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131.
132.
S. Waite 《Journal of Ecology》2000,88(5):935-936
133.
Age‐specific vibrissae growth rates: A tool for determining the timing of ecologically important events in Steller sea lions
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Lorrie D. Rea Aaron M. Christ Alison B. Hayden Vicki K. Stegall Sean D. Farley Craig A. Stricker Jo‐Ann E. Mellish John M. Maniscalco Jason N. Waite Vladimir N. Burkanov Kenneth W. Pitcher 《Marine Mammal Science》2015,31(3):1213-1233
Steller sea lions (SSL; Eumetopias jubatus) grow their vibrissae continually, providing a multiyear record suitable for ecological and physiological studies based on stable isotopes. An accurate age‐specific vibrissae growth rate is essential for registering a chronology along the length of the record, and for interpreting the timing of ecologically important events. We utilized four methods to estimate the growth rate of vibrissae in fetal, rookery pup, young‐of‐the‐year (YOY), yearling, subadult, and adult SSL. The majority of vibrissae were collected from SSL live‐captured in Alaska and Russia between 2000 and 2013 (n = 1,115), however, vibrissae were also collected from six adult SSL found dead on haul‐outs and rookeries during field excursions to increase the sample size of this underrepresented age group. Growth rates of vibrissae were generally slower in adult (0.44 ± 0.15 cm/mo) and subadult (0.61 ± 0.10 cm/mo) SSL than in YOY (0.87 ± 0.28 cm/mo) and fetal (0.73 ± 0.05 cm/mo) animals, but there was high individual variability in these growth rates within each age group. Some variability in vibrissae growth rates was attributed to the somatic growth rate of YOY sea lions between capture events (P = 0.014, r2 = 0.206, n = 29). 相似文献
134.
We describe a method to determine the species of pinniped from faeces collected from sympatric Steller sea lion (Eumetopias jubatus) and northern fur seal (Callorhinus ursinus) rookeries using newly developed species-specific primers that amplify a 667-669-base pair segment from the mitochondrial DNA (mtDNA) cytochrome B (cytB) gene region. The primers yielded the correct species in 100% of tissue samples from 10 known animals and 100% of faecal samples from 13 known animals. Species could be identified unequivocally for 87.7% of faecal samples from 122 unknown individuals. The ability to differentiate between scats of sympatrically breeding Steller sea lions and northern fur seals will contribute to the range-wide knowledge of the foraging strategies of both species as well as allow researchers to examine the niche partitioning and potential resource competition between the two predators. 相似文献
135.
The mechanisms governing the efficient tumor spheroid penetration and transport by poly(amidoamine) (PAMAM) dendrimers displaying varying numbers of cyclic RGD targeting peptides (2, 3, 7, or 10) were evaluated in this work. The cell-free binding affinities and cellular internalization kinetics of PAMAM-RGD conjugates to malignant glioma cells were determined experimentally, and the results were incorporated into a mathematical model to predict the transport of these materials through a multicellular tumor spheroid. The theoretical analysis demonstrated that greater RGD crosslinking may improve transport through tumor spheroids due to their decreased integrin-binding affinity. This study provides evidence that altering the density of tumor-targeting ligands from a drug delivery platform is a feasible way to optimize the tumor-penetration efficiency of an anticancer agent, and provides insight into the physicochemical mechanisms governing the relative effectiveness of these conjugates. 相似文献
136.
R. F. Mark D. L. Flett L. R. Marotte P. M. E. Waite 《Somatosensory & motor research》2013,30(3):198-206
This study used the extrauterine development of a marsupial wallaby to investigate the onset of functional activity in the somatosensory pathway from the whiskers. In vivo recordings were made from the somatosensory cortex from postnatal day (P) 55 to P138, in response to electrical stimulation of the infraorbital nerve supplying the mystacial whiskers. Current source density analysis was used to localize the responses within the cortical depth. This was correlated with development of cortical lamination and the onset of whisker-related patches, as revealed by cytochrome oxidase. The earliest evoked activity occurred at P61, when layers 5 and 6 are present, but layer 4 has not yet developed. This activity showed no polarity reversal with depth, suggesting activity in thalamocortical afferents. By P72 synaptic responses were detected in developing layer 4 and cytochrome oxidase showed the first hint of segregation into whisker-related patches. These patches were clear by P86. The evoked response at this age showed synaptic activity first in layer 4 and then in deep layer 5/upper layer 6. With maturity, responses became longer lasting with a complex sequence of synaptic activity at different cortical depths. The onset of functional activity is coincident with development of layer 4 and the onset of whisker-related pattern formation. A similar coincidence is seen in the rat, despite the markedly different chronological timetable, suggesting similar developmental mechanisms may operate in both species. 相似文献
137.
High-spired nerineoid gastropods are abundant in the tropical Kimmeridgian carbonate deposits of the Swiss Jura Mountains.
Understanding the mode of life of this extinct group of gastropods is crucial for palaeoenvironmental reconstructions. The
knowledge on their ecology is, however, limited by the near absence of comparable large high-spired gastropods in Recent carbonate
systems. Large high-spired turritellid gastropods are, however, abundant in siliciclastic-dominated environments in the tropical
Indian Ocean. In order to compare the Recent and fossil environments of these two morphologically similar groups, a Recent
tidal flat, where abundant turritellids occur, was studied in the National Park of Nopparat Thara in the district of Krabi,
South Thailand. Ninety specimens of Turritella duplicata were measured and mapped, revealing zonations in population distribution, both parallel and perpendicular to the beach line.
Attention was also paid to other faunal elements and sedimentary features. The thus-gained information on the depositional
environment was then compared to a section of shallow-water carbonate deposits from the Kimmeridgian of the Swiss Jura Mountains.
These are characterised by the occurrence of tidal laminites, dinosaur tracks, beach deposits and nerineoid gastropods. Especially
for one succession, the faunal composition and sedimentary structures in the fossil record show intriguing similarities to
those found today on the Nopparat Thara tidal flat, indicating that it formed in a comparable setting. It is therefore suggested
that for some Kimmeridgian carbonate deposits, the Nopparat Thara tidal flat, although dominantly siliciclastic, represents
an environmental analogue. 相似文献
138.
We examined the importance of picoplankton and virioplankton to reef trophodynamics at Ningaloo Reef, (north-western Australia),
in May and November 2008. Picophytoplankton (Prochlorococcus, Synechococcus and picoeukaryotes), bacterioplankton (inclusive of bacteria and Archaea), virioplankton and chlorophyll a (Chl a) were measured at five stations following the consistent wave-driven unidirectional mean flow path of seawater across the
reef and into the lagoon. Prochlorococcus, Synechococcus, picoeukaryotes and bacterioplankton were depleted to similar levels (~40% on average) over the fore reef, reef crest and
reef flat (=‘active reef’), with negligible uptake occurring over the sandy bottom lagoon. Depletion of virioplankton also
occurred but to more variable levels. Highest uptake rates, m, of picoplankton occurred over the reef crest, while uptake coefficients, S (independent of cell concentration), were similarly scaled over the reef zones, indicating no preferential uptake of any
one group. Collectively, picophytoplankton, bacterioplankton and virioplankton accounted for the uptake of 29 mmol C m−2 day−1, with Synechococcus contributing the highest proportion of the removed C. Picoplankton and virioplankton accounted for 1–5 mmol N m−2 day−1 of the removed N, with bacterioplankton estimated to be a highly rich source of N. Results indicate the importance of ocean–reef
interactions and the dependence of certain reef organisms on picoplanktonic supply for reef-level biogeochemistry processes. 相似文献
139.
Qing-Long Fu Anna C. Y. Yeung Manabu Fujii Brett A. Neilan T. David Waite 《Environmental microbiology》2019,21(4):1211-1223
The cyanobacterium Raphidiopsis raciborskii is of environmental and social concern in view of its toxicity, bloom-forming characteristics and increasingly widespread occurrence. However, while availability of macronutrients and micronutrients such as N and Fe are critically important for the growth and metabolism of this organism, the physiological response of toxic and non-toxic strains of R. raciborskii to varying Fe and N availabilities remains unclear. By determining physiological parameters as a function of Fe and N availability, we demonstrate that R. raciborskii growth and N2-fixing activity are facilitated at higher Fe availability under N2-limited conditions with faster growth of the CS-506 (cylindrospermopsin-producing) strain compared with that of CS-509 (the non-toxic) strain. Radiolabelled Fe uptake assays indicated that R. raciborskii acclimated under Fe-limited conditions acquires Fe at significantly higher rates than under Fe replete conditions, principally via unchelated Fe(II) generated as a result of photoreduction of complexed Fe(III). While N2-fixation of both strains occurred during both day and night, the CS-506 strain overall exhibited higher N2-fixing and Fe uptake rates than the CS-509 strain under N-deficient and Fe-limited conditions. The findings of this study highlight that Fe availability is of significance for the ecological advantage of CS-506 over CS-509 in N-deficient freshwaters. 相似文献
140.
Ian R. Waite 《Hydrobiologia》2014,726(1):285-303
As part of the USGS study of nutrient enrichment of streams in agricultural regions throughout the United States, about 30 sites within each of eight study areas were selected to capture a gradient of nutrient conditions. The objective was to develop watershed disturbance predictive models for macroinvertebrate and algal metrics at national and three regional landscape scales to obtain a better understanding of important explanatory variables. Explanatory variables in models were generated from landscape data, habitat, and chemistry. Instream nutrient concentration and variables assessing the amount of disturbance to the riparian zone (e.g., percent row crops or percent agriculture) were selected as most important explanatory variable in almost all boosted regression tree models regardless of landscape scale or assemblage. Frequently, TN and TP concentration and riparian agricultural land use variables showed a threshold type response at relatively low values to biotic metrics modeled. Some measure of habitat condition was also commonly selected in the final invertebrate models, though the variable(s) varied across regions. Results suggest national models tended to account for more general landscape/climate differences, while regional models incorporated both broad landscape scale and more specific local-scale variables. 相似文献