5-羟色胺诱发的主动脉区化学感受性升血压反射中某些区域性血管反应的分析

在15只麻醉开胸犬中,用在体器官恒流灌注法研究了骨胳肌、皮肤、肾和心脏的血管在5-HT 诱发的主动脉区化学感受性升血压反射中的反应,同时还观察了5-HT 对这些器官血管的直接效应。左心房注入5-HT(200μg)后,在动脉血压急剧上升的同时,股薄肌、皮肤(后爪)和肾脏的灌流压明显增加,其增值分别为43±8.47±5和56±27mmHg(P<0.01),而冠脉灌流压无明显改变。用阿托品和/或心得安阻断骨胳肌、皮肤和心脏灌流区域的 m 和同β-受体后,上述效应无进一步变动。向灌流环路直接注入5-HT(10—50μg),可引起皮肤和肾脏的血管收缩,而股薄肌和心肌的血管则扩张;后一效应不被心得安阻断。以上结果提示:1.在5-HT 诱发的主动脉区化学感受性升血压反射中,骨胳肌、皮肤和肾脏呈现明显的交感性缩血管反应,而并不伴有交感性扩血管活动;2.在外周血管可能存在两种不同的5-HT 受体,一种主要分布在皮肤和肾脏,引起缩血管效应;另一种主要分布于骨胳肌和冠状血管,引起扩血管效应。     

正常大鼠肾脏细胞溶酶体膜的构成蛋白

溶酶体是细胞内对其吞噬之物质溶解及消化之主要场所,同时也是细胞自噬作用的主要细胞器。为了进一步了解此细胞器的功能与结构,我们采用免疫荧光标记法,通过5种针对大鼠肝细胞溶酶体膜蛋白的特异性单克隆抗体,对大鼠正常肾脏细胞溶酶体膜蛋白进行了标记,并通过NH_4Cl溶液对溶酶体作了膜膨胀处理,结果显示:(1)细胞内溶酶体膜蛋白是由多种蛋白所构成,其各种蛋白的含量是不同的;(2)所有溶酶体膜蛋白均表达于该细胞器之表面;(3)NH_4Cl溶液能有效地使溶酶体扩张,这将有和于进一步研究溶酶体的结构。     

拟除虫菊酯类农药对茶树害虫的生物活性与残留降解

本文报道了溴氰菊酯、联苯菊酯、氯氰菊酯、顺式氯氰菊酯、杀灭菊酯、功夫菊酯和二氯苯醚菊酯等七种拟除虫菊酯类农药对茶树主要害虫的杀虫效果和在茶树叶片上的降解动态试验结果.上述七种拟除虫菊酯类农药对鳞翅目茶树害虫的幼虫都具有极高的毒力,但对其他害虫的活性谱则表现有差异.它们在茶树芽梢上的降解速率常数为0.20—0.28天^-1,在茶树上的半衰期为2.5—3.5天,明显较有机磷农药稳定.在影响降解的因素中,挥发、热分解、雨水淋洗的作用不大,由茶梢生长稀释所起的作用较为明显.鲜叶中的农药残留在加工过程中的降解率为15—45%,平均20%,远低于有机磷农药.成茶中的残留农药在泡茶过程中由于他们的低水溶性,因此浸出率很低,一般仅有1—4.5%.     

冬小麦春化过程中低温诱导的与花芽分化相关的mRNA和蛋白质的合成

应用SDS电泳及高分辨双向电泳技术,分析了冬小麦经春化、脱春化及超期春化处理后茎类蛋白质组分的变化。发现52.5,38kD和16.2kD蛋白质与冬小麦开花诱导密切相关。mRNA体外翻译结果表明,春化作用中低温诱导与开花紧密相关的mRNA的出现有一定的顺序性。因此推测:春化诱导开花是低温导致某些特定基因表达的结果,而表达的调节主要发生在转录水平上。     

樟叶蜂的生物学和防治

樟叶蜂是樟树幼苗的主要害虫之一。田间世代重叠。在广州,室内饲养年1—7代,以老熟幼虫于土中茧内越冬。卵单个地散产于嫩叶组织内,幼虫取食嫩叶,对樟树幼苗造成严重损害。幼虫老熟时下地入土结茧。两性或孤雌生殖。幼虫四龄。预蛹期常滞育。 室内试验表明:对2—3龄幼虫喷射90%敌百虫晶体100,000倍或200,000倍液;80%敌敌畏乳剂100,000倍液:40%乐果乳剂30,000倍液;50%杀螟松乳剂10,000或20,000倍液:50%马拉松乳剂10,000或20,000倍液;80%易卫杀可溶性粉5,00或10,000倍液;25%可湿性西维因2,000或4,000倍液;20%除虫菊脂乳剂2,000或4,000倍液;以及27.5%杀虫双乳剂10,000或20,000倍液或25%杀虫净乳剂2,000或4,000倍液(加洗衣粉0.025%)均有良好效果。     

Comprehensive profiling of accessible surface glycans of mammalian sperm using a lectin microarray

It is well known that cell surface glycans or glycocalyx play important roles in sperm motility, maturation and fertilization. A comprehensive profile of the sperm surface glycans will greatly facilitate both basic research (sperm glycobiology) and clinical studies, such as diagnostics of infertility. As a group of natural glycan binders, lectin is an ideal tool for cell surface glycan profiling. However, because of the lack of effective technology, only a few lectins have been tested for lectin-sperm binding profiles. To address this challenge, we have developed a procedure for high-throughput probing of mammalian sperm with 91 lectins on lectin microarrays. Normal sperm from human, boar, bull, goat and rabbit were collected and analyzed on the lectin microarrays. Positive bindings of a set of ~50 lectins were observed for all the sperm of 5 species, which indicated a wide range of glycans are on the surface of mammalian sperm. Species specific lectin bindings were also observed. Clustering analysis revealed that the distances of the five species according to the lectin binding profiles are consistent with that of the genome sequence based phylogenetic tree except for rabbit. The procedure that we established in this study could be generally applicable for sperm from other species or defect sperm from the same species. We believe the lectin binding profiles of the mammalian sperm that we established in this study are valuable for both basic research and clinical studies.     

Bmp2 regulates Serpinb6b expression via cAMP/PKA/Wnt4 pathway during uterine decidualization

Serpinb6b is a novel member of Serpinb family and found in germ and somatic cells of mouse gonads, but its physiological function in uterine decidualization remains unclear. The present study revealed that abundant Serpinb6b was noted in decidual cells, and advanced the proliferation and differentiation of stromal cells, indicating a creative role of Serpinb6b in uterine decidualization. Further analysis found that Serpinb6b modulated the expression of Mmp2 and Mmp9. Meanwhile, Serpinb6b was identified as a target of Bmp2 regulation in stromal differentiation. Treatment with rBmp2 resulted in an accumulation of intracellular cAMP level whose function in this differentiation program was mediated by Serpinb6b. Addition of PKA inhibitor H89 impeded the Bmp2 induction of Serpinb6b, whereas 8‐Br‐cAMP rescued the defect of Serpinb6b expression elicited by Bmp2 knock‐down. Attenuation of Serpinb6b greatly reduced the induction of constitutive Wnt4 activation on stromal cell differentiation. By contrast, overexpression of Serpinb6b prevented this inhibition of differentiation process by Wnt4 siRNA. Moreover, blockage of Wnt4 abrogated the up‐regulation of cAMP on Serpinb6b. Collectively, Serpinb6b mediates uterine decidualization via Mmp2/9 in response to Bmp2/cAMP/PKA/Wnt4 pathway.     

Exploring Lassa Virus Proteome to Design a Multi-epitope Vaccine Through Immunoinformatics and Immune Simulation Analyses

International Journal of Peptide Research and Therapeutics - Lassa virus (LASV) is responsible for a type of acute viral haemorrhagic fever referred to as Lassa fever. Lack of adequate treatment...