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111.
PSⅡ反应中心D1蛋白的小肽抗体的制备和鉴定 总被引:7,自引:3,他引:4
用人工合成的PSⅡ反应中心D1蛋白中(229~240)的12肽,与牛血清白蛋白偶联后做为抗原免疫家兔, 获得抗菠菜D1蛋白抗体. 免疫双扩散法测得其具较高的效价,蛋白印迹检测结果显示该抗体仅与D1蛋白有免疫亲和反应. 表明此抗体可作为检测D1蛋白及其光抑制时降解产物的探针. 相似文献
112.
以双引物法对葡萄糖异构酶(GI)基因进行定点突变,将突变体基因于大肠杆菌中表达,获得了GI双点突变体GIK253RA198C.研究K253R和A198C双点突变对GI的结构和性质的作用,结果表明GIK253RA198C的热稳定性明显下降,最适反应温度降低5℃.文章从结构和机制上解释了为何同是K253R突变,对SM33 GI和密苏里游动放线菌GI的热稳定性产生不同的影响,认为这是由于Lys253在两种GI结构的位置上存在微小差异,从而使引入的Arg对亚基间的相互作用产生了相反效应所引起. 相似文献
113.
To reveal how the matching models of the left ventricle and its afterload affect the pressure and flow in the aortic root, the differences between the measured pressure and flow waveforms and those determined by three kinds of matching model were compared. The results showed that, compared with the results by both matching models 1 and 2, the pressure and flow waveforms determined by matching model 3 established in this work were in the closest agreement with the corresponding experimental waveforms, therefore indicating that matching model 3 was a matching model that closely and rationally characterized the match between the left ventricle and the systemic artery. 相似文献
114.
YANG Jianbo WU Lijun LI Li WU JiadaoYU Zengliang XU Zhihong 《中国科学:生命科学英文版》1997,40(1):107-112
While M13mp18 double-stranded DNA was irradiated with ion beam, and transfected intoE. coli JM103, a decrease of transfecting activity was discovered. The lacZ- mutation frequency at 20% survival could reach (3.6–16.8) × 104, about 2, 3–10 times that of unirradiated M13DNA. Altogether, 27 IacZ-mutants were selected, 10 of which were used for sequencing. 7 of the sequenced mutants show base changes in 250-bp region
examined (the remaining 3 mutants probably have base changes outside the regions sequenced). 5 of the base-changed mutants
contain more than one mutational base sites (some of them even have 5–6 mutational base sites in 250-bp region examined);
this dense distribution of base changes in polysites has seldom been seen in X-rays, Y-rays or UV induced DNA mutations. Our
experiments also showed that the types of base changes include transitions(50%), transversions (45%) and deletion (5%); no
addition or duplication was observed. The transitions were mainly C→T and A→G; the transversions were mainly C→A and C→G.
The mutations involving cytosine residue (in the template strand) constitute about 60% of all the base changes observed. In
comparison with the surrounding sequences of mutational base sites, the base located between TG and CT is found to be easily
substituted. 相似文献
115.
A temperature-sensitive (ts) mutant of the influenza virus A/WSN/ 33 strain, ts-134, possessed a defect in intracellular transport at the nonpermissive temperature and marked thermolability of hemagglutinin (HA) activity at 51 C. These were caused by a change at amino acid residue 157 from tyrosine to histidine in the HA protein. We isolated 37 spontaneous revertant clones from ts-134 at the nonpermissive temperature and determined their HA sequences. The deduced amino acid sequences demonstrated that one was a true revertant and the others were revertants with suppressor mutations, each of which had an additional amino acid change besides those of ts-134. The changed amino acids were located at 14 positions on the HA molecule, and eight of them were found in multiple revertants. These were located in five to six distinct regions on the three-dimensional structure of the HA molecule. However, the heat stability of HAs in the revertants was recovered differently depending on the sites of the changed amino acids. The kinetics of transport of the HA protein in the revertants were slightly delayed compared to the wild-type both at permissive and nonpermissive temperatures. 相似文献
116.
囊萼紫草属与滇紫草属花粉形态比较研究 总被引:3,自引:1,他引:2
本文借助光学显微镜和扫描电镜研究了囊萼紫草属3种和滇紫草属12种植物的花粉形态。囊萼紫草属的花粉为哑铃形或茧形,中等大小,P/E比为1.6一1.67,三孔沟,内孔横长;具小刺状纹饰。滇紫草属的花粉为近长球形或近卵球形,P/E为l—1.23;三孔沟或三合沟孔,内孔一般纵长,具皱波状纹饰,在皱波上具密集的小瘤或微颗粒。从花粉形态的角度,本文支持把囊萼紫草属从滇紫草属(广义)中分离出来的观点。值得注意的是,在滇紫草属的花粉中首次观察到了一种比较少见且特化的花粉即单极三合沟孔的花粉。 相似文献
117.
Narendra Tuteja Ning Wu Huang Doris Skopac Renu Tuteja Sara Hrvatic Jianwen Zhang Sandor Pongor Grard Joseph Christian Faucher Franois Amalric Arturo Falaschi 《Gene》1995,160(2):143-148
The cDNA encoding human DNA helicase IV (HDH IV), a 100-kDa protein which unwinds DNA in the 5′ to 3′ direction with respect to the bound strand, was cloned and sequenced. It was found to be identical to the human cDNA encoding nucleolin, a ubiquitous eukaryotic protein essential for pre-ribosome assembly. HDH IV/nucleolin can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes. Phosphorylation of HDH IV/nucleolin by cdc2 kinase and casein kinase II enhanced its unwinding activity in an additive way. The Gly-rich C-terminal domain possesses a limited ATP-dependent duplex-unwinding activity which contributes to the helicase activity of HDH IV/nucleolin. 相似文献
118.
119.
昆虫神经毒素的研究:各种神经毒剂引起毒素的产生 总被引:2,自引:1,他引:1
用五类(七种)神经毒性的杀虫药剂,对美洲(虫非)蠊测定了它们能否引起血淋巴中毒素的产生。DDT、溴氰菊酯及六六六均能引起血淋巴中毒素的产生,而E605、西维因、巴丹及杀虫脒均无此效果。纸层析及薄层层析,用标准样品(酪氨酸、酪胺、苯乙胺、L-亮氨酸及异戊胺)作比较,测定了这一产生的血淋巴中的毒素乃是酪胺,或主要是酪胺。增效试验,证明了杀虫脒与DDT或溴氰菊酯合用时,能增加酪胺的产量。讨论了这一毒素产生的条件,以及这一毒素有可能不是单一成分,而是几种单胺及氨基酸的复合物,但酪胺为其主要成分。 相似文献
120.