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排序方式: 共有197条查询结果,搜索用时 31 毫秒
31.
Effects of soil frost on soil respiration and its radiocarbon signature in a Norway spruce forest soil 总被引:3,自引:0,他引:3
Apart from a general increase of mean annual air temperature, climate models predict a regional increase of the frequency and intensity of soil frost with possibly strong effects on C cycling of soils. In this study, we induced mild soil frost (up to −5 °C in a depth of 5 cm below surface) in a Norway spruce forest soil by removing the natural snow cover in the winter of 2005/2006. Soil frost lasted from January to April 2006 and was detected down to 15 cm depth. Soil frost effectively reduced soil respiration in the snow removal plots in comparison to undisturbed control plots. On an annual basis 6.2 t C ha−1 a−1 were emitted in the control plots compared with 5.1 t C ha−1 a−1 in the snow removal plots. Only 14% of this difference was attributed to reduced soil respiration during the soil frost period itself, whereas 63% of this difference originated from differences during the summer of 2006. Radiocarbon (Δ14C) signature of CO2 revealed a considerable reduction of heterotrophic respiration on the snow removal plots, only partly compensated for by a slight increase of rhizosphere respiration. Similar CO2 concentrations in the uppermost mineral horizons of both treatments indicate that differences between the treatments originated from the organic horizons. Extremely low water contents between June and October of 2006 may have inhibited the recovery of the heterotrophic organisms from the frost period, thereby enhancing the differences between the control and snow removal plots. We conclude that soil frost triggered a change in the composition of the microbial community, leading to an increased sensitivity of heterotrophic respiration to summer drought. A CO2 pulse during thawing, such as described for arable soils several times throughout the literature, with the potential to partly compensate for reduced soil respiration during soil frost, appears to be lacking for this soil. Our results from this experiment indicate that soil frost reduces C emission from forest soils, whereas mild winters may enhance C losses from forest soils. 相似文献
32.
Effects of soil frost on nitrogen net mineralization, soil solution chemistry and seepage losses in a temperate forest soil 总被引:1,自引:0,他引:1
KERSTIN HENTSCHEL WERNER BORKEN TOBIAS ZUBER CHRISTINA BOGNER† BERND HUWE† EGBERT MATZNER 《Global Change Biology》2009,15(4):825-836
Freezing and thawing may alter element turnover and solute fluxes in soils by changing physical and biological soil properties. We simulated soil frost in replicated snow removal plots in a mountainous Norway spruce stand in the Fichtelgebirge area, Germany, and investigated N net mineralization, solute concentrations and fluxes of dissolved organic carbon (DOC) and of mineral ions (NH4 + , NO3 − , Na+ , K+ , Ca2+ , Mg2+ ). At the snow removal plots the minimum soil temperature was −5 °C at 5 cm depth, while the control plots were covered by snow and experienced no soil frost. The soil frost lasted for about 3 months and penetrated the soil to about 15 cm depth. In the 3 months after thawing, the in situ N net mineralization in the forest floor and upper mineral soil was not affected by soil frost. In late summer, NO3 − concentrations increased in forest floor percolates and soil solutions at 20 cm soil depth in the snow removal plots relative to the control. The increase lasted for about 2–4 months at a time of low seepage water fluxes. Soil frost did not affect DOC concentrations and radiocarbon signatures of DOC. No specific frost effect was observed for K+ , Ca2+ and Mg2+ in soil solutions, however, the Na+ concentrations in the upper mineral soil increased. In the 12 months following snowmelt, the solute fluxes of N, DOC, and mineral ions were not influenced by the previous soil frost at any depth. Our experiment did not support the hypothesis that moderate soil frost triggers solute losses of N, DOC, and mineral ions from temperate forest soils. 相似文献
33.
MERAV SEIFAN ARIEL GILAD KEREN KLASS YEHUDAH L. WERNER 《Biological journal of the Linnean Society. Linnean Society of London》2009,97(2):275-288
Recent arguments in the literature prompted us to compare methods for assessing sexual dimorphism in body proportions of lacertid lizards, using Acanthodactylus boskianus . Although expressing body-part measurements as proportional to head length was the most effective method, we recommend using trunk length for the baseline as a general method for lizards. We also argue that, when aiming to assess sexual dimorphism in body proportions of lizards, if the context is ecological, all available adults should be included. However, for morphology and taxonomy, small sub-samples of the largest individuals that maximally express their genetic morphological potential should be used. In A. boskianus , the sexual dimorphism of mensural characters in adults was typical: males were larger, with relatively larger head and appendages. However, the ontogeny of this dimorphism was unusual in that the differences existed already in youth and thereafter persisted isometrically. The sexual dimorphism of meristic characters was male-biased in numbers of femoral pores and of caudal vertebrae, and female-biased in numbers of ventral plates along the trunk and of precaudal vertebrae. Size dimorphism may conceivably play a role in sex recognition because two potential visual cues (i.e. size dimorphism and dichromatism) appear to complement each other. © 2009 The Linnean Society of London, Biological Journal of the Linnean Society 2009, 97 , 275–288. 相似文献
34.
Richard GH Immink Isabella AN Tonaco Stefan de Folter Anna Shchennikova Aalt DJ van Dijk Jacqueline Busscher-Lange Jan W Borst Gerco C Angenent 《Genome biology》2009,10(2):R24-16
Background
Plant MADS box proteins play important roles in a plethora of developmental processes. In order to regulate specific sets of target genes, MADS box proteins dimerize and are thought to assemble into multimeric complexes. In this study a large-scale yeast three-hybrid screen is utilized to provide insight into the higher-order complex formation capacity of the Arabidopsis MADS box family. SEPALLATA3 (SEP3) has been shown to mediate complex formation and, therefore, special attention is paid to this factor in this study. 相似文献35.
JF Yuan SJ Zhang O Jafer RA Furlong OE Chausiaux CA Sargent GH Zhang NA Affara 《BMC microbiology》2009,9(1):246
Background
Pseudorabies virus (PRV) is an alphaherpesviruses whose native host is pig. PRV infection mainly causes signs of central nervous system disorder in young pigs, and respiratory system diseases in the adult. 相似文献36.
Jamie Lovaglio James E Artwohl Christopher J Ward Thomas GH Diekwisch Yoshihiro Ito Jeffrey D Fortman 《Comparative medicine》2014,64(2):115-120
Three mice (2 male, 1 female; age, 5 to 16 mo) from a mouse line transgenic for keratin 14 (K14)-driven LacZ expression and on an outbred Crl:CD1(ICR) background, were identified as having distended abdomens and livers that were diffusely enlarged by numerous cysts (diameter, 0.1 to 2.0 cm). Histopathology revealed hepatic cysts lined by biliary type epithelium and mild chronic inflammation, and confirmed the absence of parasites. Among 21 related mice, 5 additional affected mice were identified via laparotomy. Breeding of these 5 mice (after 5 mo of age) did not result in any offspring; the K14 mice with polycystic livers failed to reproduce. Affected male mice had degenerative testicular lesions, and their sperm was immotile. Nonpolycystic K14 control male mice bred well, had no testicular lesions, and had appropriate sperm motility. Genetic analysis did not identify an association of this phenotype with the transgene or insertion site.Abbreviations: K14, keratin 14 promoter; LacZ, bacterial β-galactosidase LacZ reporter; Lsamp, mouse limbic system-associated membrane proteinPolycystic disease is a multiorgan disorder and is the most common genetic life-threatening disease in people, affecting more than 600,000 Americans.16 Cystic liver disease in people typically is associated with polycystic kidney disease22,36 but can exist in its absence. Currently, 2 autosomal dominant genes (PRKCSH and SEC63P) that cause a human polycystic liver disease condition without renal involvement have been identified.4-6,13Numerous rodent models of polycystic kidneys with concurrent polycystic liver exist.8,34,39,40 However, effective models of polycystic liver without polycystic kidneys would be useful to address clinical and mechanistic issues of polycystic liver not associated with polycystic kidneys.6,31Here we report multiple cases of a spontaneous polycystic liver phenotype without a kidney phenotype in a transgenic mouse line. We also describe the effect of the transgene on disease expression and our attempts to develop this stock as an animal model. 相似文献
37.
Sponge proteins are more similar to those of Homo sapiens than to Caenorhabditis elegans 总被引:2,自引:0,他引:2
VERA GAMULIN ISABEL M. MULLER WERNER E.G. MULLER 《Biological journal of the Linnean Society. Linnean Society of London》2000,71(4):821-828
We compared 42 phylogenetically conserved proteins from four marine sponges [Porifera] with almost the complete set of Caenorhabditis elegans proteins and all known proteins from humans. The majority of the sponge proteins arc significantly more similar to human than to C. elegans orthologucs/homologues. This finding reflects the accelerated evolutionary rate in the C. elegans lineage, since sponges split off first from the common ancestor of all multicellular animals. Furthermore, three sponge/human proteins were not found in C. elegans: (2–5)A synthetase, DNA repair helicase and lens βγ-crystallin. Sponges arc the source of the most ancient proteins already present in the common ancestor of all multicellular organisms. Some of these proteins were lost later during the evolution of individual animal lineages. These 'found/lost' proteins may serve as molecular markers for an improved systematics of Metazoa. In addition, phylogenetically conserved sponge proteins can be very helpful for the evaluation of differences in evolutionary rates in different animal lineages. We therefore propose sponges as the reference animals in molecular evolutionary studies of Metazoa. 相似文献
38.
Based on field measurements in two agriculturalecosystems, soil respiration and long-term response ofsoil organic carbon content (SOC) was modelled. Themodel predicts the influence of temperature increaseas well as the effects of land-use over a period ofthirty years in a northern German glacial morainelandscape. One of the fields carried a maizemonoculture treated with cattle slurry in addition tomineral fertilizer (maize monoculture), the otherwas managed by crop rotation and recieved organicmanure (crop rotation). The soils of both fieldswere classified as cambic Arenosols. The soilrespiration was measured in the fields by means of theopen dynamic inverted-box method and an infrared gasanalyser. The mean annual soil respiration rates were 268 (maizemonoculture) and 287 mg CO2 m-2 h-1(crop rotation). Factors controlling soil respirationwere soil temperature, soil moisture, root respirationand carbon input into the soil. Q10-valuesof soil respiration were generally higher in winterthan in summer. This trend is interpreted as anadaptive response of the soil microbial communities.In the model a novel mathematical approach withvariable Q10-values as a result oftemperature and moisture adjustment is proposed. Withthe calibrated model soil respiration and SOC werecalculated for both fields and simulations over aperiod of thirty years were established. Simulationswere based on (1) local climatic data, 1961 until1990, and (2) a regional climate scenario for northernGermany with an average temperature increase of 2.1 K.Over the thirty years period with present climateconditions, the SOC pool under crop rotation wasnearly stable due to the higher carbon inputs, whereasabout 16 t C ha-1 were lost under maizemonoculture. Under global warming the mean annualsoil respiration for both fields increased and SOCdecreased by ca. 10 t C ha-1 under croprotation and by more than 20 t C ha-1 undermaize monoculture. It was shown that overestimationof carbon losses in long-term prognoses can be avoidedby including a Q10-adjustment in soilrespiration models. 相似文献
39.
Thomas GH; Newbern EC; Korte CC; Bales MA; Muse SV; Clark AG; Kiehart DP 《Molecular biology and evolution》1997,14(12):1285-1295
Many structural, signaling, and adhesion molecules contain tandemly
repeated amino acid motifs. The alpha-actinin/spectrin/dystrophin
superfamily of F-actin-crosslinking proteins contains an array of triple
alpha-helical motifs (spectrin repeats). We present here the complete
sequence of the novel beta-spectrin isoform beta(Heavy)- spectrin (beta H).
The sequence of beta H supports the origin of alpha- and beta-spectrins
from a common ancestor, and we present a novel model for the origin of the
spectrins from a homodimeric actin-crosslinking precursor. The pattern of
similarity between the spectrin repeat units indicates that they have
evolved by a series of nested, nonuniform duplications. Furthermore, the
spectrins and dystrophins clearly have common ancestry, yet the repeat unit
is of a different length in each family. Together, these observations
suggest a dynamic period of increase in repeat number accompanied by
homogenization within each array by concerted evolution. However, today,
there is greater similarity of homologous repeats between species than
there is across repeats within species, suggesting that concerted evolution
ceased some time before the arthropod/vertebrate split. We propose a
two-phase model for the evolution of the spectrin repeat arrays in which an
initial phase of concerted evolution is subsequently retarded as each new
protein becomes constrained to a specific length and the repeats diverge at
the DNA level. This evolutionary model has general applicability to the
origins of the many other proteins that have tandemly repeated motifs.
相似文献
40.
A new group of darkly pigmented root-infecting fungi was isolated from cereal roots obtained from six different locations in northeastern Germany. Similar random amplified polymorphic DNA(RAPD) patterns and restriction profiles of amplified rDNA were used as a basis for classifying the isolates in a separate group. The isolates demonstrating mycelial and infection characteristics typical of Gaeumannomyces graminis could be differentiated from the varieties of G. graminis as well as from Gaeumannomyces cylindrosporus / Phialophora graminicola using RAPD Polymerase chain reaction (PCR) and rDNA Restriction-fragment length polymorphism (RFLP) analysis. Phylogenetic analysis of the Internal transcribed spacer (ITS) regions suggests that the isolates form a distinct group (named group 'E') situated within the Gaeumannomyces – Phialophora complex between the branch of the G. graminis varieties and Gaeumannomyces incrustans / Magnaporthe poae . Isolates of group E produced lobed hyphopodia and were shown in biotests to be non-pathogenic to wheat, oats, Italian Ryegrass and Chewings Fescue, suggesting it is a benign parasite which colonizes cereals or grasses without destroying vascular tissue. Furthermore, curved phialospores could be found. Summarizing the results presented, this new group could be classified as a new species of Phialophora . Although isolates of group E were found at only six of the 32 investigated locations, they composed up to 50% of total isolates of the Gaeumannomyces – Phialophora complex at these sites. Because of the non-pathogenic behaviour, the new group may be of value as biological control agents for pathogenic fungi. 相似文献