN142 (orf6) was identified in the Mycoplasma pneumoniae cytadherence mutant III-4. MPN142 encodes virulence-specific proteins P90 and P40 (proteins B and C, respectively). Analysis of MPN142 in a cytadhering revertant and complementation using a recombinant wild-type allele confirmed the role of this mutation in the cytadherence defect. 相似文献
68.
The main proteins associated with Alzheimer's and prion diseases (amyloid precursor protein (APP) and prion protein (PrP(C)), respectively, have binding sites for copper and it has therefore been suggested that they play a role in copper metabolism. Here, we review evidence indicating that the copper binding domains (CuBD) of APP and PrP(C) are able to modulate the oxidation state of copper, and prevent neurotoxic effects and memory impairments induced by copper. Results with transgenic and other animal models have established the relation between these pathogenic proteins and copper. In particular, APP transgenic models, suggest a beneficial effect for copper in AD. 相似文献
69.
Matsuo K Uno K Khrestian CM Waldo AL 《American journal of physiology. Heart and circulatory physiology》2001,280(4):H1683-H1691
A line of block between the vena cava and the crista terminalis (CT) region is important for atrial flutter (AFL), but whether it is fixed or functional is controversial. To test the hypothesis that conduction across the CT normally occurs, but when block occurs in this region it is functional, we analyzed atrial activation during right and left atrial pacing (cycle lengths of 500--130 ms), AFL, and atrial fibrillation in 15 dogs with sterile pericarditis and 7 normal dogs. Electrograms from 396 right, left, and septal atrial sites were simultaneously recorded. Activation across the CT occurred during atrial pacing, AFL, and atrial fibrillation. Activation wave fronts from the right to the left atrium and vice versa traveled over several routes, including Bachmann's bundle and inferior to the inferior vena cava, as well as across the CT. In these models, there is no fixed conduction block across the CT, and when block in the CT region occurs, as during AFL, it is functional. 相似文献
70.
Cunningham ML Waldo GL Hollinger S Hepler JR Harden TK 《The Journal of biological chemistry》2001,276(8):5438-5444
RGS proteins (regulators of G protein signaling) attenuate heterotrimeric G protein signaling by functioning as both GTPase-activating proteins (GAPs) and inhibitors of G protein/effector interaction. RGS2 has been shown to regulate Galpha(q)-mediated inositol lipid signaling. Although purified RGS2 blocks PLC-beta activation by the nonhydrolyzable GTP analog guanosine 5'-O-thiophosphate (GTPgammaS), its capacity to regulate inositol lipid signaling under conditions where GTPase-promoted hydrolysis of GTP is operative has not been fully explored. Utilizing the turkey erythrocyte membrane model of inositol lipid signaling, we investigated regulation by RGS2 of both GTP and GTPgammaS-stimulated Galpha(11) signaling. Different inhibitory potencies of RGS2 were observed under conditions assessing its activity as a GAP versus as an effector antagonist; i.e. RGS2 was a 10-20-fold more potent inhibitor of aluminum fluoride and GTP-stimulated PLC-betat activity than of GTPgammaS-promoted PLC-betat activity. We also examined whether RGS2 was regulated by downstream components of the inositol lipid signaling pathway. RGS2 was phosphorylated by PKC in vitro to a stoichiometry of approximately unity by both a mixture of PKC isozymes and individual calcium and phospholipid-dependent PKC isoforms. Moreover, RGS2 was phosphorylated in intact COS7 cells in response to PKC activation by 4beta-phorbol 12beta-myristate 13alpha-acetate and, to a lesser extent, by the P2Y(2) receptor agonist UTP. In vitro phosphorylation of RGS2 by PKC decreased its capacity to attenuate both GTP and GTPgammaS-stimulated PLC-betat activation, with the extent of attenuation correlating with the level of RGS2 phosphorylation. A phosphorylation-dependent inhibition of RGS2 GAP activity was also observed in proteoliposomes reconstituted with purified P2Y(1) receptor and Galpha(q)betagamma. These results identify for the first time a phosphorylation-induced change in the activity of an RGS protein and suggest a mechanism for potentiation of inositol lipid signaling by PKC. 相似文献