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161.
Using Polyacrylamide gel electrophoresis, cycloheximide, incorporation of 3H-labelled amino acids and immunological methods, we have demonstrated that injection of ecdy- sterone induces de novo synthesis and release of vitellogenin in both sexes of Sarcophaga bullata. Vitellogenin concentrations were measured by the Mancini-radial immunodiffusion technique. In males a dose as low as 1 ng always makes vitellogenin appear in the haemolymph but very reproducible results are only obtained when doses varying from 10 to 250 ng were injected. In this range, the dose-response curve was linear on a semi- logarithmic scale. In females, vitellogenin concentration remained low until a few hours after liver feeding and thereafter it rose sharply and reached its maximum about 24 h after the protein meal. 100 μg 6-hydroxydopamine HCl, injected before liver feeding in 4-day-old females, inhibited vitellogenin synthesis and yolk deposition, probably by interfering with the release of a brain hormone. This inhibitory effect on vitellogenin synthesis, but not that on yolk deposition, could be overruled by injection of ecdysterone. Juvenile hormone was ineffective on both. Females, ovariectomized on day 2 or 3, accumulated vitellogenin in their haemolymph, indicating that the continuous presence of the ovaries was not required for vitellogenin synthesis. The possible relation between the gonadotrophs hormone from the brain, vitellogenin synthesis and moulting hormone metabolism is discussed. 相似文献
162.
A. DE LOOF ZHU WEI R. HUYBRECHTS J. GIJBELS P. VERHAERT 《Invertebrate reproduction & development.》2013,57(1-3):69-74
Summary RH5849 is a benzoyl hydrazine analog which has been reported to mimic several effects of the arthropod steroid hormone ecdysone to which it is chemically totally unrelated. In adult Diptera, ecdysone is the hormone that triggers vitellogenin synthesis. We report here that RH5849, upon oral ingestion, is able to induce vitellogenin synthesis in male Drosophila, Neobellieria, Phormia and Lucilia. This contrasts to data in the literature which showed that RH5849 could not mimic the pupariation-inducing effect of ecdysone in last instar fly larvae. RH5849 neither exerts a juvenile hormone mimicking effect nor behaves as an anti-juvenile hormone in both the Colorado potato beetle and Galleria. 相似文献
163.
ANDREA DE OLIVEIRA RIBEIRO JUNQUEIRA CARLOS RENATO REZENDE VENTURA ADRIANA LUIZA P.S. DE CARVALHO ANDERS JENSEN SCHMIDT 《Invertebrate reproduction & development.》2013,57(1-3):143-150
Summary The size, structure, and density of a population of Lytechinus variegatus were recorded from a shallow seagrass bed (Halodule wrightii) at 40-day intervals from April 1994 to April 1995. A local mass mortality was observed in May 1994. The density declined from 0.57±0.10 to 0.06±0.05 ind.m?2, mean size declined from 37.6±0.49 to 16.2±2.14 mm and maximum size from 75 to 40 mm. Ten months after mortality, the maximum and mean sizes had returned to initialvalues, but density had not (0.10±0.03 ind.m?2). A second, smaller mass mortality occurred in April 1995. High values of gonad index were recorded before mortality (9.40±5.97) and after population recovery (11.96±5.74). Recruitment was continuous throughout the year, but reached a peak from August to October. This can he related to the patterns of upwelling in the region. Mass mortality is probably an annual event in this population that can be correlated with the occurrence of low tides during daytime in the fell. The population can recover during the summer when low tides occur during the night. The massive input of recruits prevents extinction of the population during the season of high mortality. Population recovery depends on the magnitude of recruitment. 相似文献
164.
165.
The K+/H+ antiporter LeNHX2 increases salt tolerance by improving K+ homeostasis in transgenic tomato 总被引:1,自引:0,他引:1
RAÚL HUERTAS LOURDES RUBIO OLIVIER CAGNAC MARÍA JESÚS GARCÍA‐SÁNCHEZ JUAN DE DIOS ALCHÉ KEES VENEMA JOSÉ ANTONIO FERNÁNDEZ MARÍA PILAR RODRÍGUEZ‐ROSALES 《Plant, cell & environment》2013,36(12):2135-2149
The endosomal LeNHX2 ion transporter exchanges H+ with K+ and, to lesser extent, Na+. Here, we investigated the response to NaCl supply and K+ deprivation in transgenic tomato (Solanum lycopersicum L.) overexpressing LeNHX2 and show that transformed tomato plants grew better in saline conditions than untransformed controls, whereas in the absence of K+ the opposite was found. Analysis of mineral composition showed a higher K+ content in roots, shoots and xylem sap of transgenic plants and no differences in Na+ content between transgenic and untransformed plants grown either in the presence or the absence of 120 mm NaCl. Transgenic plants showed higher Na+/H+ and, above all, K+/H+ transport activity in root intracellular membrane vesicles. Under K+ limiting conditions, transgenic plants enhanced root expression of the high‐affinity K+ uptake system HAK5 compared to untransformed controls. Furthermore, tomato overexpressing LeNHX2 showed twofold higher K+ depletion rates and half cytosolic K+ activity than untransformed controls. Under NaCl stress, transgenic plants showed higher uptake velocity for K+ and lower cytosolic K+ activity than untransformed plants. These results indicate the fundamental role of K+ homeostasis in the better performance of LeNHX2 overexpressing tomato under NaCl stress. 相似文献
166.
167.
ABSTRACT The vocal repertoire of Amazona amazonica during its breeding season has been recorded from wild individuals in Santa Bárbara do Pará, Pará State, Brazil. At individual nests, we continuously recorded vocalizations and behaviour for four hours in the early morning and three hours in the late afternoon, three times a week throughout the breeding season. We identified nine vocalizations that we classified in three behavioural categories: (1) Flight call—emitted when parrots arrive in the nest area; (2) Perched contact calls—two different vocalizations, one of them related to feeding, were emitted when the pair was perched in the nest area and interacted socially between themselves or with other individuals; (3) Aggressive calls—emitted when birds were in a dangerous situation, i.e. alarm (three types of calls), agonistic contact and distress calls (two types of call). The Orange-winged Parrot is a highly social species and the complexity of its social interactions is reflected in the diversity of its vocal repertoire. 相似文献
168.
MARIA CLARA PESSOA DE AMORIM 《Bioacoustics.》2013,22(4):265-272
ABSTRACT Sound production is widespread in coral reef fish of the family Pomacentridae. As commonly found in other teleosts, damselfish emit calls in agonistic and courtship contexts. In this paper, evidence of sound production by the blue-green damselfish Chromis viridis is presented for the first time. The recorded sounds consisted of clicks produced during agonistic interactions. 相似文献
169.
170.
TARCILA DE LIMA NADIA NANUZA LUIZA DE MENEZES ISABEL CRISTINA MACHADO 《Plant Species Biology》2013,28(1):70-80
Pollination systems may vary in their degree of specialization from generalist to specialist and this is associated with the frequency and efficiency of pollinators. Self‐compatibility and a generalist pollination system appear to be adaptations that enhance the ability for colonizing new areas, which is typical of mangrove species. Avicennia schaueriana is a western mangrove species included in Acanthaceae, Lamiales, an order known to have specialized pollination. We aimed to describe the floral morphology and analyze the pollination and reproductive systems of A. schaueriana in a mangrove area on the northern coast of the state of Pernambuco, Brazil, to analyze the possible adaptations of this species to the environment. Avicennia schaueriana is self‐compatible; however, pollination activity is essential because there is no spontaneous formation of fruit. Reproductive efficacy was high, indicating pollinator efficiency. Some floral attributes of A. schaueriana suggest specialization; however, the broad spectrum of pollinators observed suggests that it has a generalist pollination system. The way in which pollinators interact with flowers and the environmental conditions may have exerted a selective force on the floral attributes of A. schaueriana, characterizing an adaptive generalized pollination system, which is somewhat specialized. 相似文献