An Audit of Cervical Cancer Deaths In Nottingham

Death certificates were reviewed and 57 women were identified whose primary cause of death was cervical cancer. Their cervical smear records were reviewed from laboratory files. Only nine had participated in the cervical cancer screening programme before the diagnosis of cervical cancer was made. One woman had an abnormal smear as a result of cervical screening but failed to attend for follow-up.
The problem of false negative smears is also addressed and the value of review of negative smears for the purposes of cytology audit is emphasized.     

Measuring the Canopy Net Photosynthesis of Glasshouse Crops

A null balance method is described for measuring net photosynthesisof mature canopies of cucumber and other protected crops overperiods of 10 min in a single-span glasshouse (c. 9m x 18m inarea). Accuracy of control of the CO₂ concentration in the greenhouseatmosphere is within ±10 vpm of the normal ambient level(c. 350 vpm). The amounts of CO₂ used in canopy net photosynthesisare measured with linear mass flowmeters accurate to within±0.80g. The total errors incurred in measuring canopynet photosynthesis at an ambient CO₂ level are estimated tobe of the order of ± 1·2% in bright light (350W m^–2, PAR)and ±3·6% in dull light (100W m^–2, PAR). Measurements of the rates of net photosynthesis of a maturecanopy of a cucumber crop were made at near-ambient CO₂ concentrationsover a range (0–350 W m^–2) of natural light fluxdensities. A model of light absorption and photosynthesis applicableto row crops was used to obtain a net photosynthesis versuslight response curve for the cucumber crop. At a light fluxdensity of 350 W m^–2 the fitted value of canopy net photosynthesiswas 2.65 mg CO₂ m^–2s^–1 (equivalent to over 95 kgCO₂ ha^–1h^–1). The results are discussed in relationto the need for CO₂ supplements to avoid depletion in both ventilatedand unventilated glasshouses during late spring and summer. Key words: Glasshouse crops, cucumber, measurement, canopy photosynthesis, light, CO₂     

A NEW GENUS OF ARCHIPOLYPODAN MILLIPEDE FROM THE COSELEY LAGERSTÄTTE, UPPER CARBONIFEROUS, UK

Abstract:  A new archipolypodan millipede, Anaxeodesmus diambonotus gen. et sp. nov., is described from the Upper Carboniferous (Westphalian B) Coseley Lagerstätte, West Midlands, UK. This taxon is unique amongst Archipolypoda in having tergites unornamented by spines or tubercles. It appears to have archidesmidan affinities as it shares similarities in sternal morphology with the Early Devonian archipolypodan Palaeodesmus tuberculata (Brade-Birks) from the Midland Valley of Scotland. Anaxeodesmus diambonotus significantly increases the ordinal diversity of Archipolypoda in the Late Carboniferous as all other Archipolypoda from this time period belong to the spiny Euphoberiida.     

Ultrastructural Observations on the Gametocytic Stages of the Coccidium Tyzzeria chalcides Probert, Roberts & Wilson, 1988 from the Ocellated Skink Chalcides ocellatus

Gametogenesis of Tyzzeria chalcides Probert, Roberts & Wilson, 1988, from the ocellated skink, Chalcides ocellatus , occurs within the epithelium of the gali bladder. Transmission electron microscopy reveals that macrogamonts contain 2 types of wall-forming bodies. Type I bodies are large densely stained structures associated with rough endoplasmic reticulum and the Golgi apparatus. They appear to be formed within the Golgi itself. Type II bodies are less densely stained, smaller and appear to form directly from the rough endoplasmic reticulum. Canaliculi are associated with Type I wall-forming bodies and probably function to transport the wall-forming bodies to the pellicle. Micropores occur in the pellicie and large amylopectin granules, lipid globules and dense bodies are found within the cytoplasm of the macrogamont. Mature microgamonts contain in excess of 20 microgametes, each of which has 2 flagella and an associated mitochondrion. Both types of gamont are found within a parasitophorous vacuole, in the host cell, which is filled with vesicular material on which the gamonts probably feed.     

The incidence of Listeria monocytogenes in raw milk from farm bulk tanks in North-East Scotland

Over 180 farm bulk milk tanks were tested for the presence of Listeria monocytogenes on three separate occasions which included periods when cows were grazing and confined inside on a silage diet. The incidence of L. monocytogenes contamination was low, ranging from 3.8% in the summer samples to 1.0% in October. The level of contamination was estimated to be lower than one L. monocytogenes bacterium per ml in positive samples, as most required cold enrichment of 10–20 ml volumes before recovery. The distribution was sporadic; only one farm gave positive isolations on all three sampling occasions, one other on two, and all others were from different farms. No correlation between the presence of L. monocytogenes and hygiene standards or the feeding of silage was found.     

Post-dispersal seed predation of an exotic weed,Mimosa pigra L., in the Northern Territory

The removal of seeds of the exotic shrub Mimosa pigra from the ground beneath a thicket of the plant and from the open floodplain surrounding the thicket was studied experimentally. Seeds placed beneath the canopy of the M. pigra thicket were taken predominantly by vertebrates (probably omnivorous rodents) while those on the open floodplain were taken predominantly by ants. After 14 days exposure with no barriers to seed removal, less than half of the seeds placed in either habitat had disappeared. Of the seeds that were removed, over 75% had disappeared from both habitats within 5 days. It is unlikely that post-dispersal seed predation appreciably inhibits the spread or maintenance of stands of this weed in tropical Australia.     

Chemical Composition of the Epidermal, Hypodermal, Endodermal and Intervening Cortical Cell Walls of Various Plant Roots

A survey of chemical modifications in the cells of the epidermis,hypodermis, cortical parenchyma and endodermis in roots of 27plant species was performed. Cortical parenchyma walls weregenerally free of modifying substances whereas the walls ofthe epidermis, hypodermis and endodermis were usually modifiedby the presence of lipids, phenols, suberin or lignin. In mostcases, wall-modifying components could be detected within 5mm of the root apex. lipids, phenols, suberin, lignin, ferulic acid, root, epidermis, hypodermis, cortex, endodermis, cell wall     

Stomatal diffusion resistances and leaf growth during droughting of Lolium perenne plants selected for contrasting epidermal ridging

Groups of Lolium perenne plants selected for either deep or shallow adaxial epidermal ridging were grown in a 16 h day of 70 W m^-2 at 25°C, and either watered daily to 33% or allowed to dry to and then watered daily to 21% or to 16% soil moisture. During a 9 day experimental period, adaxial leaf resistances (r₁) were measured with a diffusion porometer four times daily, transpiration was estimated gravimetrically, and daily rates of leaf extension were recorded. Measurements were also made of minimum abaxial resistances, stomatal frequencies and lengths, and relative leaf water content (RLWC). At 33%, 21% or 16% soil moisture, leaf extension rates of deep ridged leaves were, respectively, slower, the same, and more rapid than those with shallow ridges. At 21% or 16% soil moisture, the adaxial r_l of deep-ridged was much lower than that of shallow-ridged leaves at all four sampling times. This difference was most marked on leaves below the youngest fully expanded, and was observed among older leaves even when plants were well watered. At low RLWC (< 85%), leaf resistance was greatest in leaves with shallow ridges. There was no significant difference between the leaf types in the calculated contributions of stomatal frequency or of morphology at any one pore opening, to r_l but deep-ridged leaves had more stomataonthe abaxial surface. Daily rate of plant water loss was directly correlated (r=+ 0.86, P < 0.01) with mean daily maximum stomatal conductance (1/r_l), and rate of leaf extension negatively with maximum r_l. It is suggested that stomata operating in the concavity formed by deep ridges open wider and are less responsive to internal changes in, for example, leaf water status, than those on shallow-ridged leaves because of a more humid microenvironment at the epidermal surface. The results are discussed in relation to the concept of ‘water-savers’ and ‘water-spenders’ and its application to breeding for dry conditions.     

Growth Regulation of Galium mollugo L. Cell Suspensions by a-Naphthalene Acetic Acid

Fidgeon, C. and Wilson, G. 1987. Growth regulation of Galiummollugo L. cell suspensions by -naphthalene acetic acid.—J.exp. Bot. 38: 1491–1500. Galium mollugo cell suspension cultures were found to requirethe plant growth regulator -naphthalene acetic acid (-NAA) forcontinued growth and cell division. This requirement could notbe substituted in either batch or semi-continuous culture byindole-3-acetic acid (IAA) or 2,4-dichlorophenoxy acetic acid(2,4-D) at any concentration tested. However, ß-naphthaleneacetic acid (ß-NAA) and indole-3-butyric acid (IBA)were found to support growth when supplied at a concentrationtwo orders of magnitude greater than the normal media level(0–5 mg dm³). The growth of Galium cells was found to be influenced not onlyby the -NAA initially supplied in the medium but also by theexposure to -NAA in previous growth cycles. Preculture of cellsfor 3 d in an -NAA containing medium, followed by cell washingand re-inoculation into -NAA free medium, supported a quantitativegrowth response similar to that obtained after 14 d in the control-NAA containing medium. Even short-term exposures between 0·5and 6·0 h stimulated a detectable growth response 14d later. These observations raise questions relating to theuptake and perception of exogenously supplied growth regulatorsby cultured cells. The delayed kinetics of this form of response is of significancein culture regimes in which cells are transferred from one mediumto another, differing in their growth regulator composition,in order to induce morphogenesis     

Philosophical Aspects of Measurements, Equations and Inferences in Plant Growth Studies

Growth analysis is based on equations that are ‘identities’because they are algebraically self-evident, whereas the moredeterministic models of plant growth are based on ‘conditionalequations’ that represent quantitative hypotheses. Growthanalytical studies consequently focus on the values of the componentsand not on the validity of the equations, whereas ‘validation’is a prime concern for other growth models. Implications ofmeasurement theory, of dependent and independent variables andof compensating components arise in the use of both types ofequation for quantifying growth. There is now available a rangeof approaches, from traditional growth analysis, through variousdevelopments of growth analysis including light conversion analysis,to complex mechanistic models of growth. Growth analysis, yield component analysis, light conversion analysis, mathematical models, measurement theory, derived quantities, independent variables, equations of growth